Invited Review

Pflügers Archiv - European Journal of Physiology

, Volume 465, Issue 3, pp 409-417

Manipulating signaling at will: chemically-inducible dimerization (CID) techniques resolve problems in cell biology

  • Robert DeRoseAffiliated withDepartment of Cell Biology, Center for Cell Dynamics, School of Medicine, Johns Hopkins University
  • , Takafumi MiyamotoAffiliated withDepartment of Cell Biology, Center for Cell Dynamics, School of Medicine, Johns Hopkins University
  • , Takanari InoueAffiliated withDepartment of Cell Biology, Center for Cell Dynamics, School of Medicine, Johns Hopkins University Email author 

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Abstract

Chemically-inducible dimerization (CID) is a powerful tool that has proved useful in solving numerous problems in cell biology and related fields. In this review, we focus on case studies where CID was able to provide insight into otherwise refractory problems. Of particular interest are the cases of lipid second messengers and small GTPases, where the “signaling paradox” (how a small pool of signaling molecules can generate a large range of responses) can be at least partly explained through results gleaned from CID experiments. We also discuss several recent technical advances that provide improved specificity in CID action, novel CID substrates that allow simultaneous orthogonal manipulation of multiple systems in one cell, and several applications that move beyond the traditional CID technique of moving a protein of interest to a specific spatiotemporal location.

Keywords

Chemically-inducible dimerization Rapamycin Signaling paradox