Planta

, Volume 232, Issue 3, pp 677–689

Temporal and spatial changes in cell wall composition in developing grains of wheat cv. Hereward

Authors

  • G. A. Toole
    • Institute of Food ResearchNorwich Research Park
  • G. Le Gall
    • Institute of Food ResearchNorwich Research Park
  • I. J. Colquhoun
    • Institute of Food ResearchNorwich Research Park
  • C. Nemeth
    • Rothamsted Research
  • L. Saulnier
    • INRA UR1268 BiopolymersInteractions Assemblies
  • A. Lovegrove
    • Rothamsted Research
  • T. Pellny
    • Rothamsted Research
  • M. D. Wilkinson
    • Rothamsted Research
  • J. Freeman
    • Rothamsted Research
  • R. A. C. Mitchell
    • Rothamsted Research
  • E. N. C. Mills
    • Institute of Food ResearchNorwich Research Park
    • Rothamsted Research
Original Article

DOI: 10.1007/s00425-010-1199-5

Cite this article as:
Toole, G.A., Le Gall, G., Colquhoun, I.J. et al. Planta (2010) 232: 677. doi:10.1007/s00425-010-1199-5

Abstract

A combination of enzyme mapping, FT-IR microscopy and NMR spectroscopy was used to study temporal and spatial aspects of endosperm cell wall synthesis and deposition in developing grain of bread wheat cv. Hereward. This confirmed previous reports that changes in the proportions of the two major groups of cell wall polysaccharides occur, with β-glucan accumulating earlier in development than arabinoxylan. Changes in the structure of the arabinoxylan occurred, with decreased proportions of disubstituted xylose residues and increased proportions of monosubstituted xylose residues. These are likely to result, at least in part, from arabinoxylan restructuring catalysed by enzymes such as arabinoxylan arabinofurano hydrolase and lead to changes in cell wall mechanical properties which may be required to withstand stresses during grain maturation and desiccation.

Keywords

ArabinoxylanCell wallDietary fibreβ-GlucanWheat grain

Abbreviations

Araf

Arabinofuranosyl units

AX

Arabinoxylan

AXOS

Arabinoxylan oligosaccharide

β-glucan

(1–3)(1–4)β-d-Glucan

daa

Days after anthesis

DP3

3-O-β-Cellobiosyl-d-glucose, G3

DP4

3-O-β-Cellotriosyl-d-glucose, G4

EtOH

Ethanol

FPA

Focal plane array

FT-IR

Fourier-transform infra-red

GAX

Glucuronoarabinoxylan

GOS

Glucooligosaccharide

HB-AX

Highly branched AX

HPAEC

High performance anion exchange chromatography

LB-AX

Low branched AX

NMR

Nuclear magnetic resonance

PCA

Principal component analysis

TSP

Sodium 3-(trimethylsilyl)-propionate-d4

WE-AX

Water-extractable AX

X

Xylose

XX

Xylobiose

Xylp

Xylopyranosyl residues

Supplementary material

425_2010_1199_MOESM1_ESM.doc (67 kb)
Supplementary material 1 (DOC 67 kb)
425_2010_1199_MOESM2_ESM.pdf (11 kb)
Supplementary material 2 (PDF 11 kb)

Copyright information

© Springer-Verlag 2010