Planta

, Volume 232, Issue 1, pp 85–93

Metabolic and histopathological alterations of Jatropha mosaic begomovirus-infected Jatropha curcas L. by HR-MAS NMR spectroscopy and magnetic resonance imaging

  • O. P. Sidhu
  • Sanjay Annarao
  • Uday Pathre
  • S. K. Snehi
  • S. K. Raj
  • Raja Roy
  • Rakesh Tuli
  • C. L. Khetrapal
Original Article

DOI: 10.1007/s00425-010-1159-0

Cite this article as:
Sidhu, O.P., Annarao, S., Pathre, U. et al. Planta (2010) 232: 85. doi:10.1007/s00425-010-1159-0

Abstract

Alterations in the anatomical structures, sap translocation and metabolic profiles in Jatropha curcas L. (Euphorbiaceae), infected with Jatropha mosaic virus (JMV) have been investigated using MRI and HR-MAS NMR spectroscopy. The contrast of MRI images distinguishes abnormalities in anatomical structures of infected and healthy stem. The HR-MAS NMR spectroscopic analysis indicated that viral infection significantly affected the plant metabolism. Higher accumulation of TCA cycle intermediates, such as citrate and malate, in JMV-infected plants suggested a higher rate of respiration. The respiration rate was more than twofold as compared to healthy ones. The viral stress also significantly increases the concentrations of alanine, arginine, glutamine, valine, GABA and choline as compared to healthy ones. Microscopic examination revealed severe hyperplasia caused by JMV with a considerable reduction in the size of stem cells. Lower concentration of glucose and sucrose in viral-infected stem tissues indicates decreased translocation of photosynthates from leaves to stem due to hyperplasia caused by JMV. The MR images distinguished stele, cortical and pith regions of JMV-infected and healthy stems. Contrast of T1- and T2-weighted images showed significant differences in the spatial distribution of water, lipids and macromolecules in virus-infected and healthy stem tissues. The results demonstrated the value of MRI and HR-MAS NMR spectroscopy in studying viral infection and metabolic shift in plants. The present methodology may help in better understanding the metabolic alterations during biotic stress in other plant species of agricultural and commercial importance.

Keywords

HR-MAS NMR Jatropha curcas Jatropha mosaic virus Magnetic resonance imaging Metabolic profiles 

Abbreviations

MRI

Magnetic resonance imaging

HR-MAS NMR

High-resolution magic angle spinning nuclear magnetic resonance

JMV

Jatropha mosaic virus

CPMG

Carr-Purcell Meiboom Gill

COSY

Correlated spectroscopy

TCA

Tricarboxylic acid

PCR

Polymerase chain reaction

GABA

γ-Amino butyric acid

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • O. P. Sidhu
    • 1
  • Sanjay Annarao
    • 2
  • Uday Pathre
    • 1
  • S. K. Snehi
    • 1
  • S. K. Raj
    • 1
  • Raja Roy
    • 2
  • Rakesh Tuli
    • 1
  • C. L. Khetrapal
    • 2
  1. 1.National Botanical Research InstituteLucknowIndia
  2. 2.Centre of Biomedical Magnetic ResonanceSanjay Gandhi Post-Graduate Institute of Medical SciencesLucknowIndia

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