Planta

, Volume 232, Issue 6, pp 1423–1432

Salicylate-mediated suppression of jasmonate-responsive gene expression in Arabidopsis is targeted downstream of the jasmonate biosynthesis pathway

  • Antonio Leon-Reyes
  • Dieuwertje Van der Does
  • Elvira S. De Lange
  • Carolin Delker
  • Claus Wasternack
  • Saskia C. M. Van Wees
  • Tita Ritsema
  • Corné M. J. Pieterse
Open AccessOriginal Article

DOI: 10.1007/s00425-010-1265-z

Cite this article as:
Leon-Reyes, A., Van der Does, D., De Lange, E.S. et al. Planta (2010) 232: 1423. doi:10.1007/s00425-010-1265-z

Abstract

Jasmonates (JAs) and salicylic acid (SA) are plant hormones that play pivotal roles in the regulation of induced defenses against microbial pathogens and insect herbivores. Their signaling pathways cross-communicate providing the plant with a regulatory potential to finely tune its defense response to the attacker(s) encountered. In Arabidopsis thaliana, SA strongly antagonizes the jasmonic acid (JA) signaling pathway, resulting in the downregulation of a large set of JA-responsive genes, including the marker genes PDF1.2 and VSP2. Induction of JA-responsive marker gene expression by different JA derivatives was equally sensitive to SA-mediated suppression. Activation of genes encoding key enzymes in the JA biosynthesis pathway, such as LOX2, AOS, AOC2, and OPR3 was also repressed by SA, suggesting that the JA biosynthesis pathway may be a target for SA-mediated antagonism. To test this, we made use of the mutant aos/dde2, which is completely blocked in its ability to produce JAs because of a mutation in the ALLENE OXIDE SYNTHASE gene. Mutant aos/dde2 plants did not express the JA-responsive marker genes PDF1.2 or VSP2 in response to infection with the necrotrophic fungus Alternaria brassicicola or the herbivorous insect Pieris rapae. Bypassing JA biosynthesis by exogenous application of methyl jasmonate (MeJA) rescued this JA-responsive phenotype in aos/dde2. Application of SA suppressed MeJA-induced PDF1.2 expression to the same level in the aos/dde2 mutant as in wild-type Col-0 plants, indicating that SA-mediated suppression of JA-responsive gene expression is targeted at a position downstream of the JA biosynthesis pathway.

Keywords

Hormone crosstalkJasmonic acidPlant defenseSalicylic acid

Abbreviations

AOS

Allene oxide synthase

JA

Jasmonic acid

JAs

Jasmonates

MeJA

Methyl jasmonate

SA

Salicylic acid

Introduction

Plants are at constant risk of being attacked by a wide variety of insect herbivores and microbial pathogens. To defend themselves, plants possess a powerful innate immune system by which they recognize non-self molecules or signals from injured cells, and respond by activating an effective defense response (Jones and Dangl 2006; Howe and Jander 2008). The importance of the phytohormones salicylic acid (SA), jasmonic acid (JA), ethylene, and abscisic acid as primary signals in the regulation of the plant’s immune response is well established (Pieterse et al. 2009; Verhage et al. 2010). Upon pathogen or insect attack, the quantity, composition, and timing of the phytohormonal blend produced by the plant depends greatly on the lifestyle and infection strategy of the invading attacker. This so-called ‘signal signature’ results in the activation of a specific set of defense-related genes that eventually determines the nature and effectiveness of the immune response that is triggered by the invader (De Vos et al. 2005). In recent years, molecular, genetic, and genomic tools have been used to uncover the complexity of the hormone-regulated induced defense signaling network. Besides balancing the relative abundance of different hormones, intensive interplay between hormone signaling pathways emerged as an important regulatory mechanism by which the plant is able to tailor its immune response to the type of invader encountered (Pieterse et al. 2009). Pathogens and insects, on the other hand, can manipulate the plant’s defense signaling network for their own benefit by affecting phytohormone homeostasis to antagonize the host immune response (Pieterse and Dicke 2007; Walling 2008; Grant and Jones 2009).

JA, its structurally related metabolites, and the JA precursor 12-oxo-phytodienoic acid (OPDA) (here collectively called jasmonates, JAs) are lipid-derived compounds that upon pathogen or insect attack are rapidly synthesized via the oxylipin biosynthesis pathway (reviewed in Wasternack 2007; Gfeller et al. 2010). Mutants of Arabidopsis thaliana (Arabidopsis) defective in JA biosynthesis or signaling revealed important roles of JAs in defense against nectrotrophic pathogens and herbivorous insects (Browse 2009; Van der Ent et al. 2009b). ALLENE OXIDE SYNTHASE (AOS) emerged as a key enzyme in the JA biosynthesis pathway, since mutation of the single AOS gene in Arabidopsis leads to a complete elimination of JA production (Park et al. 2002; Von Malek et al. 2002). Upon synthesis, JA can be readily metabolized to the volatile methyl jasmonate (MeJA) through the activity of JA carboxyl methyltransferase (JMT) (Seo et al. 2001). In addition JA can be conjugated to amino acids such as isoleucine via the activity of the JA conjugate synthase JAR1 (Staswick and Tiryaki 2004), resulting in the biologically highly active form (+)-7-iso-jasmonoyl-L-isoleucine (JA-Ile) (Fonseca et al. 2009). The F-box protein COI1 (CORONATINE INSENSITIVE 1 ) is a key regulator of the JA signaling pathway (Chung et al. 2009). It functions as a JA-Ile receptor in the E3 ubiquitin-ligase Skip-Cullin-F-box complex SCFCOI1 (Yan et al. 2009). Binding of JA-Ile to COI1 leads to degradation of JASMONATE ZIM-domain (JAZ) transcriptional repressor proteins via the proteasome (Chini et al. 2007; Thines et al. 2007). In resting cells, JAZ proteins act as transcriptional repressors of JA signaling by binding to positive transcriptional regulators, such as MYC2 (Chini et al. 2007). Hence, JAZ protein degradation results in de-repression of the JA signaling pathway and the activation of a large number of JA-responsive genes (Memelink 2009).

The JA-related transcriptome of Arabidopsis has been well studied. Strikingly, transcriptional changes in response to diverse JA-inducing biotic agents show limited overlap, suggesting that the context in which the JA signal is perceived is crucial in shaping the JA response (De Vos et al. 2005; Pozo et al. 2008; Pauwels et al. 2009). During plant interactions with pathogens and insects, the balance of different JA derivatives can rapidly change. Different JAs differentially activate JA responsive genes, which may fine-tune the JA response (Farmer et al. 2003; Gfeller et al. 2010). Moreover, JAs are often produced in combination with other hormones of which SA emerged as an important antagonist of the JA response (Koornneef and Pieterse 2008). Early studies in tomato revealed that SA and its acetylated form aspirin, are potent suppressors of the JA-dependent wound response (Doherty et al. 1988; Peña-Cortés et al. 1993). Also in Arabidopsis, SA was shown to suppress JA signaling, resulting in the downregulation of JA-responsive gene expression (Van Wees et al. 1999; Gupta et al. 2000; Spoel et al. 2003). In Arabidopsis, the JA-responsive marker genes PLANT DEFENSIN 1.2 (PDF1.2) and VEGETATIVE STORAGE PROTEIN 2 (VSP2) are highly sensitive to suppression by exogenous application of SA (Spoel et al. 2003; Koornneef et al. 2008; Leon-Reyes et al. 2009, 2010; Zander et al. 2009). This antagonism between SA and JA signaling was observed in a large number of Arabidopsis accessions collected from very different geographic origins (Koornneef et al. 2008), highlighting the potential significance of SA–JA crosstalk in nature. Indeed, trade-offs between SA-dependent resistance to biotrophic pathogens and JA-dependent defense against insect herbivory or infection by necrotrophic pathogens have been repeatedly reported (Bostock 2005; Verhage et al. 2010). In Arabidopsis, the SA pathway has been shown to inhibit JA-dependent resistance against the tissue-chewing herbivores Spodoptera exigua (beet armyworm) (Cipollini et al. 2004; Bodenhausen and Reymond 2007; Van Oosten et al. 2008), Spodoptera littoralis (Egyptian cotton worm) (Bruessow et al. 2010), and Trichoplusia ni (cabbage looper) (Cui et al. 2002), the cell-content feeding insects Frankliniella occidentalis (Western flower thrips) (Leon-Reyes et al. 2009) and silverleaf whitefly (Bremia tabaci) (Zarate et al. 2007), and the necrotrophic fungus Alternaria brassicicola (Spoel et al. 2007; Leon-Reyes et al. 2009).

The antagonistic effect of SA on JA signaling in plants shows a remarkable resemblance to the effect of the anti-inflammatory drug aspirin (acetyl-SA) on the formation of prostaglandins in animal cells. Prostaglandins are hormonal pain messengers that are structurally related to JAs and play a role in inflammation at sites of infection or tissue injury (Straus and Glass 2001). JAs and prostaglandins are both synthesized via the oxylipin biosynthesis pathway in which the enzymatic reactions leading to JA and prostaglandin formation are similar (Pan et al. 1998). In animal cells, aspirin antagonizes prostaglandin action by targeting enzyme activity and gene expression of CYCLOOXYGENASE (Straus and Glass 2001), the counterpart of AOS in plants. Although no inhibitory effect of SA on AOS enzyme activity has been observed in plants (Laudert and Weiler 1998), SA has been shown to suppress JA biosynthesis (Peña-Cortés et al. 1993; Spoel et al. 2003; Norton et al. 2007). Hence, antagonism of JA biosynthesis may be an important factor in the suppression of JA signaling by the SA pathway.

In Arabidopsis, induction of the JA response results in the activation of several JA biosynthesis genes, such as LOX2 (LIPOXYGENASE2), AOS, AOC2 (ALLENE OXIDE CYCLASE2), and OPR3 (12-OXO-PHYTODIENOATE REDUCTASE3) (Sasaki et al. 2001; Stenzel et al. 2003; Wasternack 2007). This indicates that JA biosynthesis is under the control of a positive feedback regulatory system, and that downregulation of JA biosynthesis gene expression by SA may be an important mechanism in SA–JA crosstalk. We reasoned that if the JA biosynthesis pathway is an important target of SA in the suppression of the JA signaling pathway, then Arabidopsis genotypes affected in JA biosynthesis should be significantly impaired in SA-mediated suppression of the JA response. In this study we tested this hypothesis and provided evidence that the antagonistic effect of SA on JA-dependent defense-related gene expression does not require downregulation of the JA biosynthesis pathway.

Materials and methods

Plant material

Seeds of A. thaliana genotypes were sown in quartz sand. Two-week-old seedlings were transferred to 60-mL pots containing a sand/potting soil mixture that was autoclaved twice for 20 min. Plants were cultivated in a growth chamber with an 8-h day (24°C) and 16-h night (20°C) cycle at 70% relative humidity for another 3 weeks. Plants were watered every other day and received half-strength Hoagland nutrient solution containing 10 mM Sequestreen (Ciba-Geigy, Frankfurt, Germany) once a week. For all the experiments 5-week-old soil-grown plants were used. The following Arabidopsis genotypes were used: wild-type accessions Col-0, Col-5, and Ws-0 (Nottingham Arabidopsis Stock Centre, UK), mutants aos/dde2-2 [Col-0] (Von Malek et al. 2002), opr3 [Ws-0] (Stintzi and Browse 2000), aim1 [Ws-0] (Richmond and Bleecker 1999), jar1-1 [Col-0] (Staswick et al. 1992) and npr1-1 [Col-0] (Cao et al. 1994), AOC::RNAi lines 5 and 16 [Col-0] (Delker 2005), and the LOX2 co-suppressed anti-sense transgenic line S-12 [Col-5] (Bell and Mullet 1993). The following T-DNA knockout lines [Col-0] were obtained from the SALK Institute Genomic Analysis Institute: SALK_140659 for opcl1 (At1g20510) (Koo et al. 2006) and At1g19640 Exotic line SM_3_35279 for jmt. Disruption of the JMT gene was checked by PCR using a specific primer for the insert (Spm32exotic FOR 5′- TAC GAA TAA GAG CGT CCA TTT TAG AGT GA -3′) and a JMT-specific primer (JMT REV1 5′- TGT TTT TGG TAA TTT AAA CTA GTT TCT TG -3′). Gene-specific primers for JMT (JMT FOR2; 5′- GCA CCA ACT CCT AAG TGG CAA G -3′; JMT REV2; 5′-AAA GAA GCA AGG TAT GGC AGT AAA ACA TT-3′) were used as controls for the endogenous gene. For seed production, sterility of the mutants aos/dde2 and opr3 was restored by exogenous application of MeJA to the flowers as described (Stintzi and Browse 2000; Park et al. 2002; Von Malek et al. 2002).

Pathogen and insect assays

Alternaria brassicicola strain MUCL 20297 was grown on potato dextrose agar (PDA; Difco Laboratories, Detroit, MI, USA) plates for 2 weeks at 22°C and conidia were subsequently collected as described (Broekaert et al. 1990). Plants were inoculated when 5 weeks old by applying 5 μL droplets of half-strength potato dextrose broth containing 5 × 105 spores per mL, as described previously (Van der Ent et al. 2008). After inoculation, plants were kept at 100% relative humidity for optimal infection. Hyaloperonospora arabidopsidis strain WACO9 was cultivated and used for plant inoculations as described (Van der Ent et al. 2009a). In the multi-attacker experiment, H. arabidopsidis was inoculated 72 h prior to Alternaria brassicicola.Pieris rapae were reared as described previously (De Vos et al. 2005) and transferred to 5-week-old plants. Infestation was carried out by transferring five first-instar larvae of P. rapae to each plant using a fine paintbrush.

Chemical treatments

Plants were treated with SA (Mallinckrodt Baker, Deventer, The Netherlands) and/or MeJA (Duchefa Biochemie BV, Haarlem, The Netherlands) by dipping the leaves into a solution of 0.015% (v/v) Silwet L77 (Van Meeuwen Chemicals BV, Weesp, The Netherlands) containing 0.1 mM MeJA, 1 mM SA or a combination of both chemicals as described previously (Spoel et al. 2003; Leon-Reyes et al. 2009). Control treatments were dipped into a solution containing 0.015% (v/v) Silwet L77. MeJA was added to the solutions from a 1,000-fold concentrated stock in 96% ethanol. To the solutions without MeJA, a similar volume of 96% ethanol was added. Treatments with 0.1 mM linolenic acid (LA) (Sigma-Aldrich, St. Louis, USA), OPDA, JA, and JA-Ile were performed as described for MeJA. The compounds were synthesized as described (Miersch et al. 2008; Fonseca et al. 2009).

RNA extraction and Northern-blot analysis

For RNA extraction, at least five plants per treatment were harvested at the time points indicated. RNA isolation and Northern-blot analysis were performed, as described previously (Van Wees et al. 1999). Northern blots were hybridized with gene-specific probes for PR-1 (At2g14610), PDF1.2 (At5g44420), VSP2 (At5g24770), LOX2 (At3g45140), and 18S rRNA as described (Van Wees et al. 1999; Pozo et al. 2008). Probes for the genes AOS (At5g42650), OPR3 (At2g06050), and AOC2 (At3g25770) were made by PCR amplification on cDNA that was synthesized from mRNA which was isolated from MeJA-treated Arabidopsis plants. Primers used for the amplification of gene-specific probes were: AOS-FOR 5′-CCC TTT TCC GAT TTC TCT CC-3′ and AOS-REV 5′-ACG GTA GCC TCC GGT TAG TT-3′, OPR3-FOR 5′-AAA ACA GGT GGC GAG TTT TG-3′ and OPR3-REV 5′-GCC TTC CAG ACT CTG TTT GC-3′, AOC2-FOR 5′-GCC AAG AAG AAC CTC ACT GC-3′ and AOC2-REV 5′-GGC ACC TTC AAA GAT TCC AG-3′. To check for equal loading, blots were stripped and hybridized with a probe for 18S ribosomal RNA. After hybridization with α-32P-dCTP-labeled probes, blots were exposed for autoradiography. Signal intensities of probes were quantified using a BioRad Molecular Imager FX with Quantity One software (BioRad, Veenendaal, The Netherlands). All gene expression analyses were repeated with similar results.

Results

SA-mediated suppression of JA biosynthesis genes

In Arabidopsis, pharmacological experiments revealed that SA can antagonize the expression of JA-responsive genes, such as the marker gene PDF1.2 (Spoel et al. 2003). Figure 1a shows that, while the expression of the SA-responsive marker gene PR-1 (PATHOGENESIS RELATED-1) is upregulated, MeJA-induced expression of PDF1.2 is strongly suppressed within 3 h after application of SA. Also when PDF1.2 transcription was activated by the JA precursor LA, or the JAs OPDA, JA, or JA-Ile, the expression level of PDF1.2 was strongly antagonized by SA (Fig. 1c). Moreover, during simultaneous interaction with the SA-inducing biotrophic downy mildew pathogen H. arabidopsidis and the JA-inducing necrotrophic fungus Alternaria brassicicola, JA-responsive PDF1.2 transcription was suppressed, while SA-responsive PR-1 expression was unaffected (Fig. 1b). These results confirm the notion that biological or chemical activation JA-responsive gene expression in Arabidopsis is antagonized by the SA pathway.
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Fig. 1

SA-mediated suppression of the JA-responsive marker gene PDF1.2 in Arabidopsis.a Northern-blot analysis of transcript levels of the SA-responsive PR-1 gene and the JA-responsive PDF1.2 gene in 5-week-old Arabidopsis Col-0 plants that were treated with 0.1 mM MeJA and 24 h later with either water (control) or 1 mM SA. Leaves were harvested at the indicated time points. bPR-1 and PDF1.2 transcript levels in 5-week-old Arabidopsis plants that were first inoculated with the SA-inducing biotroph H. arabidopsidis and 72 h later with the JA-inducing necrotroph Alternaria brassicicola. Leaves were harvested 24 h after Alternaria brassicicola inoculation. cPR-1 and PDF1.2 transcript levels in 5-week-old Arabidopsis plants 24 h after treatment with 0.1 mM of MeJA, LA, OPDA, JA or JA-Ile, and either water or 1 mM SA. Equal loading of RNA samples was checked using a probe for 18S rRNA or by ethidium bromide staining of the RNA gel

To investigate whether JA-responsive expression of the JA biosynthesis genes AOS, LOX2, AOC2, and OPR3 can be antagonized by SA, 5-week-old wild-type Col-0 plants were treated with MeJA, SA or with a combination of both chemicals. Figure 2a shows that SA suppressed the MeJA-induced expression level of AOS as early as 1 h after treatment. Also at later time points AOS gene expression remained suppressed in the combination treatment. For better visualization of the suppressive effect of SA on MeJA-induced AOS expression we quantified the signal intensities of the Northern blot depicted in Fig. 2a using a Phosphor imager (Fig. 2a, right panel). At all time points tested, AOS transcript levels in SA + MeJA-treated plants were 40–50% of that in the MeJA-treated plants. Figure 2b shows that MeJA-induced expression of LOX2, AOC2, and OPR3 was also sensitive to suppression by SA. At 1 and 6 h after MeJA treatment, the level of suppression by SA was not as pronounced as for AOS, but at 24 h after induction the level of suppression was comparable to that observed for AOS. From these results we conclude that the JA biosynthesis genes AOS, LOX2, AOC2, and OPR3 are sensitive to suppression by SA.
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Fig. 2

SA-mediated suppression of the JA biosynthesis genes AOS, LOX2, AOC2, and OPR3. Northern-blot analysis of transcript levels of the JA biosynthesis genes AOS(a) and LOX2, AOC2, and OPR3(b) in 5-week-old Arabidopsis Col-0 plants that were treated at time point 0 h with 1 mM SA, 0.1 mM MeJA, or with a combination of both chemicals. Leaves were harvested at the indicated time points. Equal loading of RNA samples was checked using a probe for 18S rRNA. Signal intensities of the Northern blots in the left panels were quantified using a Phosphor imager (right panels). Transcript levels in the single MeJA treatments were set to 100%. Please note that the quantitative data displayed in the right panels are quantifications of the corresponding signal intensities in the left panels and thus do not contain error bars. The Northern-blot analyses have been repeated with similar results

PDF1.2 gene expression in JA biosynthesis mutants

We reasoned that if the antagonistic effect of SA on JA signaling can be explained by the suppressive effect of SA on the JA biosynthesis pathway, then Arabidopsis genotypes affected in JA production would be impaired in crosstalk between SA and JA signaling. To investigate this, we needed a JA biosynthesis mutant that was fully blocked in JA-resonsive gene expression upon endogenous stimulation of the JA pathway, but displayed wild-type levels of expression upon exogenous application of MeJA. To this end, we first screened previously characterized Arabidopsis mutants and transgenic lines that are impaired in different steps of the JA biosynthesis pathway for their ability to activate PDF1.2 upon MeJA treatment or inoculation with the necrotrophic fungus Alternaria brassicicola. Arabidopsis lines S-12, aos/dde2,AOC::RNAi lines 5 and 16, opr3, aim1, opcl1, jmt, and jar1, which are affected at different steps in the JA biosynthesis pathway (Fig. 3a), showed wild-type levels of PDF1.2 expression upon exogenous application of MeJA (Fig. 3b). Because MeJA treatment bypasses the JA biosynthesis pathway, these results confirm that downstream of JA biosynthesis the JA signaling pathway is fully functional in these genotypes.
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Fig. 3

Characterization of JA-responsive PDF1.2 and VSP2 expression in JA biosynthesis mutants. a JA biosynthesis pathway and corresponding Arabidopsis genotypes that are affected in JA biosynthesis. b–d Northern-blot analysis of PDF1.2 or VSP2 transcript levels in 5-week-old Arabidopsis plants that were treated with 0.1 mM MeJA, inoculated with the necrotrophic fungus Alternaria brassicicola, or infested with the insect herbivore P. rapae. Genotypes in the Ws-0 background (opr3, aim1); genotypes in the Col-0 background (aos/dde2, AOC::RNAi lines 5 and 16, opcl1, jmt, and jar1); genotype in Col-5 background (S-12). Leaf tissue was harvested 24 h after treatment with MeJA, inoculation with Alternaria brassicicola, or infestation with P. rapae. Equal loading of RNA samples was checked using a probe for 18S rRNA (data in b only given for Col-0, but were similar for all other genotypes). Northern-blot analyses have been repeated with similar results

Previously, we demonstrated that Alternaria brassicicola induces high levels of JA in Arabidopsis (De Vos et al. 2005). Upon inoculation with Alternaria brassicicola, PDF1.2 transcripts were readily detectable in wild-type Col-0, Col-5, and Ws-0 plants (Fig. 3b). Although to a lesser extent, the majority of the genotypes affected in JA biosynthesis also activated PDF1.2 upon inoculation with Alternaria brassicicola, indicating that they are either leaky, or still able to produce biologically active oxylipins. In the case of the two AOC::RNAi lines we detected still about 20% JA and OPDA compared to the wild type following wounding of leaves (data not shown). Similarly, JA-responsive gene expression in mutant aim1 may reflect the residual oxylipin formation as detected upon wounding (Delker et al. 2007). The AOS mutant aos/dde2 was the only genotype that was fully blocked in its capacity to express PDF1.2 upon pathogen infection. At the same time, it showed normal levels of PDF1.2 expression after MeJA treatment (Fig. 3b). In a control experiment, the inability of aos/dde2 to activate PDF1.2 upon inoculation with Alternaria brassicicola was confirmed (Fig. 3c). Also the induction of the JA-responsive marker gene VSP2 upon feeding by larvae of the insect herbivore P. rapae (small cabbage white) was blocked in this mutant (Fig. 3d). Hence, mutant aos/dde2 was used to assess whether the antagonistic effect of SA on JA signaling is affected in plants that are unable to produce JAs.

SA antagonizes JA signaling downstream of the JA biosynthesis pathway

To investigate whether down-regulation of the JA biosynthesis pathway is a central target of SA in the suppression of JA signaling, we tested the ability of aos/dde2 to display SA–JA crosstalk. Figure 4a shows that exogenous application of MeJA or SA to wild-type Col-0 and mutant aos/dde2 plants induced similar levels of PDF1.2 and PR-1 transcription, respectively. When applied in combination with MeJA, SA strongly suppressed the expression of PDF1.2 in both Col-0 and aos/dde2. Hence, SA-mediated suppression of MeJA-induced PDF1.2 expression is fully functional in the JA biosynthesis mutant aos/dde2. Moreover, SA was also able to suppress MeJA-induced PDF1.2 transcription in the Arabidopsis genotypes S-12, AOC::RNAi lines 5 and 16, opr3, aim1, opcl1, jmt and jar1, in most cases to similar levels as in their respective wild-types (Fig. 4b). In the negative control mutant npr1, which is blocked in SA-mediated suppression of JA-responsive gene expression, PDF1.2 was not suppressed by SA, confirming previous findings (Spoel et al. 2003; Leon-Reyes et al. 2009). Collectively, these results indicate that SA antagonizes JA signaling via a target downstream of the JA biosynthesis pathway.
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Fig. 4

Antagonistic effect of SA on PDF1.2 expression in JA biosynthesis mutants. a, b Northern-blot analysis of PR-1 and PDF1.2 transcript levels in 5-week-old Arabidopsis plants that were treated with 1 mM SA, 0.1 mM MeJA, or a combination of both chemicals. For details on the JA biosynthesis mutants used, see legend to Fig. 3. Mutant npr1 was used as a negative control for SA–JA cross-talk. Leaf tissue was harvested 24 h after treatment for RNA analysis. Equal loading of RNA samples was checked using a probe for 18S rRNA. c Quantification of the signal intensities of the Northern blots in a and b using a Phosphor imager. PDF1.2 transcript levels in the single MeJA treatments were set to 100%. Please note that the quantitative data displayed in c are quantifications of the corresponding signal intensities in a and b and thus do not contain error bars. The Northern-blot analyses have been repeated with similar results

Although the combination treatment of SA and MeJA consistently led to suppression of MeJA-induced PDF1.2 expression and generally did not affect the level of PR-1 expression, occasionally a synergistic effect on SA-induced PR-1 expression was observed. For instance, the combination treatment displayed in Fig. 4b shows an enhanced level of PR-1 mRNA accumulation in the genotypes S-12, AOC RNAi line 16, and aim1 when compared to the respective single SA treatment. Because these genotypes show an altered oxylipin signature, it is tempting to speculate that this is caused by the hormonal context in which the SA response is triggered. This is in line with previous observations by Mur et al. (2006) who demonstrated that JA can synergistically enhance SA-induced PR-1 gene expression, depending on the respective concentrations of the hormones.

Discussion

Plant immunity is regulated by a complex network of cross-communicating signaling pathways. The plant hormones SA and JA play crucial roles in controlling plant defenses that are triggered after pathogen or insect attack. The SA and JA signaling pathways are often mutually antagonistic, but the outcome of the signal interaction greatly depends on the context in which they are activated (Pieterse et al. 2009). Early studies in tomato demonstrated that exogenous application of SA suppresses the expression of JA biosynthesis genes, suggesting that SA may target the JA biosynthesis pathway to suppress downstream JA signaling (Peña-Cortés et al. 1993). Previously, Spoel et al. (2003) demonstrated that SA-nonaccumulating Arabidopsis NahG plants produced significantly more JA in response to infection by the SA- and JA-inducing pathogen Pseudomonas syringae pv. tomato DC3000, suggesting that in wild-type Arabidopsis plants SA exerts a suppressive effect on the JA biosynthesis pathway. However, in a study on the interaction between Arabidopsis and the SA- and JA-inducing oomycete pathogen Pythium irregulare, no significant effect of endogenous SA accumulation on JA biosynthesis was observed (Adie et al. 2007). In this study, analysis of the JA-responsive JA biosynthesis genes LOX2, AOS, AOC2, and OPR3 revealed that all were down regulated by exogenous application of SA (Fig. 2), confirming our hypothesis that the JA biosynthesis pathway is a potential target of the antagonistic effect of SA on JA signaling in Arabidopsis.

To investigate the significance of the JA biosynthesis pathway as a target in the suppression of JA signaling by SA, we tested the antagonistic effect of SA on the expression of the JA-responsive marker gene PDF1.2 in the background of various JA biosynthesis mutants. Mutant aos/dde2 appeared to be the only genotype tested that was fully blocked in the ability to express the JA-responsive marker genes PDF1.2 and VSP2 in response to endogenous stimulation of the JA biosynthesis pathway by pathogen or insect attack (Fig. 3). Nevertheless, induction of PDF1.2 transcription in aos/dde2 by exogenous application of MeJA could be fully antagonized by SA (Fig. 4). Similar results were obtained for VSP2 (data not shown). Also in the other JA biosynthesis mutants tested, MeJA-induced PDF1.2 transcription was suppressed by SA to levels that were mostly similar as those observed in their respective wild-types. Interestingly, in the opr3 mutant which is completely JA-deficient but can form OPDA (Stintzi et al. 2001; Delker et al. 2007) a similar suppression was found as in the aos/dde2 mutant, which confirms our findings in Fig. 1c that OPDA- and JAs-induced expression of PDF1.2 are similarly sensitive to suppression by SA. This is worthwhile to mention since evidence is accumulating that OPDA and JAs can differentially activate JA-responsive gene expression (Müller and Berger 2009). Collectively, these results indicate that downregulation of the JA biosynthesis pathway is not essential for SA-mediated suppression of JA signaling. Hence, we conclude that the antagonistic effect of SA on JA signaling acts at a target in the JA signaling pathway that functions downstream of JA biosynthesis. This conclusion is supported by the observation that PDF1.2 transcription by exogenous application of saturating doses of MeJA (up to 2 mM) could still be antagonized by SA (Leon-Reyes et al. 2010), which makes it very unlikely that the suppressive effect of SA on JA signaling acts via the inhibition of JA biosynthesis. However, because several JA biosynthesis genes are upregulated upon activation of the JA signaling pathway, it can not be ruled out that suppression of their expression by SA may eventually contribute to the attenuation of the JA response during plant-attacker interactions.

So what are the potential target sites for the SA-mediated suppression of the JA signaling pathway downstream of JA biosynthesis? The SCFCOI1-complex and JAZ repressor proteins are central components in the perception and transcriptional response to JAs. Mechanisms by which SA prevents JA-mediated degradation of JAZ proteins via the proteasome would suppress JA-responsive gene expression, and thus provide a potential explanation for the mode of action of SA–JA crosstalk. Alternatively, SA may activate negative regulators of JA-responsive gene expression that either inactivate or outcompete positive regulators. Currently, we are in the process of investigating these scenarios.

Acknowledgments

We thank Ruth Joosten, Hans Van Pelt and Ientse Van der Sluis (Plant–Microbe Interactions, Utrecht University) for technical assistance. We thank the following colleagues for kindly sending Arabidopsis seeds: Prof. Keller and Dr. Ringli (University of Zürich, Switzerland; aos/dde2-2), Prof. Browse (Washington State University, Pullman, USA; opr3) and Dr. Brinder (University of Wisconsin, Madison, USA; aim1). This work was supported by VICI grant no. 865.04.002 of the Earth and Life Sciences Foundation, which is subsidized by the Netherlands Organization of Scientific Research.

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Authors and Affiliations

  • Antonio Leon-Reyes
    • 1
    • 2
  • Dieuwertje Van der Does
    • 1
  • Elvira S. De Lange
    • 1
  • Carolin Delker
    • 3
  • Claus Wasternack
    • 3
  • Saskia C. M. Van Wees
    • 1
  • Tita Ritsema
    • 1
    • 5
  • Corné M. J. Pieterse
    • 1
    • 4
  1. 1.Plant–Microbe Interactions, Department of Biology, Faculty of ScienceUtrecht UniversityUtrechtThe Netherlands
  2. 2.Universidad San Francisco de Quito (USFQ), Diego de Robles y Vía Interoceánica (Cumbaya)QuitoEcuador
  3. 3.Leibniz Institute of Plant BiochemistryWeinberg 3Germany
  4. 4.Centre for BioSystems GenomicsWageningenThe Netherlands
  5. 5.Amsterdam Molecular TherapeuticsAmsterdamThe Netherlands