, Volume 215, Issue 4, pp 549–556

A conditionally fertile coi1 allele indicates cross-talk between plant hormone signalling pathways in Arabidopsis thaliana seeds and young seedlings

  • Christine Ellis
  • John G. Turner
Original Article

DOI: 10.1007/s00425-002-0787-4

Cite this article as:
Ellis, C. & Turner, J.G. Planta (2002) 215: 549. doi:10.1007/s00425-002-0787-4


Jasmonates (JAs) regulate Arabidopsis thaliana (L.) Heynh. wound and defense responses, pollen development, and stress-related growth inhibition. Significantly, each of these responses requires COI1, an F-box protein. We fused firefly luciferase as a reporter to the JA-responsive promoter for the vegetative storage protein gene (VSP) and used this to screen for mutants that failed to express luciferase in the presence of JA, isolating a mutant designated coi1-16. Comparisons with coi1-1 and jar1-1 plants indicated that coi1-16 was only slightly more sensitive to JA than coi1-1 plants. However, whilst coi1-16 plants failed to produce viable pollen at 22 °C, they were fertile at 16 °C. Therefore, unlike the other coi1 mutants, coi1-16 could be maintained as a pure line and did not require selection. We have used coi1-16 seeds to define novel interactions between JA and other hormone signalling pathways in seed germination and in the development of young seedlings.

Abscisic acid Arabidopsis (signaling pathways) COI1 Ethylene Germination Jasmonate

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Christine Ellis
    • 1
  • John G. Turner
    • 1
  1. 1.School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, UK