Planta

, Volume 230, Issue 1, pp 149–163

Genome-wide identification of BURP domain-containing genes in rice reveals a gene family with diverse structures and responses to abiotic stresses

Authors

  • Xipeng Ding
    • National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan)Huazhong Agricultural University
  • Xin Hou
    • National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan)Huazhong Agricultural University
  • Kabin Xie
    • National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan)Huazhong Agricultural University
    • National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan)Huazhong Agricultural University
    • National Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural University
Original Article

DOI: 10.1007/s00425-009-0929-z

Cite this article as:
Ding, X., Hou, X., Xie, K. et al. Planta (2009) 230: 149. doi:10.1007/s00425-009-0929-z

Abstract

Increasing evidence suggests that a gene family encoding proteins containing BURP domains have diverse functions in plants, but systematic characterization of this gene family have not been reported. In this study, 17 BURP family genes (OsBURP0117) were identified and analyzed in rice (Oryza sativa L.). These genes have diverse exon–intron structures and distinct organization of putative motifs. Based on the phylogenetic analysis of BURP protein sequences from rice and other plant species, the BURP family was classified into seven subfamilies, including two subfamilies (BURP V and BURP VI) with members from rice only and one subfamily (BURP VII) with members from monocotyledons only. Two BURP gene clusters, belonging to BURP V and BURP VI, were located in the duplicated region on chromosome 5 and 6 of rice, respectively. Transcript level analysis of BURP genes of rice in various tissues and organs revealed different tempo-spatial expression patterns, suggesting that these genes may function at different stages of plant growth and development. Interestingly, all the genes of the BURP VII subfamily were predominantly expressed in flower organs. We also investigated the expression patterns of BURP genes of rice under different stress conditions. The results suggested that, except for two genes (OsBURP01 and OsBURP13), all other members were induced by at least one of the stresses including drought, salt, cold, and abscisic acid treatment. Two genes (OsBURP05 and OsBURP16) were responsive to all the stress treatments and most of the OsBURP genes were responsive to salt stress. Promoter sequence analysis revealed an over-abundance of stress-related cis-elements in the stress-responsive genes. The data presented here provide important clues for elucidating the functions of genes of this family.

Keywords

Abiotic stress BURP domain Oryza RD22 Transcript level

Abbreviations

ABA

Abscisic acid

ABRE

ABA responsive element

BURP

BNM2, USP, RD22, and PG1β

DRE

Dehydration-responsive element

LTRE

Low temperature-responsive element

Supplementary material

425_2009_929_MOESM1_ESM.doc (51 kb)
Supplementary material 1 (DOC 51 kb)

Copyright information

© Springer-Verlag 2009