Planta

, Volume 230, Issue 5, pp 1047–1055

Susceptibility of Phelipanche and Orobanche species to AAL-toxin

  • Axel de Zélicourt
  • Grégory Montiel
  • Jean-Bernard Pouvreau
  • Séverine Thoiron
  • Sabine Delgrange
  • Philippe Simier
  • Philippe Delavault
Original Article

DOI: 10.1007/s00425-009-1008-1

Cite this article as:
de Zélicourt, A., Montiel, G., Pouvreau, JB. et al. Planta (2009) 230: 1047. doi:10.1007/s00425-009-1008-1

Abstract

Fusarium and Alternaria spp. are phytopathogenic fungi which are known to be virulent on broomrapes and to produce sphinganine-analog mycotoxins (SAMs). AAL-toxin is a SAM produced by Alternaria alternata which causes the inhibition of sphinganine N-acyltransferase, a key enzyme in sphingolipid biosynthesis, leading to accumulation of sphingoid bases. These long chain bases (LCBs) are determinant in the occurrence of programmed cell death (PCD) in susceptible plants. We showed that broomrapes are sensitive to AAL-toxin, which is not common plant behavior, and that AAL-toxin triggers cell death at the apex of the radicle as well as LCB accumulation and DNA laddering. We also demonstrated that three Lag1 homologs, encoding components of sphinganine N-acyltransferase in yeast, are present in the Orobanche cumana genome and two of them are mutated leading to an enhanced susceptibility to AAL-toxin. We therefore propose a model for the molecular mechanism governing broomrape susceptibility to the fungus Alternaria alternata.

Keywords

Alternaria alternata toxin (AAL-toxin)BiocontrolLongevity assurance geneParasitic plantProgrammed cell deathSphinganine-N-acyl transferase

Abbreviations

3-KDHS

3-Ketodihydrosphingosine

AAL-toxin

Alternaria alternata toxin

Asc

Alternaria stem canker

DHS

Dihydrosphingosine = Sphinganine

Lag

Longevity assurance gene

LCB

Long chain base

PCD

Programmed cell death

PHS

Phytosphingosine

SAM

Sphinganine-analog mycotoxin

So

Sphingosine

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Axel de Zélicourt
    • 1
    • 2
    • 3
  • Grégory Montiel
    • 1
  • Jean-Bernard Pouvreau
    • 1
  • Séverine Thoiron
    • 1
  • Sabine Delgrange
    • 1
  • Philippe Simier
    • 1
  • Philippe Delavault
    • 1
  1. 1.Laboratoire de Biologie et Pathologie Végétales, EA 1157, IFR 149 QUASAVUniversité de NantesNantes cedex 3France
  2. 2.Institut des Sciences du VégétalC.N.R.SGif-sur-YvetteFrance
  3. 3.Université Paris Diderot Paris 7, Les Grands MoulinsParis Cedex 13France