Pflügers Archiv - European Journal of Physiology

, Volume 465, Issue 2, pp 197–208

Postnatal exposure to voluntary exercise but not the antioxidant catechin protects the vasculature after a switch to an atherogenic environment in middle-age mice

  • Francois Leblond
  • Albert Nguyen
  • Virginie Bolduc
  • Jean Lambert
  • Carol Yu
  • Natacha Duquette
  • Eric Thorin
Integrative Physiology

DOI: 10.1007/s00424-012-1206-8

Cite this article as:
Leblond, F., Nguyen, A., Bolduc, V. et al. Pflugers Arch - Eur J Physiol (2013) 465: 197. doi:10.1007/s00424-012-1206-8

Abstract

We aimed to evaluate the lasting functional imprinting of exercise (EX) and catechin (CAT) on the vascular function of middle-age mice switched to a proatherogenic environment. C57BL/6J mice (n = 10–15 in each group) fed a regular diet (RD) were exposed from the age of 1 to 9 months either to EX (voluntary running; 2.7 ± 0.2 km/day), to the polyphenol CAT (30 mg/kg/day in drinking water), or to physical inactivity (PI). At 9 months of age, EX and CAT were stopped and mice either remained on the RD or were fed a Western diet (WD) for an additional 3 months. At 12 months of age, mice from all groups fed a WD had similar body mass, systolic blood pressure, and plasma total cholesterol, glucose, insulin, and isoprostane. Compared to the RD, the WD induced an indomethacin-sensitive aortic endothelium-dependent and independent dysfunction in PI mice (p < 0.05) that was prevented by both EX and CAT; this benefit was associated with a higher (p < 0.05) non-nitric oxide/non-prostacyclin endothelium-dependent relaxation. While EX, but not PI or CAT, prevented vascular dysfunction induced by the WD in cerebral arteries, it had no effect in femoral arteries. The profiles of activity of antioxidant enzymes and of proinflammatory gene expression in the aorta suggest a better adaptation of EX > CAT > PI mice to stress. In conclusion, our data suggest that a postnatal exposure to EX, but not to CAT, imprints an adaptive defense capacity in the vasculature against a deleterious change in lifestyle.

Keywords

Physical inactivity Western diet Endothelium Antioxidant enzymes Vascular smooth muscle cells 

Supplementary material

424_2012_1206_MOESM1_ESM.docx (16.2 mb)
ESM (DOCX 16589 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Francois Leblond
    • 1
    • 4
  • Albert Nguyen
    • 1
    • 4
  • Virginie Bolduc
    • 1
    • 4
  • Jean Lambert
    • 2
  • Carol Yu
    • 1
    • 4
  • Natacha Duquette
    • 4
  • Eric Thorin
    • 1
    • 3
    • 4
  1. 1.Department of Pharmacology, Faculty of MedicineUniversité de MontréalMontrealCanada
  2. 2.Department of Social and Preventive Medicine, Faculty of MedicineUniversité de MontréalMontrealCanada
  3. 3.Department of Surgery, Faculty of MedicineUniversité de MontréalMontrealCanada
  4. 4.Research CentreMontreal Heart InstituteMontrealCanada

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