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Environmental regulation of stomatal response in the Arabidopsis Cvi-0 ecotype

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Abstract

The Arabidopsis Cape Verde Islands (Cvi-0) ecotype is known to differ from other ecotypes with respect to environmental stress responses. We analyzed the stomatal behavior of Cvi-0 plants, in response to environmental signals. We investigated the responses of stomatal conductance and aperture to high [CO2] in the Cvi-0 and Col-0 ecotypes. Cvi-0 showed constitutively higher stomatal conductance and more stomatal opening than Col-0. Cvi-0 stomata opened in response to light, but the response was slow. Under low humidity, stomatal opening was increased in Cvi-0 compared to Col-0. We then assessed whether low humidity affects endogenous ABA levels in Cvi-0. In response to low humidity, Cvi-0 had much higher ABA levels than Col-0. However, epidermal peels experiments showed that Cvi-0 stomata were insensitive to ABA. Measurements of organic and inorganic ions in Cvi-0 guard cell protoplasts indicated an over-accumulation of osmoregulatory anions (malate and Cl−). This irregular anion homeostasis in the guard cells may explain the constitutive stomatal opening phenotypes of the Cvi-0 ecotype, which lacks high [CO2]-induced and low humidity-induced stomatal closure.

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Abbreviations

ABA:

Abscisic acid

FACE:

Free air CO2 enrichment

FW:

Fresh weight

GCP:

Guard cell protoplast

g s :

Stomatal conductance

JA:

Jasmonic acid

O3 :

Ozone

RH:

Relative humidity

SA:

Salicylic acid

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Acknowledgments

We thank Drs. Nobuharu Goto and Osamu Matsuda for their helpful discussion. We also thank Ms. Naomi Kawahara for the technical assistance. This work was supported by a Grant-in-Aid for Scientific Research on Innovative Areas (No. 21114002) from the Ministry of Education, Science and Culture of Japan, and by the Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry (BRAIN).

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Authors and Affiliations

  1. Department of Biology, Faculty of Sciences, Kyushu University, Fukuoka, 812-8581, Japan

    Keina Monda, Juntaro Negi, Kensuke Kusumi, Mimi Hashimoto & Koh Iba

  2. National Institute for Environmental Studies (NIES), Tsukuba, 305-8506, Japan

    Atsuhiro Iio

  3. RIKEN Plant Science Center, Yokohama, 230-0045, Japan

    Mikiko Kojima & Hitoshi Sakakibara

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  1. Keina Monda
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  2. Juntaro Negi
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  3. Atsuhiro Iio
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  4. Kensuke Kusumi
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  6. Mimi Hashimoto
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  7. Hitoshi Sakakibara
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  8. Koh Iba
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Correspondence to Koh Iba.

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Monda, K., Negi, J., Iio, A. et al. Environmental regulation of stomatal response in the Arabidopsis Cvi-0 ecotype. Planta 234, 555–563 (2011). https://doi.org/10.1007/s00425-011-1424-x

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