Planta

, Volume 231, Issue 6, pp 1323–1334

BoPMEI1, a pollen-specific pectin methylesterase inhibitor, has an essential role in pollen tube growth

Original Article

DOI: 10.1007/s00425-010-1136-7

Cite this article as:
Zhang, G.Y., Feng, J., Wu, J. et al. Planta (2010) 231: 1323. doi:10.1007/s00425-010-1136-7

Abstract

Pectin methylesterase (PME) is known to have important roles in pollen development and pollen tube growth. As pivotal regulatory factors in PME activity modulation, PME inhibitors (PMEIs) are thought to be key regulators of cell wall stability at the tip of the pollen tube. We report on the cloning and characterization of a novel B. oleracea PMEI gene, BoPMEI1. Heterologously expressed BoPMEI1 showed PMEI activity. RT-PCR studies of different tissues and promoter-GUS fusions confirmed that BoPMEI1 was specifically expressed in mature pollen grains and pollen tubes. Based on in vivo transient assays, we found that BoPMEI1 appears to be largely localized to the plasma membrane. Transgenic Arabidopsis plants expressing antisense BoPMEI1 under the control of the CaMV 35S promoter suppressed the expression of the orthologous gene At1g10770, which led to partial male sterility and decreased seed set by inhibition of pollen tube growth. Our study demonstrates the involvement of BoPMEI1 in pollen tube growth.

Keywords

Broccoli (B. oleracea var. italica)Male sterilityPectin methylesterase inhibitorPollen tube growth

Abbreviations

PME

Pectin methylesterase

PMEI

Pectin methylesterase inhibitor

RT-PCR

Reverse transcriptase polymerase chain reaction

CaMV

35S Cauliflower mosaic virus 35S

GUS

Beta-glucuronidase

cDNA-AFLP

cDNA-amplified fragment length polymorphism

ORF

Open reading frame

kDa

Kilometer Dalton

nt

Nucleotide

aa

Amino acid

IPTG

Isopropyl β-D-1-thiogalactopyranoside

Kan

Kanamycin

GFP

Green-fluorescent protein

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Guo Yu Zhang
    • 1
  • Jing Feng
    • 1
  • Jian Wu
    • 1
  • Xiao Wu Wang
    • 1
  1. 1.Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesBeijingPeople’s Republic of China