Planta

, Volume 231, Issue 3, pp 705–716

miR398 and miR408 are up-regulated in response to water deficit in Medicago truncatula

  • Inês Trindade
  • Cláudio Capitão
  • Tamas Dalmay
  • Manuel Pedro Fevereiro
  • Dulce Metelo dos Santos
Original Article

DOI: 10.1007/s00425-009-1078-0

Cite this article as:
Trindade, I., Capitão, C., Dalmay, T. et al. Planta (2010) 231: 705. doi:10.1007/s00425-009-1078-0

Abstract

Plant microRNAs have been implicated in various abiotic stress responses. We identified several conserved microRNAs that showed differential expression in Medicago truncatula plants subjected to water deficit: miR169 is down-regulated only in the roots and miR398a/b and miR408 are strongly up-regulated in both shoots and roots. Down-regulation of miR169 in the roots did not correlate with accumulation of its target MtHAP2-1 transcripts, suggesting that its regulation may not occur at the mRNA level or may depend on other regulatory mechanisms, which do not involve this miRNA, in water-deficit conditions. The up-regulation of miR398a/b and miR408 and the clear down-regulation of their respective target genes, which encode the copper proteins COX5b (subunit 5b of mitochondrial cytochrome c oxidase) and plantacyanin, highlight the involvement of these miRNAs in response to water deprivation in M. truncatula. Also, miR398 up-regulation is inversely correlated with the down-regulation of copper superoxide dismutase, CSD1, during water deficit. The regulation of genes encoding copper proteins by miR398a/b and miR408 suggests a link between copper homeostasis and M. truncatula adaptation to progressive water deficit.

Keywords

Differential expressionMedicagoMicroRNA398MicroRNA408Water deficit

Abbreviations

COX5b

Subunit 5b of mitochondrial cytochrome c oxidase

CSD

Copper superoxide dismutase

HDT3

Histone deacetylase

MiRNA

MicroRNA

P1B-ATPase

P1B-Adenosine triphosphatase

5′-RACE

5′-Rapid amplification of cDNA ends

UTR

Untranslated region

Supplementary material

425_2009_1078_MOESM1_ESM.tif (5.3 mb)
Supplemental Fig. S1 (TIF 5.25 mb)
425_2009_1078_MOESM2_ESM.doc (40 kb)
Supplemental Table S1 (DOC 39.5 kb)
425_2009_1078_MOESM3_ESM.doc (48 kb)
Supplemental Table S2 (DOC 48 kb)

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Inês Trindade
    • 1
  • Cláudio Capitão
    • 1
  • Tamas Dalmay
    • 2
  • Manuel Pedro Fevereiro
    • 1
    • 3
  • Dulce Metelo dos Santos
    • 1
    • 4
  1. 1.Laboratório de Biotecnologia de Células Vegetais, Instituto de Tecnologia Química e BiológicaUniversidade Nova de LisboaOeirasPortugal
  2. 2.School of Biological SciencesUniversity of East AngliaNorwichUK
  3. 3.Departamento de Biologia Vegetal, Faculdade de CiênciasUniversidade de LisboaLisbonPortugal
  4. 4.Centro de Veterinária e Zootecnia, Instituto de Investigação Científica e TropicalUniversidade Técnica de LisboaLisbonPortugal