Planta

, Volume 224, Issue 6, pp 1449–1464

Fungal and plant gene expression during the colonization of cacao seedlings by endophytic isolates of four Trichoderma species

  • B. A. Bailey
  • H. Bae
  • M. D. Strem
  • D. P. Roberts
  • S. E. Thomas
  • J. Crozier
  • G. J. Samuels
  • Ik-Young Choi
  • K. A. Holmes
Original Article

DOI: 10.1007/s00425-006-0314-0

Cite this article as:
Bailey, B.A., Bae, H., Strem, M.D. et al. Planta (2006) 224: 1449. doi:10.1007/s00425-006-0314-0

Abstract

Endophytic isolates of Trichoderma species are being considered as biocontrol agents for diseases of Theobroma cacao (cacao). Gene expression was studied during the interaction between cacao seedlings and four endophytic Trichoderma isolates, T. ovalisporum-DIS 70a, T. hamatum-DIS 219b, T. harzianum-DIS 219f, and Trichoderma sp.-DIS 172ai. Isolates DIS 70a, DIS 219b, and DIS 219f were mycoparasitic on the pathogen Moniliophthora roreri, and DIS 172ai produced metabolites that inhibited growth of M. roreri in culture. ESTs (116) responsive to endophytic colonization of cacao were identified using differential display and their expression analyzed using macroarrays. Nineteen cacao ESTs and 17 Trichoderma ESTs were chosen for real-time quantitative PCR analysis. Seven cacao ESTs were induced during colonization by the Trichoderma isolates. These included putative genes for ornithine decarboxylase (P1), GST-like proteins (P4), zinc finger protein (P13), wound-induced protein (P26), EF-calcium-binding protein (P29), carbohydrate oxidase (P59), and an unknown protein (U4). Two plant ESTs, extensin-like protein (P12) and major intrinsic protein (P31), were repressed due to colonization. The plant gene expression profile was dependent on the Trichoderma isolate colonizing the cacao seedling. The fungal ESTs induced in colonized cacao seedlings also varied with the Trichoderma isolate used. The most highly induced fungal ESTs were putative glucosyl hydrolase family 2 (F3), glucosyl hydrolase family 7 (F7), serine protease (F11), and alcohol oxidase (F19). The pattern of altered gene expression suggests a complex system of genetic cross talk occurs between the cacao tree and Trichoderma isolates during the establishment of the endophytic association.

Keywords

AntibiosisEndophyteMycoparasitisimTheobroma cacaoTrichoderma

Supplementary material

425_2006_314_MOESM1_ESM.doc (28 kb)
Supplementary material
425_2006_314_MOESM2_ESM.xls (336 kb)
Supplementary material

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • B. A. Bailey
    • 1
  • H. Bae
    • 1
  • M. D. Strem
    • 1
  • D. P. Roberts
    • 2
  • S. E. Thomas
    • 3
  • J. Crozier
    • 3
  • G. J. Samuels
    • 4
  • Ik-Young Choi
    • 5
  • K. A. Holmes
    • 3
  1. 1.Sustainable Perennial Crops LaboratoryBeltsvilleUSA
  2. 2.Sustainable Agricultural Systems LaboratoryBeltsvilleUSA
  3. 3.CABI, UK Centre (Ascot)BerksUK
  4. 4.Systematic Botany and Mycology LaboratoryBeltsvilleUSA
  5. 5.Soybean Genomics and Improvement LaboratoryBeltsvilleUSA