Planta

, Volume 236, Issue 4, pp 1037–1051

Expression of flavonoid genes in the red grape berry of ‘Alicante Bouschet’ varies with the histological distribution of anthocyanins and their chemical composition

  • Luigi Falginella
  • Gabriele Di Gaspero
  • Simone Diego Castellarin
Original Article

DOI: 10.1007/s00425-012-1658-2

Cite this article as:
Falginella, L., Di Gaspero, G. & Castellarin, S.D. Planta (2012) 236: 1037. doi:10.1007/s00425-012-1658-2

Abstract

The mature berry of Vitis vinifera ‘Alicante Bouschet’ is entirely red, but anthocyanin metabolism discloses elements of histological discontinuity. This provides an experimental system amenable to studies of compartmentalised secondary metabolism in a fleshly fruit. We compared microscopy of fixed berry sections and chemical composition of anthocyanin extracts with the expression of 41 flavonoid genes in three berry tissues. In the pericarp, anthocyanins formed membrane-encased spherical coalescences that gradually enlarged and were shuttled into the vacuolar system. The size and the intensity of in situ pigmentation and of colour extracts of anthocyanin vesicles all decreased with depth beneath the epidermis. Shades of red colour, and the quantity and types of anthocyanins in skin, flesh, and seed extracts were correlated with differences in the expression of flavonoid 3′,5′-hydroxylases and anthocyanin genes encoding transcription factors, enzymatic proteins, and transporters. Fine adjustments in the global transcriptional modulation of the pathway occurred distinctively in each tissue, within four groups of co-expressed genes that were more associated with either the pericarp or the seed, and with either early or late-ripening stages. All structural genes controlling early steps of the flavonoid pathway exist in the grapevine genome in multiple copies that were recruited by antagonistic branches of the pathway in the ‘Alicante Bouschet’ berry. Expression patterns of individual paralogs were spatiotemporally distinct from one another, in step with either anthocyanin genes or proanthocyanidin genes.

Keywords

Fruit Grapevine Ripening Secondary metabolism Vitis vinifera 

Supplementary material

425_2012_1658_MOESM1_ESM.pdf (737 kb)
Supplementary material 1 (PDF 737 kb)
425_2012_1658_MOESM2_ESM.pdf (1.1 mb)
Supplementary material 2 (PDF 1168 kb)
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Supplementary material 3 (PDF 104 kb)
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Supplementary material 4 (PDF 883 kb)
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Supplementary material 5 (PDF 521 kb)
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Supplementary material 6 (PDF 1004 kb)
425_2012_1658_MOESM7_ESM.pdf (179 kb)
Supplementary material 7 (PDF 179 kb)

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Luigi Falginella
    • 1
  • Gabriele Di Gaspero
    • 1
    • 2
  • Simone Diego Castellarin
    • 1
  1. 1.Dipartimento di Scienze Agrarie e AmbientaliUniversity of UdineUdineItaly
  2. 2.Istituto di Genomica ApplicataParco Scientifico e Tecnologico Luigi DanieliUdineItaly

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