Green tea seed oil reduces weight gain in C57BL/6J mice and influences adipocyte differentiation by suppressing peroxisome proliferator-activated receptor-γ

  • Na-Hyung Kim
  • Sun-Kyung Choi
  • Su-Jin Kim
  • Phil-Dong Moon
  • Hun-Sun Lim
  • In-Young Choi
  • Ho-Jeong Na
  • Hyo-Jin An
  • Noh-Yil Myung
  • Hyun-Ja Jeong
  • Jae-Young Um
  • Seung-Heon Hong
  • Hyung-Min Kim
Cardiovascular Physiology

DOI: 10.1007/s00424-008-0537-y

Cite this article as:
Kim, NH., Choi, SK., Kim, SJ. et al. Pflugers Arch - Eur J Physiol (2008) 457: 293. doi:10.1007/s00424-008-0537-y

Abstract

Given that tea contains a number of chemical constituents possessing medicinal and pharmacological properties, green tea seed is also believed to contain many biologically active compounds such as saponin, flavonoids, vitamins, and oil materials. However, little is known about the physiologic functions of green tea seed oil. The aim of this study is to investigate the anti-obesity effects of green tea seed oil in C57BL/6J mice and in preadipocyte 3T3L-1 cell lines. In vivo, three groups of mice were fed with a standard diet, a high-fat diet containing 30% shortening, or 30% of green tea seed oil based on a standard diet for 85 days. The levels of total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, triglyceride, glucose, and alanine aminotransferase in blood were analyzed at the end of the study. The mice given green tea seed oil gained less weight compared to mice given the shortening diet (p < 0.01). The plasma level of total cholesterol was decreased by a significant level of 32.4% in mice given the green tea seed oil compared to the mice given the shortening diet (p < 0.01). In addition, 3T3-L1 cells were treated for 2 days to evaluate effects of green tea seed oil on adipocyte differentiation. Green tea seed oil inhibited expression of peroxisome proliferator-activated receptor-γ2 and CCAAT/enhancer binding protein-α in adipocytes and adipose tissue from the experimental animals. These results indicate that the anti-obesity effects of green tea seed oil might be, in part, through suppression of transcription factors related to adipocyte differentiation.

Keywords

Green tea seed oil Anti-obesity Peroxisome proliferator-activated receptor-γ2 CCAAT/enhancer binding protein-α 

Abbreviations

ALT

alanine aminotransferase

BCA

bicinchoninic acid

C/EBPα

CCAAT/enhancer binding protein-α

DMEM

Dulbecco’s Modified Eagle’s medium

DMSO

dimethyl sulfoxide

EGCG

(−)-epigallocatechin gallate

FBS

fetal bovine serum

HDL

high-density lipoprotein

LDL

low-density lipoprotein

MTT

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

PBS

phosphate-buffered saline

PPAR

peroxisome proliferator-activated receptor

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Na-Hyung Kim
    • 1
  • Sun-Kyung Choi
    • 2
  • Su-Jin Kim
    • 6
  • Phil-Dong Moon
    • 1
  • Hun-Sun Lim
    • 3
  • In-Young Choi
    • 6
  • Ho-Jeong Na
    • 2
  • Hyo-Jin An
    • 1
  • Noh-Yil Myung
    • 4
  • Hyun-Ja Jeong
    • 5
  • Jae-Young Um
    • 1
  • Seung-Heon Hong
    • 6
  • Hyung-Min Kim
    • 1
  1. 1.Department of Pharmacology, College of Oriental Medicine, Institute of Oriental MedicineKyung Hee UniversitySeoulRepublic of Korea
  2. 2.Department of Oriental Pharmacy, College of PharmacyWonkwang UniversityIksanRepublic of Korea
  3. 3.Korea Medi Co. LtdSeoulRepublic of Korea
  4. 4.Acupuncture and Meridian Science Research CenterKyung Hee UniversitySeoulRepublic of Korea
  5. 5.Biochip Research CenterHoseo UniversityAsanRepublic of Korea
  6. 6.Vestibulocochlear Research Center, College of PharmacyWonkwang UniversityIksanRepublic of Korea

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