Pflügers Archiv

, Volume 442, Issue 3, pp 467–474

Phosphocreatine and ATP content in human single muscle fibres before and after maximum dynamic exercise

  • C. Karatzaferi
  • A. de Haan
  • R. Ferguson
  • W. van Mechelen
  • A. Sargeant
Original Article

DOI: 10.1007/s004240100552

Cite this article as:
Karatzaferi, C., de Haan, A., Ferguson, R. et al. Pflügers Arch - Eur J Physiol (2001) 442: 467. doi:10.1007/s004240100552

Abstract.

The recovery of high-energy phosphate levels in single human skeletal muscle fibres following short-term maximal (all-out) exercise was investigated. Three male volunteers exercised maximally for 25 s on an isokinetic cycling ergometer. Muscle biopsy samples from the vastus lateralis were collected at rest, immediately post-exercise and at 1.5 min of recovery. The subjects also performed a second exercise bout 1.5 min after the first, on a separate occasion. Single muscle fibres were dissected, characterized and assigned to one of four groups according to their myosin heavy chain (MyHC) isoform content; namely, type I, IIA, IIAx and IIXa (the latter two groups containing either less or more than 50% IIX MyHC). Fibres were analysed for adenosine 5′-triphosphate (ATP), inosine-5′-monophosphate (IMP), phosphocreatine (PCr) and creatine (Cr) levels. Type I fibres had a lower Cr content than type II fibres (P<0.01). Within type II fibres resting [PCr] increased with increasing MyHC IIX isoform content (r=0.59, P<0.01). Post-exercise [PCr] was very low in all fibre groups (P<0.01 versus rest) while great reductions in ATP were also observed (P<0.01 versus rest), especially in the type II fibre groups. [PCr] at 1.5 min of recovery was still lower compared to rest for all fibre groups (P<0.01) especially in the IIAx and IIXa fibres.

Fatigue High-energy phosphates Isokinetic cycling Recovery 

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • C. Karatzaferi
    • 1
  • A. de Haan
    • 2
  • R. Ferguson
    • 2
  • W. van Mechelen
    • 4
  • A. Sargeant
    • 2
  1. 1.Department of Biochemistry/Biophysics, Box 0948, San Francisco, CA 94143USA
  2. 2.Neuromuscular Biology Research Group, Manchester Metropolitan University, Alsager ST7 2HLUK
  3. 3.Institute for Fundamental and Clinical Human Movement Sciences, Vrije University, Van der Boechorststraat 9, Amsterdam 1081 BTThe Netherlands
  4. 4.EMGO Institute and Department of Social Medicine, Faculty of Medicine, Vrije University, Van der Boechorststraat 9, Amsterdam 1081 BTThe Netherlands
  5. 5.Present address: Sport Science Group, PESLS, The University of Edinburgh, Cramond Road North, Edinburgh EH4 6JD, e-mail: C.Karatzaferi@ed.ac.uk, Tel.: +44-131-3126001, Fax: +44-131-3126335UK