Pflügers Archiv

, Volume 436, Issue 1, pp 141–151

Quantitative analysis of dual whole-cell voltage-clamp determination of gap junctional conductance

  • H. V. M. Van Rijen
  • Ronald Wilders
  • Antoni C. G. Van Ginneken
  • Habo J. Jongsma
INSTRUMENTS AND TECHNIQUES

DOI: 10.1007/s004240050615

Cite this article as:
Van Rijen, H., Wilders, R., Van Ginneken, A. et al. Pflügers Arch (1998) 436: 141. doi:10.1007/s004240050615

Abstract

 The dual whole-cell voltage-clamp technique is used widely for determination of kinetics and conductance of gap junctions. The use of this technique may, however, occasion to considerable errors. We have analysed the errors in steady state junctional conductance measurements under different experimental conditions. The errors in measured junctional conductance induced by series resistance alone, and by series resistance in combination with membrane resistance, were quantified both theoretically and experimentally, on equivalent resistive circuits with known resistance values in a dual voltage-clamp setup. We present and analyse a method that accounts for series resistance and membrane resistance in the determination of true junctional conductance. This method requires that series resistance is determined during the experiment, and involves some calculations to determine membrane resistance. We demonstrate that correction for both membrane and series resistance reduces the error in measured junctional conductance to near zero, even when membrane resistances on both sides of the gap junction are as low as 20 MΩ and the (true) junctional conductance is as high as 100 nS.

Key words Series resistance Membrane resistance Mathematical modelling Equivalent resistive circuits Off-line correction methods 

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • H. V. M. Van Rijen
    • 1
  • Ronald Wilders
    • 1
  • Antoni C. G. Van Ginneken
    • 2
  • Habo J. Jongsma
    • 1
  1. 1.Department of Medical Physiology and Sports Medicine, Faculty of Medicine, Utrecht University, P.O. Box 80043, NL-3508 TA Utrecht, The NetherlandsNL
  2. 2.Academic Medical Center, University of Amsterdam, Department of Physiology, Meibergdreef 15, NL-1105 AZ Amsterdam, The NetherlandsNL