Original Article

Planta

, Volume 236, Issue 6, pp 1775-1790

Physiological responses and endogenous cytokinin profiles of tissue-cultured ‘Williams’ bananas in relation to roscovitine and an inhibitor of cytokinin oxidase/dehydrogenase (INCYDE) treatments

  • Adeyemi O. AremuAffiliated withResearch Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal
  • , Michael W. BairuAffiliated withResearch Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal
  • , Ondřej NovákAffiliated withLaboratory of Growth Regulators, Institute of Experimental Botany AS CR, Palacký University
  • , Lenka PlačkováAffiliated withLaboratory of Growth Regulators, Institute of Experimental Botany AS CR, Palacký UniversityFaculty of Science, Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University
  • , Marek ZatloukalAffiliated withLaboratory of Growth Regulators, Institute of Experimental Botany AS CR, Palacký UniversityFaculty of Science, Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University
  • , Karel DoležalAffiliated withLaboratory of Growth Regulators, Institute of Experimental Botany AS CR, Palacký UniversityFaculty of Science, Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University
  • , Jeffrey F. FinnieAffiliated withResearch Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal
  • , Miroslav StrnadAffiliated withLaboratory of Growth Regulators, Institute of Experimental Botany AS CR, Palacký UniversityFaculty of Science, Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University
  • , Johannes Van StadenAffiliated withResearch Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal Email author 

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Abstract

The effect of supplementing either meta-topolin (mT) or N 6-benzyladenine (BA) requiring cultures with roscovitine (6-benzylamino-2-[1(R)-(hydroxymethyl)propyl]amino-9-isopropylpurine), a cyclin-dependent kinase (CDK) and N-glucosylation inhibitor, and INCYDE (2-chloro-6-(3-methoxyphenyl)aminopurine), an inhibitor of cytokinin (CK) degradation, on the endogenous CK profiles and physiology of banana in vitro was investigated. Growth parameters including multiplication rate and biomass were recorded after 42 days. Endogenous CK levels were quantified using UPLC–MS/MS while the photosynthetic pigment and phenolic contents were evaluated spectrophotometrically. The highest regeneration rate (93 %) was observed in BA + roscovitine while mT + INCYDE plantlets produced most shoots. Treatment with BA + roscovitine had the highest shoot length and biomass. Although not significant, there was a higher proanthocyanidin level in BA + roscovitine treatments compared to the control (BA). The levels of total phenolics and flavonoids were significantly higher in mT + roscovitine treatment than in the mT-treated regenerants. The presence of roscovitine and/or INCYDE had no significant effect on the photosynthetic pigments of the banana plantlets. Forty-seven aromatic and isoprenoid CKs categorized into nine CK-types were detected at varying concentrations. The presence of mT + roscovitine and/or INCYDE increased the levels of O-glucosides while 9-glucosides were higher in the presence of BA. Generally, the underground parts had higher CK levels than the aerial parts; however, the presence of INCYDE increased the level of CK quantified in the aerial parts. From a practical perspective, the use of roscovitine and INCYDE in micropropagation could be crucial in the alleviation of commonly observed in vitro-induced physiological abnormalities.

Keywords

Cyclin-dependent kinase Cytokinin metabolism Micropropagation Musa spp. Photosynthesis Plant secondary metabolites