Planta

, 234:903

Peroxisomal localisation of the final steps of the mevalonic acid pathway in planta

  • Andrew J. Simkin
  • Grégory Guirimand
  • Nicolas Papon
  • Vincent Courdavault
  • Insaf Thabet
  • Olivia Ginis
  • Sadok Bouzid
  • Nathalie Giglioli-Guivarc’h
  • Marc Clastre
Original Article

DOI: 10.1007/s00425-011-1444-6

Cite this article as:
Simkin, A.J., Guirimand, G., Papon, N. et al. Planta (2011) 234: 903. doi:10.1007/s00425-011-1444-6

Abstract

In plants, the mevalonic acid (MVA) pathway provides precursors for the formation of triterpenes, sesquiterpenes, phytosterols and primary metabolites important for cell integrity. Here, we have cloned the cDNA encoding enzymes catalysing the final three steps of the MVA pathway from Madagascar periwinkle (Catharanthus roseus), mevalonate kinase (MVK), 5-phosphomevalonate kinase (PMK) and mevalonate 5-diphosphate decarboxylase (MVD). These cDNA were shown to functionally complement MVA pathway deletion mutants in the yeast Saccharomyces cerevisiae. Transient transformations of C. roseus cells with yellow fluorescent protein (YFP)-fused constructs reveal that PMK and MVD are localised to the peroxisomes, while MVK was cytosolic. These compartmentalisation results were confirmed using the Arabidopsis thaliana MVK, PMK and MVD sequences fused to YFP. Based on these observations and the arguments raised here we conclude that the final steps of the plant MVA pathway are localised to the peroxisome.

Keywords

ArabidopsisCatharanthusIsoprenoidMevalonic acid pathwayPeroxisome

Abbreviations

AACT

Acetoacetyl-CoA thiolase

FOA

5-fluoroorotic acid

HMGR

3-hydroxy-3-methylglutaryl-CoA reductase

HMGS

3-hydroxy-3-methylglutaryl-CoA synthase

IDI

Isopentenyl diphosphate isomerase

MVA

Mevalonic acid

MVD

Mevalonate 5-diphosphate decarboxylase

MVK

Mevalonate kinase

PMK

5-phosphomevalonate kinase

PTS

Peroxisomal targeting signal

YFP

Yellow fluorescent protein

Supplementary material

425_2011_1444_MOESM1_ESM.doc (234 kb)
Supplementary material 1 (DOC 234 kb)
425_2011_1444_MOESM2_ESM.doc (76 kb)
Supplementary material 2 (DOC 76 kb)
425_2011_1444_MOESM3_ESM.doc (72 kb)
Supplementary material 3 (DOC 71 kb)

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Andrew J. Simkin
    • 1
  • Grégory Guirimand
    • 1
  • Nicolas Papon
    • 1
  • Vincent Courdavault
    • 1
  • Insaf Thabet
    • 1
    • 2
  • Olivia Ginis
    • 1
  • Sadok Bouzid
    • 2
  • Nathalie Giglioli-Guivarc’h
    • 1
  • Marc Clastre
    • 1
  1. 1.EA 2106, Biomolécules et Biotechnologies VégétalesUniversité François-Rabelais de ToursToursFrance
  2. 2.Laboratoire de Biotechnologie et Physiologie Végétale, Département des Sciences BiologiquesFaculté des Sciences de TunisTunisTunisia