Planta

, Volume 231, Issue 6, pp 1459–1473

A tomato bZIP transcription factor, SlAREB, is involved in water deficit and salt stress response

  • Tsai-Hung Hsieh
  • Chia-Wen Li
  • Ruey-Chih Su
  • Chiu-Ping Cheng
  • Sanjaya
  • Yi-Chien Tsai
  • Ming-Tsair Chan
Original Article

DOI: 10.1007/s00425-010-1147-4

Cite this article as:
Hsieh, T., Li, C., Su, R. et al. Planta (2010) 231: 1459. doi:10.1007/s00425-010-1147-4

Abstract

Abiotic stresses such as cold, water deficit, and salt stresses severely reduce crop productivity. Tomato (Solanum lycopersicum) is an important economic crop; however, not much is known about its stress responses. To gain insight into stress-responsive gene regulation in tomato plants, we identified transcription factors from a tomato cDNA microarray. An ABA-responsive element binding protein (AREB) was identified and named SlAREB. In tomato protoplasts, SlAREB transiently transactivated luciferase reporter gene expression driven by AtRD29A (responsive to dehydration) and SlLAP (leucine aminopeptidase) promoters with exogenous ABA application, which was suppressed by the kinase inhibitor staurosporine, indicating that an ABA-dependent post-translational modification is required for the transactivation ability of SlAREB protein. Electrophoretic mobility shift assays showed that the recombinant DNA-binding domain of SlAREB protein is able to bind AtRD29A and SlLAP promoter regions. Constitutively expressed SlAREB increased tolerance to water deficit and high salinity stresses in both Arabidopsis and tomato plants, which maintained PSII and membrane integrities as well as water content in plant bodies. Overproduction of SlAREB in Arabidopsis thaliana and tomato plants regulated stress-related genes AtRD29A, AtCOR47, and SlCI7-like dehydrin under ABA and abiotic stress treatments. Taken together, these results show that SlAREB functions to regulate some stress-responsive genes and that its overproduction improves plant tolerance to water deficit and salt stress.

Keywords

ABA-responsive element binding proteinAbscisic acidSalinityWater deficit

Abbreviations

ABA

Abscisic acid

ABRE

ABA-responsive element

AREB

ABA-responsive element binding protein

SlAREB

Solanum lycopersicum ABA-responsive element binding protein

RD29A

Responsive to dehydration 29A

LAP

Leucine aminopeptidase

CI7

Cold-induced 7

Supplementary material

425_2010_1147_MOESM1_ESM.ppt (109 kb)
Supplementary Figure S1 (PPT 109 kb)
425_2010_1147_MOESM2_ESM.doc (36 kb)
Supplementary data (DOC 36 kb)

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Tsai-Hung Hsieh
    • 1
    • 5
  • Chia-Wen Li
    • 2
  • Ruey-Chih Su
    • 3
  • Chiu-Ping Cheng
    • 4
  • Sanjaya
    • 1
    • 6
  • Yi-Chien Tsai
    • 1
    • 7
  • Ming-Tsair Chan
    • 2
  1. 1.Agricultural Biotechnology Research Center, Academia SinicaTaipeiTaiwan
  2. 2.Academia Sinica, Biotechnology Center in Southern TaiwanTainanTaiwan
  3. 3.Department of Life ScienceFu Jen Catholic UniversityTaipeiTaiwan
  4. 4.Institute of Plant BiologyNational Taiwan UniversityTaipeiTaiwan
  5. 5.Institute of Plant and Microbial Biology, Academia SinicaTaipeiTaiwan
  6. 6.Department of Biochemistry and Molecular BiologyGreat Lakes Bioenergy Research Center, MSUEast LansingUSA
  7. 7.Genomics Research Center, Academia SinicaTaipeiTaiwan