Planta

, Volume 218, Issue 5, pp 740–750

Up-regulation of Arabidopsis thaliana NHL10 in the hypersensitive response to Cucumber mosaic virus infection and in senescing leaves is controlled by signalling pathways that differ in salicylate involvement

  • Ming Shu Zheng
  • Hideki Takahashi
  • Atsushi Miyazaki
  • Hiroshi Hamamoto
  • Jyoti Shah
  • Isamu Yamaguchi
  • Tomonobu Kusano
Original Article

DOI: 10.1007/s00425-003-1169-2

Cite this article as:
Zheng, M.S., Takahashi, H., Miyazaki, A. et al. Planta (2004) 218: 740. doi:10.1007/s00425-003-1169-2

Abstract

The Arabidopsis thaliana (L.) Heyhn. genome contains 45 genes that show substantial sequence similarity to the tobacco (Nicotiana tabacum L.) HIN1 (harpin-induced) gene and the Arabidopsis NDR1 (non-race-specific disease resistance) gene. Of these, the nine (NHL1 to NHL3, NHL8 to NHL12 and NHL22) that bear the highest identity to HIN1 were selected and their expression in various situations was examined. We found that NHL10 behaves like the tobacco HIN1 gene in that its transcripts are abundant in senescing leaves and they specifically accumulate during the hypersensitive response (HR) caused by exposure to an avirulent Cucumber mosaic virus (CMV) strain. Spermine, a novel inducer of tobacco PR (pathogenesis-related) genes, was found to up-regulate expression of NHL3, NHL10 and NDR1. Green fluorescent protein-fusion experiments indicated that NHL2 and NHL10, and possibly NDR1 are localized in the chloroplasts. Studies using Arabidopsis mutants defective in salicylic acid (SA), ethylene and jasmonic acid signalling revealed that the senescence-associated expression of NHL10 is mediated by a pathway that involves SA but that NHL10 expression during CMV-induced HR and spermine treatment is totally independent of SA.

Keywords

Arabidopsis Cucumber mosaic virus Hypersensitive response HIN1/NDR1-like NHL10 Senescence 

Abbreviations

aa

Amino acid

CC

Coiled-coil

CMV

Cucumber mosaic virus

Col

Columbia

ET

Ethylene

GFP

Green fluorescent protein

HR

Hypersensitive response

HIN1

Harpin-induced gene

JA

Jasmonic acid

LRR

Leucine-rich repeat

NBS

Nucleotide-binding site

NDR1

Non-race-specific disease resistance

NHL

NDR1/HIN1-like

No

Nössen

PR

Pathogenesis-related

SA

Salicylic acid

Spm

Spermine

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Ming Shu Zheng
    • 1
  • Hideki Takahashi
    • 2
  • Atsushi Miyazaki
    • 1
  • Hiroshi Hamamoto
    • 3
  • Jyoti Shah
    • 4
  • Isamu Yamaguchi
    • 3
  • Tomonobu Kusano
    • 1
  1. 1.Graduate School of Life SciencesTohoku UniversityMiyagiJapan
  2. 2.Graduate School of Agricultural SciencesTohoku UniversityMiyagiJapan
  3. 3.RIKEN Plant Science CenterKanagawaJapan
  4. 4.Molecular Cellular and Developmental Biology Program, Division of BiologyKansas State UniversityManhattanUSA

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