Pflügers Archiv

, Volume 433, Issue 6, pp 803–808

Hypoxia and cobalt stimulate vascular endothelial growth factor receptor gene expression in rats

  • P. Sandner
  • Konrad Wolf
  • Ulrike Bergmaier
  • Bernhard Gess
  • Armin Kurtz
ORIGINAL ARTICLE

DOI: 10.1007/s004240050348

Cite this article as:
Sandner, P., Wolf, K., Bergmaier, U. et al. Pflügers Arch (1997) 433: 803. doi:10.1007/s004240050348

Abstract

 This study aimed to examine the influence of acute tissue hypo-oxygenation on the expression of the vascular endothelial growth factor (VEGF) receptor genes. To this end male Sprague-Dawley rats were exposed to different hypoxic conditions such as 10% or 8% oxygen, 0.1% carbon monoxide and cobalt chloride (60 mg/kg) for 6 h and the abundance of flt-1, flt-4 and flk-1 mRNA in lungs and livers was determined by RNase protection assay. The relative proportions of flt-1, flt-4 and flk-1 were 10 : 2.5 : 1 and 10 : 10 : 2 in normoxic lungs and livers, respectively. It was found that 8% but not 10% oxygen increased flt-1 mRNA two- to threefold in both organs, whilst flt-4 and flk-1 mRNA were not changed by acute inspiratory hypoxia. Carbon monoxide inhalation also increased flt-1 mRNA but not flt-4 or flk-1 mRNA in both organs. Subcutaneous cobalt administration increased flt-1 mRNA in the livers only, whilst flt-4 and flk-1 mRNA remained unchanged. These findings show that acute tissue hypo-oxygenation is a rather selective stimulus for flt-1 gene expression. The efficiency of the different manoeuvres applied to stimulate flt-1 gene expression is rather similar to the stimulation of erythropoietin gene expression. It is not unreasonable to assume, therefore, that the oxygen-dependent regulation of both genes at the cellular level has significant similarities.

Key words Vascular endothelial growth factorTyrosine kinaseflt-1flt-4flk-1Erythropoietin

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • P. Sandner
    • 1
  • Konrad Wolf
    • 1
  • Ulrike Bergmaier
    • 1
  • Bernhard Gess
    • 1
  • Armin Kurtz
    • 1
  1. 1.Institut für Physiologie I, Universität Regensburg, D-93040 Regensburg, GermanyDE