Planta

, Volume 224, Issue 4, pp 812–827

Relationship between DNA methylation and histone acetylation levels, cell redox and cell differentiation states in sugarbeet lines

  • Adisa Causevic
  • Marie-Véronique Gentil
  • Alain Delaunay
  • Walid Abu El-Soud
  • Zacarias Garcia
  • Christophe Pannetier
  • Franck Brignolas
  • Daniel Hagège
  • Stéphane Maury
Original Article

DOI: 10.1007/s00425-006-0267-3

Cite this article as:
Causevic, A., Gentil, MV., Delaunay, A. et al. Planta (2006) 224: 812. doi:10.1007/s00425-006-0267-3

Abstract

In order to evaluate the permanent chromatin remodeling in plant allowing their high developmental plasticity, three sugarbeet cell lines (Beta vulgaris L. altissima) originating from the same mother plant and exhibiting graduate states of differentiation were analyzed. Cell differentiation has been estimated by the cell redox state characterized by 36 biochemical parameters as reactive oxygen species steady-state levels, peroxidation product contents and enzymatic or non-enzymatic protective systems. Chromatin remodeling has been estimated by the measurement of levels of DNA methylation, histone acetylation and corresponding enzyme activities that were shown to differ between cell lines. Furthermore, distinct loci related to proteins involved in cell cycle, gene expression regulation and cell redox state were shown by restriction landmark genome scanning or bisulfite sequencing to display differential methylation states in relation to the morphogenic capacity of the lines. DNA methylating, demethylating and/or histone acetylating treatments allowed to generate a collection of sugarbeet cell lines differing by their phenotypes (from organogenic to dedifferentiated), methylcytosine percentages (from 15.0 to 43.5%) and acetylated histone ratios (from 0.37 to 0.52). Correlations between methylcytosine or acetylated histone contents and levels of various parameters (23 or 7, respectively, out of 36) of the cell redox state could be established. These data lead to the identification of biomarkers of sugarbeet morphogenesis in vitro under epigenetic regulation and provide evidence for a connection between plant morphogenesis in vitro, cell redox state and epigenetic mechanisms.

Keywords

AntioxidantHistone acetylationMethylcytosineMorphogenesisEpigeneticSugarbeet (Beta vulgaris L. altissima)

Abbreviations

APX

Ascorbate peroxidase

CAT

Catalase

DD

Dedifferentiated callus line

DHAR

Dehydroascorbate reductase

DNMT

DNA methyltransferase

GR

Glutathione reductase

GSH

Reduced glutathione

GSSG

Oxidized glutathione

HDAC

Histone deacetylase

HPLC

High-performance liquid chromatography

LC–MS

Liquid chromatography–mass spectrometry

mC

Methylcytosine

MDAR

Monodehydroascorbate reductase

NEAOP

Non-enzymatic antioxidant properties

NO

Non-organogenic callus line

O

Organogenic callus line

PCA

Perchloric acid

PMSF

Phenylmethanesulfonyl fluoride

RLGS

Restriction landmark genome scanning

ROS

Reactive oxygen species

SE

Standard error

SOD

Superoxide dismutase

TBARs

Thiobarbituric acid reactive substances

Supplementary material

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Adisa Causevic
    • 1
    • 2
    • 3
  • Marie-Véronique Gentil
    • 1
  • Alain Delaunay
    • 1
  • Walid Abu El-Soud
    • 4
  • Zacarias Garcia
    • 5
  • Christophe Pannetier
    • 5
  • Franck Brignolas
    • 1
  • Daniel Hagège
    • 1
  • Stéphane Maury
    • 1
  1. 1.Laboratoire de Biologie des Ligneux et des Grandes Cultures, Faculté des sciencesUniversité d’OrléansOrléans Cedex 2France
  2. 2.Department of BiologyUniversity of SarajevoSarajevoBosnia-Herzegovina
  3. 3.Institute for Genetic Engineering and BiotechnologySarajevoBosnia-Herzegovina
  4. 4.Division of Molecular Biology, BiocenterInnsbruck Medical UniversityInnsbruckAustria
  5. 5.Institut PasteurParis Cedex 15France