Planta

, Volume 229, Issue 5, pp 1077–1086

Identification of a Polygonum cuspidatum three-intron gene encoding a type III polyketide synthase producing both naringenin and p-hydroxybenzalacetone

  • Lan-Qing Ma
  • Yan-Wu Guo
  • Dong-Yao Gao
  • Dong-Ming Ma
  • You-Nian Wang
  • Guo-Feng Li
  • Ben-Ye Liu
  • Hong Wang
  • He-Chun Ye
Original Article

DOI: 10.1007/s00425-009-0899-1

Cite this article as:
Ma, L., Guo, Y., Gao, D. et al. Planta (2009) 229: 1077. doi:10.1007/s00425-009-0899-1

Abstract

Benzalacetone synthase (BAS) is a member of the plant-specific type III PKS superfamily that catalyzes a one-step decarboxylative condensation of 4-coumaroyl-CoA with malonyl-CoA to produce p-hydroxybenzalacetone. In our recent work (Ma et al. in Planta 229(3):457–469, 2008), a three-intron type III PKS gene (PcPKS2) was isolated from Polygonum cuspidatum Sieb. et Zucc. Phylogenetic and functional analyses revealed this recombinant PcPKS2 to be a BAS. In this study, another three-intron type III PKS gene (PcPKS1) and its corresponding cDNA were isolated from P. cuspidatum. Sequence and phylogenetic analyses demonstrated that PcPKS1 is a chalcone sythase (CHS). However, functional and enzymatic analyses showed that recombinant PcPKS1 is a bifunctional enzyme with both, CHS and BAS activity. DNA gel blot analysis indicated that there are two to four CHS copies in the P. cuspidatum genome. RNA gel blot analysis revealed that PcPKS1 is highly expressed in the rhizomes and in young leaves, but not in the roots of the plant. PcPKS1 transcripts in leaves were inducible by pathogen infection and wounding. BAS is thought to play a crucial role in the construction of the C6–C4 moiety found in a variety of phenylbutanoids, yet so far phenylbutanoids have not been isolated from P. cuspidatum. However, since PcPKS1 and PcPKS2 (Ma et al. in Planta 229(3):457–469, 2008) have been identified in P. cuspidatum, it is possible that such compounds are also produced in that plant, albeit in low concentrations.

Keywords

Benzalacetone synthaseChalcone synthaseGene evolutionPhenylbutanoid biosynthesisPolygonumType III polyketide synthase

Abbreviations

ALS

Aloesone synthase

BAS

Benzalacetone synthase

CHS

Chalcone synthase

CoA

Coenzyme A

LC-ESIMS

Liquid chromatography-electron spray ionization mass spectrometry

OKS

Octaketide synthase

PKS

Polyketide synthase

STS

Stilbene synthase

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Lan-Qing Ma
    • 1
  • Yan-Wu Guo
    • 1
  • Dong-Yao Gao
    • 2
  • Dong-Ming Ma
    • 1
  • You-Nian Wang
    • 2
  • Guo-Feng Li
    • 1
  • Ben-Ye Liu
    • 1
  • Hong Wang
    • 1
  • He-Chun Ye
    • 1
  1. 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of BotanyChinese Academy of SciencesBeijingPeople’s Republic of China
  2. 2.Key Laboratory of New Technology in Agricultural Application of BeijingBeijing University of AgricultureBeijingPeople’s Republic of China