Planta

, Volume 215, Issue 4, pp 523–532

SCB1, a BURP-domain protein gene, from developing soybean seed coats

  • Anthea K. Batchelor
  • Kim Boutilier
  • Shea S. Miller
  • Jiro Hattori
  • LuAnne Bowman
  • Ming Hu
  • Sylviane Lantin
  • Douglas A. Johnson
  • Brian L. Miki
Original Article

DOI: 10.1007/s00425-002-0798-1

Cite this article as:
Batchelor, A.K., Boutilier, K., Miller, S.S. et al. Planta (2002) 215: 523. doi:10.1007/s00425-002-0798-1

Abstract.

We describe a gene, SCB1 (Seed Coat BURP-domain protein 1), that is expressed specifically within the soybean (Glycine max [L.] Merrill) seed coat early in its development. Northern blot analysis and mRNA in situ hybridization revealed novel patterns of gene expression during seed development. SCB1 mRNA accumulated first within the developing thick-walled parenchyma cells of the inner integument and later in the thick- and thin-walled parenchyma cells of the outer integument. This occurred prior to the period of seed coat maturation and seed filling and before either of the layers started to degrade. SCB1 may therefore play a role in the differentiation of the seed coat parenchyma cells. In addition, the protein product appears to be located within cell walls. The SCB1 gene codes for a new member of a class of modular proteins that possess a carboxy-terminal BURP domain and a variety of different repeated sequences. The sequence of the genomic clone revealed the insertion of a Tgm transposable element in the upstream promoter region but it is not certain whether it contributes to the tissue-specific pattern of SCB1 expression.

BURP domain Gene expression (SCB1) Glycine (seed coat) Parenchyma cells Seed coat Tgm 

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Anthea K. Batchelor
    • 2
  • Kim Boutilier
    • 1
  • Shea S. Miller
    • 1
  • Jiro Hattori
    • 1
  • LuAnne Bowman
    • 1
  • Ming Hu
    • 1
  • Sylviane Lantin
    • 1
  • Douglas A. Johnson
    • 2
  • Brian L. Miki
    • 1
  1. 1.Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Ottawa, Ontario K1A 0C6 Canada
  2. 2.Biology Department, University of Ottawa, Ottawa, Ontario K1N 6N5 Canada

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