Planta

, Volume 220, Issue 5, pp 645–657

Molecular analysis of the CRINKLY4 gene family in Arabidopsis thaliana

  • Xueyuan Cao
  • Kejian Li
  • Sang-Gon Suh
  • Tao Guo
  • Philip W. Becraft
Original Article

DOI: 10.1007/s00425-004-1378-3

Cite this article as:
Cao, X., Li, K., Suh, S. et al. Planta (2005) 220: 645. doi:10.1007/s00425-004-1378-3

Abstract

The maize (Zea mays L.) CRINKLY4 (CR4) gene encodes a serine/threonine receptor-like kinase that controls an array of developmental processes in the plant and endosperm. The Arabidopsis thaliana (L.) Heynh. genome encodes an ortholog of CR4, ACR4, and four CRINKLY4-RELATED (CRR) proteins: AtCRR1, AtCRR2, AtCRR3 and AtCRK1. The available genome sequence of rice (Oryza sativa L.) encodes a CR4 ortholog, OsCR4, and four CRR proteins: OsCRR1, OsCRR2, OsCRR3 and OsCRR4, not necessarily orthologous to the Arabidopsis CRRs. A phylogenetic study showed that AtCRR1 and AtCRR2 form a clade closest to the CR4 group while all the other CRRs form a separate cluster. The five Arabidopsis genes are differentially expressed in various tissues. A construct formed by fusion of the ACR4 promoter and the GUS reporter, ACR4::GUS, is expressed primarily in developing tissues of the shoot. The ACR4 cytoplasmic domain functions in vitro as a serine/threonine kinase, while the AtCRR1 and AtCRR2 kinases are not active. The ability of ACR4 to phosphorylate AtCRR2 suggests that they might function in the same signal transduction pathway. T-DNA insertions were obtained in ACR4, AtCRR1, AtCRR2, AtCRR3 and AtCRK1. Mutations in acr4 show a phenotype restricted to the integuments and seed coat, suggesting that Arabidopsis might contain a redundant function that is lacking in maize. The lack of obvious mutant phenotypes in the crr mutants indicates they are not required for the hypothetical redundant function.

Keywords

ArabidopsisCRINKLY4EvolutionOryzaReceptor-like kinaseSignal transduction

Abbreviations

CR4

CRINKLY4

CRR

CRINKLY4-RELATED

GST

Glutathione S-transferase

GUS

β-Glucuronidase

RCC1

Regulator of chromosome condensation 1

RLK

Receptor-like kinase

SAM

Shoot apical meristem

TNFR

Tumor necrosis factor receptor

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Xueyuan Cao
    • 1
    • 3
  • Kejian Li
    • 1
    • 3
  • Sang-Gon Suh
    • 1
    • 5
  • Tao Guo
    • 1
    • 4
  • Philip W. Becraft
    • 1
    • 2
    • 3
  1. 1.Department of Genetics, Development and Cell BiologyIowa State UniversityAmesUSA
  2. 2.Department of AgronomyIowa State UniversityAmesUSA
  3. 3.Molecular, Cellular, and Developmental Biology ProgramIowa State UniversityAmesUSA
  4. 4.Interdepartmental Genetics ProgramIowa State UniversityAmesUSA
  5. 5.Department of HorticultureYeungnam UniversityKyongsanRepublic of Korea