Planta

, Volume 231, Issue 4, pp 887–899

Coordinated regulation of anthocyanin biosynthesis in Chinese bayberry (Myrica rubra) fruit by a R2R3 MYB transcription factor

  • Shan-Shan Niu
  • Chang-Jie Xu
  • Wang-Shu Zhang
  • Bo Zhang
  • Xian Li
  • Kui Lin-Wang
  • Ian B. Ferguson
  • Andrew C. Allan
  • Kun-Song Chen
Original Article

DOI: 10.1007/s00425-009-1095-z

Cite this article as:
Niu, S., Xu, C., Zhang, W. et al. Planta (2010) 231: 887. doi:10.1007/s00425-009-1095-z

Abstract

Chinese bayberry (Myrica rubra) is a fruit crop with cultivars producing fruit ranging from white (Shuijing, SJ) to red (Dongkui, DK) and dark red-purple (Biqi, BQ), as a result of different levels of anthocyanin accumulation. Genes encoding the anthocyanin biosynthesis enzymes chalcone synthase, chalcone isomerase, flavanone 3-hydroxylase (F3H), flavonoid 3′-hydroxylase (F3′H), dihydroflavonol 4-reductase (DFR), anthocyanidin synthase (ANS) and UDP-glucose:flavonoid 3-O-glucosyltransferase (UFGT), as well as MrMYB1, a R2R3 MYB transcription factor homologous to known activators of anthocyanin biosynthesis, were isolated from ripe fruit of BQ. Differences in mRNA abundance of MrF3H, MrF3′H, MrDFR1, MrANS and MrUFGT were highly correlated with differential accumulation of anthocyanins between cultivars, suggesting coordinated regulation by transcription factors. The transcript level of MrMYB1 was strongly associated with the anthocyanin content in ripe fruit of the three cultivars, as well as different anthocyanin containing tissues of BQ fruit. Fruit bagging strongly inhibited anthocyanin accumulation in fruit as well as the expression of all anthocyanin biosynthetic genes and MrMYB1. Over-expression of MrMYB1 stimulated both anthocyanin accumulation and activated an Arabidopsis-DFR promoter in tobacco (Nicotiana tabacum). MrMYB1d, an allele with a 1 bp deletion at nucleotide 30 of coding sequence, was observed in SJ and DK fruit, suggesting that a nonsense mutation of the MYB1 protein may be responsible for no or low expression of MYB1 in the white and red fruit. These results show that coordinated expression of multiple biosynthetic genes is involved in anthocyanin accumulation in Chinese bayberry fruit, and this is regulated by MrMYB1.

Keywords

Anthocyanin biosynthesisChinese bayberryCoordinated gene expressionNonsense mutationMYBTranscription factor

Abbreviations

ANS

Anthocyanidin synthase

bHLH

Basic-helix-loop-helix

BQ

Biqi

CDS

Coding sequence

CHI

Chalcone isomerase

CHS

Chalcone synthase

DAFB

Days after full bloom

DFR

Dihydroflavonol 4-reductase

DK

Dongkui

EBGs

Early biosynthetic genes

F3H

Flavanone 3-hydroxylase

F3′H

Flavonoid 3′-hydroxylase

LBGs

Late biosynthetic genes

LUC

Firefly luciferase

Q-PCR

Real-time quantitative PCR

REN

Renilla luciferase

SJ

Shuijing

TFs

Transcription factors

UFGT

UDP-glucose:flavonoid 3-O-glucosyltransferase

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Shan-Shan Niu
    • 1
  • Chang-Jie Xu
    • 1
  • Wang-Shu Zhang
    • 1
  • Bo Zhang
    • 1
  • Xian Li
    • 1
  • Kui Lin-Wang
    • 2
  • Ian B. Ferguson
    • 2
  • Andrew C. Allan
    • 2
  • Kun-Song Chen
    • 1
  1. 1.Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality ImprovementZhejiang UniversityHangzhouPeople’s Republic of China
  2. 2.The New Zealand Institute of Plant and Food ResearchAucklandNew Zealand