Planta

, Volume 235, Issue 6, pp 1431–1447

Transcriptional response of Medicago truncatula sulphate transporters to arbuscular mycorrhizal symbiosis with and without sulphur stress

Rapid Communication

DOI: 10.1007/s00425-012-1645-7

Cite this article as:
Casieri, L., Gallardo, K. & Wipf, D. Planta (2012) 235: 1431. doi:10.1007/s00425-012-1645-7

Abstract

Sulphur is an essential macronutrient for plant growth, development and response to various abiotic and biotic stresses due to its key role in the biosynthesis of many S-containing compounds. Sulphate represents a very small portion of soil S pull and it is the only form that plant roots can uptake and mobilize through H+-dependent co-transport processes implying sulphate transporters. Unlike the other organically bound forms of S, sulphate is normally leached from soils due to its solubility in water, thus reducing its availability to plants. Although our knowledge of plant sulphate transporters has been growing significantly in the past decades, little is still known about the effect of the arbuscular mycorrhiza interaction on sulphur uptake. Carbon, nitrogen and sulphur measurements in plant parts and expression analysis of genes encoding putative Medicago sulphate transporters (MtSULTRs) were performed to better understand the beneficial effects of mycorrhizal interaction on Medicago truncatula plants colonized by Glomus intraradices at different sulphate concentrations. Mycorrhization significantly promoted plant growth and sulphur content, suggesting increased sulphate absorption. In silico analyses allowed identifying eight putative MtSULTRs phylogenetically distributed over the four sulphate transporter groups. Some putative MtSULTRs were transcribed differentially in roots and leaves and affected by sulphate concentration, while others were more constitutively transcribed. Mycorrhizal-inducible and -repressed MtSULTRs transcripts were identified allowing to shed light on the role of mycorrhizal interaction in sulphate uptake.

Keywords

Arbuscular mycorrhizaGlomus intraradicesMedicago truncatulaSulphateTransporters

Abbreviations

AM

Arbuscular mycorrhiza

AtSULTR

Arabidopsis thaliana sulphate transporter

CNS

Carbon, nitrogen, sulphur

CT

Cycle threshold

DW

Dry weight

FW

Fresh weight

Mt

Medicago truncatula

MtBcp1a

Medicago truncatula blue copper protein 1a

MtPT4

Medicago truncatula phosphate transporter 4

MtSULTR

Medicago truncatula sulphate transporter

Myc

Mycorrhized

Myc:Myc

Mycorrhized compartments of Myc plants

NM

Non-mycorrhized

NM:Myc

Non-mycorrhized compartments of Myc plants

NM:NM

Non-mycorrhized compartments of NM plants

OsSULTR

Orzya sativa sulphate transporter

qPCR

Quantitative real time polymerase chain reaction

SULTR

Sulphate transporter

Wpi

Weeks post inoculation

Supplementary material

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Supplementary material 1 (TXT 39 kb)
425_2012_1645_MOESM2_ESM.doc (134 kb)
Supplementary material 2 (DOC 134 kb)
425_2012_1645_MOESM3_ESM.doc (160 kb)
Supplementary material 3 (DOC 159 kb)
425_2012_1645_MOESM4_ESM.doc (126 kb)
Supplementary material 4 (DOC 126 kb)
425_2012_1645_MOESM5_ESM.doc (172 kb)
Supplementary material 5 (DOC 172 kb)
425_2012_1645_MOESM6_ESM.doc (188 kb)
Supplementary material 6 (DOC 188 kb)
425_2012_1645_MOESM7_ESM.doc (204 kb)
Supplementary material 7 (DOC 204 kb)

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Leonardo Casieri
    • 1
  • Karine Gallardo
    • 2
  • Daniel Wipf
    • 1
  1. 1.Pôle Interactions Plantes-Microorganismes, ERL 6300 CNRSUMR1347 INRA/Agrosup/Université de Bourgogne AgroécologieDijon CedexFrance
  2. 2.Pôle GEAPSIUMR1347 INRA/Agrosup/Université de Bourgogne AgroécologieDijon CedexFrance