Ion channels, receptors and transporters

Pflügers Archiv - European Journal of Physiology

, Volume 464, Issue 6, pp 613-621

First online:

Advanced glycation end products promote proliferation of cardiac fibroblasts by upregulation of KCa3.1 channels

  • Li-Mei ZhaoAffiliated withDepartment of Physiology and Pathophysiology, School of Medicine, Xi’an Jiaotong University
  • , Wei ZhangAffiliated withDepartment of Physiology and Pathophysiology, School of Medicine, Xi’an Jiaotong University
  • , Li-Ping WangAffiliated withDepartment of Physiology and Pathophysiology, School of Medicine, Xi’an Jiaotong University
  • , Gui-Rong LiAffiliated withDepartment of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong KongDepartment of Physiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong
  • , Xiu-Ling DengAffiliated withDepartment of Physiology and Pathophysiology, School of Medicine, Xi’an Jiaotong UniversityCardiovascular Research Center, School of Medicine, Xi’an Jiaotong University Email author 

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Abstract

The present study was designed to investigate whether advanced glycation end products (AGEs) would regulate KCa3.1 channels in cardiac fibroblasts and participate in cell proliferation. Cultured adult rat cardiac fibroblasts were employed to investigate the regulation of KCa3.1 channels by advanced glycation end products–bovine serum albumin (AGE–BSA) and the role of KCa3.1 channels in cell proliferation using approaches of molecular biology. KCa3.1 channel mRNA and protein levels were greatly enhanced in cardiac fibroblasts treated with 200 μg/ml AGE–BSA, and the effects were countered by anti-RAGE antibody or the ERK1/2 inhibitor PD98059, the p38-MAPK inhibitor SB203580, and the PI3K/Akt inhibitor LY294002. In addition, AGE–BSA stimulated cell proliferation and collagen production in cultured cardiac fibroblasts, and the effects were reversed by KCa3.1 blocker TRAM-34, anti-RAGE antibody, or signal inhibitors PD98059, SB203580, and LY294002. These results demonstrate for the first time that AGEs increase the expression of KCa3.1 channels in a RAGE-dependent manner and promote cardiac fibroblast proliferation and collagen production, which is mediated by phosphorylation of ERK1/2, p38-MAPK, and PI3K/Akt signals.

Keywords

Intermediate-conductance Ca2+-activated K+ channel Advanced glycation end products Cardiac fibroblasts Proliferation