Planta

, Volume 219, Issue 2, pp 233–242

Phloem-specific expression of the wound-inducible ribonuclease LE from tomato (Lycopersicon esculentum cv. Lukullus)

  • Margret Köck
  • Nadine Groß
  • Irene Stenzel
  • Gerd Hause
Original Article

DOI: 10.1007/s00425-004-1227-4

Cite this article as:
Köck, M., Groß, N., Stenzel, I. et al. Planta (2004) 219: 233. doi:10.1007/s00425-004-1227-4

Abstract

Ribonuclease LE (RNaseLE) from tomato (Lycopersicon esculentum Mill. cv. Lukullus) belongs to the widespread RNase T2 family of ribonucleases. With the exception of S-RNases of the solanaceous self-incompatibility system the functions of other members of the RNase T2 family are only barely understood. Using a 2.6-kbp putative promoter sequence of RNaseLE in front of the uidA reporter gene, expression of β-glucuronidase in developing phloem tissue and, especially, in the meristematic and elongation zones at root tips was detected. The tissue-specific expression accords with the range of cis-acting elements detected in the RNaseLE promoter. RNaseLE mRNA was localized in developing phloem cells but not in mature phloem tissue, suggesting association of RNaseLE expression with phloem development. Histochemical staining of β-glucuronidase activity as well as detailed inspection of RNaseLE at mRNA, protein and enzyme activity levels revealed that the wound-induced expression of RNaseLE was also restricted to vascular tissue. RNaseLE transcript accumulation detected by in situ hybridization occurred preferentially in phloem and cambial cells of stem sections upon wounding. The data provide evidence for a role of RNaseLE in a tissue-specific wound response and in wound healing of tomato.

Keywords

β-GlucuronidaseLycopersicon (wound response)PhloemPromoter studyRibonucleaseTissue specificity

Abbreviations

GUS

β-Glucuronidase (uidA gene)

RNaseLE

Tomato ribonuclease LE

Pi

Inorganic phosphate

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Margret Köck
    • 1
  • Nadine Groß
    • 1
  • Irene Stenzel
    • 1
    • 2
  • Gerd Hause
    • 1
  1. 1.BiocenterMartin Luther University of Halle-WittenbergHalleGermany
  2. 2.Department of Natural Product BiotechnologyInstitute of Plant BiochemistryHalleGermany