Planta

, Volume 221, Issue 3, pp 394–405

The Arabidopsis SERK1 protein interacts with the AAA-ATPase AtCDC48, the 14-3-3 protein GF14λ and the PP2C phosphatase KAPP

  • Ingrid M. Rienties
  • Josefien Vink
  • Jan Willem Borst
  • Eugenia Russinova
  • Sacco C. de Vries
Original Article

DOI: 10.1007/s00425-004-1447-7

Cite this article as:
Rienties, I.M., Vink, J., Borst, J.W. et al. Planta (2005) 221: 394. doi:10.1007/s00425-004-1447-7

Abstract

Leucine-rich repeat (LRR)-containing transmembrane receptor-like kinases (RLKs) are important components of plant signal transduction. The Arabidopsis thaliana somatic embryogenesis receptor-like kinase 1 (AtSERK1) is an LRR-RLK proposed to participate in a signal transduction cascade involved in embryo development. By yeast two-hybrid screening we identified AtCDC48, a homologue of the mammalian AAA-ATPase p97 and GF14λ, a member of the Arabidopsis family of 14-3-3 proteins as AtSERK1 interactors. In vitro, the AtSERK1 kinase domain is able to transphosphorylate and bind both AtCDC48 and GF14λ. In yeast, AtCDC48 interacts with GF14λ and with the PP2C phosphatase KAPP. In plant protoplasts AtSERK1 interacts with GF14λ.

Keywords

14-3-3 ProteinArabidopsisCell division cycle protein 48Kinase associated protein phosphatase (KAPP)Signal transductionSomatic embryogenesis receptor-like kinase 1

Abbreviations

RLK

Receptor-like kinase

LRR

Leucine-rich repeat

MBP

Maltose binding protein

GST

Glutathione-S-transferase

FRET

Förster resonance energy transfer

FSPIM

Fluorescence spectral imaging microscopy

APB

Acceptor photobleaching

CFP

Cyan fluorescent protein

YFP

Yellow fluorescent protein

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Ingrid M. Rienties
    • 1
  • Josefien Vink
    • 1
  • Jan Willem Borst
    • 2
  • Eugenia Russinova
    • 1
  • Sacco C. de Vries
    • 1
  1. 1.Laboratory of Biochemistry, Department of Agrotechnology and Food SciencesWageningen University and Research CentreWageningenThe Netherlands
  2. 2.MicroSpectroscopy CentreWageningen University and Research CentreWageningenthe Netherlands