Planta

, Volume 219, Issue 3, pp 459–467

Organic and inorganic nitrogen uptake in lichens

  • Lena Dahlman
  • Jörgen Persson
  • Kristin Palmqvist
  • Torgny Näsholm
Original Article

DOI: 10.1007/s00425-004-1247-0

Cite this article as:
Dahlman, L., Persson, J., Palmqvist, K. et al. Planta (2004) 219: 459. doi:10.1007/s00425-004-1247-0
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Abstract

In order to learn more about nitrogen (N) acquisition in lichens, and to see whether different lichens differ in their affinity to various N sources, N uptake was measured in 14 various lichen associations (“species”). These species represented various morphologies (fruticose or foliose), contrasting microhabitat preferences (epiphytic or terricolous), and had green algal, cyanobacterial or both forms of photobionts. N was supplied under non-limiting conditions as an amino acid mixture, ammonium, or nitrate, using 15N to quantify uptake. Carbonyl cyanide m-chlorophenylhydrazone (CCCP) was used to separate active and passive uptake. Thallus N, amino acids, soluble polyol concentrations, and the biont-specific markers chlorophyll a and ergosterol were quantified, aiming to test if these metabolites or markers were correlated with N uptake capacity. Ammonium uptake was significantly greater and to a higher extent passive, relative to the other two N sources. Nitrate uptake differed among lichen photobiont groups, cyanobacterial lichens having a lower uptake rate. All lichens had the capacity to assimilate amino acids, in many species at rates equal to nitrate uptake or even higher, suggesting that organic N compounds could potentially have an important role in the N nutrition of these organisms. There were no clear correlations between N uptake rates and any of the measured metabolites or markers. The relative uptake rates of ammonium, nitrate and amino acids were not related to morphology or microhabitat.

Keywords

Amino acidAmmoniumNitrateSoluble carbohydratesSymbiosis (lichen)

Abbreviations

CCCP

Carbonyl cyanide m-chlorophenylhydrazone

Chl

Chlorophyll

N

Nitrogen

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Lena Dahlman
    • 1
  • Jörgen Persson
    • 2
  • Kristin Palmqvist
    • 1
  • Torgny Näsholm
    • 2
  1. 1.Department of Ecology and Environmental ScienceUmeå UniversityUmeåSweden
  2. 2.Umeå Plant Science CenterDepartment of Forest Genetics and Plant PhysiologyUmeåSweden