Planta

, Volume 220, Issue 2, pp 262–270

The ethylene-, jasmonate-, abscisic acid- and NaCl-responsive tomato transcription factor JERF1 modulates expression of GCC box-containing genes and salt tolerance in tobacco

  • Haiwen Zhang
  • Zejun Huang
  • Bingyan Xie
  • Qi Chen
  • Xin Tian
  • Xiulin Zhang
  • Hongbo Zhang
  • Xiangyang Lu
  • Dafang Huang
  • Rongfeng Huang
Original Article

DOI: 10.1007/s00425-004-1347-x

Cite this article as:
Zhang, H., Huang, Z., Xie, B. et al. Planta (2004) 220: 262. doi:10.1007/s00425-004-1347-x

Abstract

Ethylene responsive factors (ERFs) are important plant-specific transcription factors, some of which have been demonstrated to interact with the ethylene-responsive GCC box and the dehydration-responsive element (DRE); however, data on the roles of ERF proteins in connection with various signaling pathways are limited. In this research, we used the GCC box, an essential cis-acting element responsive to ethylene and methyl jasmonate (MeJA), as bait in a yeast one-hybrid system to isolate transcription factors from tomato (Lycopersicon esculentum Mill.). One of the cDNAs, which was designated Jasmonate and Ethylene Response Factor 1 (JERF1), encodes an ERF protein, containing a conserved ERF DNA-binding motif and functioning as a transcriptional activator in yeast through targeting to the nucleus in onion (Allium cepa L.) epidermal cells. Biochemical analysis revealed that JERF1 bound not only to the GCC box but also to the DRE sequence. Expression of the JERF1 gene in tomato was induced by ethylene, MeJA, abscisic acid (ABA) and salt treatment, indicating that JERF1 might act as a connector among different signal transduction pathways. Further research with transgenic JERF1 tobacco (Nicotiana tabacum L.) plants indicated that overexpressing JERF1 activated expression of GCC box-containing genes such as osmotin, GLA, Prb-1b and CHN50 under normal growth conditions, and subsequently resulted in enhanced tolerance to salt stress, suggesting that JERF1 modulates osmotic tolerance by activation of downstream gene expression through interaction with the GCC box or DRE.

Keywords

Abscisic acidDehydration-responsive elementEthylene-responsive GCC boxJasmonateTomato transcription factor JERF1Salt tolerance

Abbreviations

ABA

Abscisic acid

CaMV

Cauliflower mosaic virus

DRE

Dehydration-responsive element

ERF

Ethylene-responsive factor

GFP

Green fluorescent protein

JA

Jasmonate

JERF1

Jasmonate and ethylene responsive factor 1

MeJA

Methyl jasmonic acid

NLS

Nuclear localization signal

OE

Overexpression of JERF1 in tobacco

PR

Pathogenesis-related

WT

Wild type

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Haiwen Zhang
    • 1
    • 2
    • 3
  • Zejun Huang
    • 1
  • Bingyan Xie
    • 2
  • Qi Chen
    • 1
  • Xin Tian
    • 2
  • Xiulin Zhang
    • 1
  • Hongbo Zhang
    • 1
  • Xiangyang Lu
    • 3
  • Dafang Huang
    • 1
  • Rongfeng Huang
    • 1
  1. 1.The National Plant Gene Research Center (Beijing), Biotechnology Research InstituteChinese Academy of Agricultural SciencesBeijingChina
  2. 2.Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesBeijingChina
  3. 3.Hunan Agricultural UniversityChangshaChina