Pflügers Archiv - European Journal of Physiology

, 458:61

Thick ascending limb: the Na+:K+:2Cl co-transporter, NKCC2, and the calcium-sensing receptor, CaSR

Ion Channels, Receptors and Transporters

DOI: 10.1007/s00424-008-0607-1

Cite this article as:
Gamba, G. & Friedman, P.A. Pflugers Arch - Eur J Physiol (2009) 458: 61. doi:10.1007/s00424-008-0607-1


The thick ascending limb of Henle’s loop is a nephron segment that is vital to the formation of dilute and concentrated urine. This ability is accomplished by a consortium of functionally coupled proteins consisting of the apical Na+:K+:2Cl co-transporter, the K+ channel, and basolateral Cl channel that mediate electroneutral salt absorption. In thick ascending limbs, salt absorption is importantly regulated by the calcium-sensing receptor. Genetic or pharmacological disruption impairing the function of any of these proteins results in Bartter syndrome. The thick ascending limb is also an important site of Ca2+ and Mg2+ absorption. Calcium-sensing receptor activation inhibits cellular Ca2+ absorption induced by parathyroid hormone, as well as passive paracellular Ca2+ transport. The present review discusses these functions and their genetic and molecular regulation.


DiureticsHypertensionSalt transportRegulationPhosphorylationIsoforms

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  1. 1.Molecular Physiology UnitInstituto Nacional de Ciencias Médicas y Nutrición Salvador ZubiránMexico CityMexico
  2. 2.Instituto de Investigaciones BiomédicasUniversidad Nacional Autónoma de MéxicoMexico CityMexico
  3. 3.Department of Pharmacology and Chemical BiologyUniversity of Pittsburgh School of MedicinePittsburghUSA