Phosphoinositide substrates of myotubularin affect voltage-activated Ca2+ release in skeletal muscle

  • Estela González Rodríguez
  • Romain Lefebvre
  • Dóra Bodnár
  • Claude Legrand
  • Peter Szentesi
  • János Vincze
  • Karine Poulard
  • Justine Bertrand-Michel
  • Laszlo Csernoch
  • Anna Buj-Bello
  • Vincent Jacquemond
Muscle physiology

DOI: 10.1007/s00424-013-1346-5

Cite this article as:
González Rodríguez, E., Lefebvre, R., Bodnár, D. et al. Pflugers Arch - Eur J Physiol (2014) 466: 973. doi:10.1007/s00424-013-1346-5

Abstract

Skeletal muscle excitation–contraction (E–C) coupling is altered in several models of phosphatidylinositol phosphate (PtdInsP) phosphatase deficiency and ryanodine receptor activity measured in vitro was reported to be affected by certain PtdInsPs, thus prompting investigation of the physiological role of PtdInsPs in E–C coupling. We measured intracellular Ca2+ transients in voltage-clamped mouse muscle fibres microinjected with a solution containing a PtdInsP substrate (PtdIns(3,5)P2 or PtdIns(3)P) or product (PtdIns(5)P or PtdIns) of the myotubularin phosphatase MTM1. No significant change was observed in the presence of either PtdIns(5)P or PtdIns but peak SR Ca2+ release was depressed by ~30% and 50% in fibres injected with PtdIns(3,5)P2 and PtdIns(3)P, respectively, with no concurrent alteration in the membrane current signals associated with the DHPR function as well as in the voltage dependence of Ca2+ release inactivation. In permeabilized muscle fibres, the frequency of spontaneous Ca2+ release events was depressed in the presence of the three tested phosphorylated forms of PtdInsP with PtdIns(3,5)P2 being the most effective, leading to an almost complete disappearance of Ca2+ release events. Results support the possibility that pathological accumulation of MTM1 substrates may acutely depress ryanodine receptor-mediated Ca2+ release. Overexpression of a mCherry-tagged form of MTM1 in muscle fibres revealed a striated pattern consistent with the triadic area. Ca2+ release remained although unaffected by MTM1 overexpression and was also unaffected by the PtdIns-3-kinase inhibitor LY2940002, suggesting that the 3-phosphorylated PtdIns lipids active on voltage-activated Ca2+ release are inherently maintained at a low level, inefficient on Ca2+ release in normal conditions.

Keywords

Calcium homeostasis Excitation–contraction coupling Ryanodine receptor Sarcoplasmic reticulum Ca2+ release Phosphatidylinositol phosphate 

Supplementary material

424_2013_1346_MOESM1_ESM.pdf (510 kb)
ESM 1(PDF 544 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Estela González Rodríguez
    • 1
  • Romain Lefebvre
    • 2
  • Dóra Bodnár
    • 3
  • Claude Legrand
    • 2
  • Peter Szentesi
    • 3
  • János Vincze
    • 3
  • Karine Poulard
    • 4
  • Justine Bertrand-Michel
    • 5
  • Laszlo Csernoch
    • 3
  • Anna Buj-Bello
    • 4
  • Vincent Jacquemond
    • 2
  1. 1.Departamento de Fisiología, Genética y MicrobiologíaUniversidad de AlicanteAlicanteSpain
  2. 2.Centre de Génétique et de Physiologie Moléculaire et CellulaireUniversité Lyon 1, UMR CNRS 5534VilleurbanneFrance
  3. 3.Department of PhysiologyUniversity of DebrecenDebrecenHungary
  4. 4.Department of Research and DevelopmentGénéthon, INSERMEvryFrance
  5. 5.Lipidomic Core Facility, Metatoul Platform, INSERM U1048Université de Toulouse, Université Paul SabatierToulouseFrance