Planta

, Volume 221, Issue 5, pp 705–715

Low temperature promotes intron retention in two e-cor genes of durum wheat

  • Anna Maria Mastrangelo
  • Sara Belloni
  • Samantha Barilli
  • Benedetto Ruperti
  • Natale Di Fonzo
  • Antonio Michele Stanca
  • Luigi Cattivelli
Original Article

DOI: 10.1007/s00425-004-1475-3

Cite this article as:
Mastrangelo, A.M., Belloni, S., Barilli, S. et al. Planta (2005) 221: 705. doi:10.1007/s00425-004-1475-3

Abstract

Following the screening of a suppression subtractive library developed from durum wheat plants exposed to low temperature for 6 h, two early cold-regulated (e-cor) genes have been isolated. These genes, coding putatively for a ribokinase (7H8) and a C3H2C3 RING-finger protein (6G2), were characterized by the stress-induced retention of a subset of introns in the mature mRNA. This feature was dependent on cold for 7H8 and on cold and dehydration for 6G2. When other genes, such as the stress-related gene WCOR410c, coding for a dehydrin (one intron), or a gene coding for a putative ATP binding cassette transporter (16 introns) were analyzed, no cold-dependent intron retention was observed. Cold-induced intron retention was not observed in mutants defective in the chloroplast development; nevertheless treatment with cycloheximide in the absence of cold was able to promote intron retention for the 7H8 e-cor gene. These results suggest that the cold-induced intron retention reflects the response of the spliceosoma to specific environmental signals transduced to the splicing protein factors through a chloroplast-dependent pathway. Notably, when the 7H8 Arabidopsis orthologous gene was analyzed, no stress induction in terms of mRNA abundance and no cold-dependent intron retention was detected. Otherwise, 6G2 Arabidopsis homologous sequences sharing the same genomic structure of the durum wheat 6G2 showed a similar intron retention event although not strictly dependent on stress.

Keywords

Abiotic stressArabidopsisDurum wheatIntron retentionRibokinaseRING-finger protein

Abbreviations

ATMEKK

Arabidopsis thaliana MAP kinase or ERK kinase kinase

CBF/DREB1

C-repeat binding factor/dehydration responsive element binding protein

RWC

Relative water content

RPL12

Ribosomal protein L12

e-cor

Early cold-regulated

Pfam

Protein families database

SMART

Simple modular architecture research tool

RING

Really interesting gene

SUMO

Small ubiquitin-related modifier

CHX

Cycloheximide

dhn8

Dehydrin 8

WCOR410c

Wheat cold-regulated gene 410c

ABC

ATP binding cassette

Supplementary material

425_2004_1475_ESM_supp.pdf (24 kb)
PDF (25 KB)

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Anna Maria Mastrangelo
    • 1
  • Sara Belloni
    • 2
  • Samantha Barilli
    • 2
  • Benedetto Ruperti
    • 3
  • Natale Di Fonzo
    • 1
  • Antonio Michele Stanca
    • 2
  • Luigi Cattivelli
    • 2
  1. 1.Experimental Institute for Cereal ResearchFoggiaItaly
  2. 2.Experimental Institute for Cereal ResearchFiorenzuola D’Arda (PC)Italy
  3. 3.Department of Crop Production and Agricultural TechnologiesUdine (UD)Italy
  4. 4.Experimental Institute for Cereal ResearchFoggiaItaly