Pflügers Archiv - European Journal of Physiology

, Volume 454, Issue 5, pp 759–776

Signal transduction and information processing in mammalian taste buds

Invited Review

DOI: 10.1007/s00424-007-0247-x

Cite this article as:
Roper, S.D. Pflugers Arch - Eur J Physiol (2007) 454: 759. doi:10.1007/s00424-007-0247-x

Abstract

The molecular machinery for chemosensory transduction in taste buds has received considerable attention within the last decade. Consequently, we now know a great deal about sweet, bitter, and umami taste mechanisms and are gaining ground rapidly on salty and sour transduction. Sweet, bitter, and umami tastes are transduced by G-protein-coupled receptors. Salty taste may be transduced by epithelial Na channels similar to those found in renal tissues. Sour transduction appears to be initiated by intracellular acidification acting on acid-sensitive membrane proteins. Once a taste signal is generated in a taste cell, the subsequent steps involve secretion of neurotransmitters, including ATP and serotonin. It is now recognized that the cells responding to sweet, bitter, and umami taste stimuli do not possess synapses and instead secrete the neurotransmitter ATP via a novel mechanism not involving conventional vesicular exocytosis. ATP is believed to excite primary sensory afferent fibers that convey gustatory signals to the brain. In contrast, taste cells that do have synapses release serotonin in response to gustatory stimulation. The postsynaptic targets of serotonin have not yet been identified. Finally, ATP secreted from receptor cells also acts on neighboring taste cells to stimulate their release of serotonin. This suggests that there is important information processing and signal coding taking place in the mammalian taste bud after gustatory stimulation.

Keywords

Taste budTransductionATPSerotoninNeurotransmittersSweetBitterUmamiSaltySour

Introduction

This review discusses two of the more intriguing aspects of sensory mechanisms in taste, (a) signal transduction, including a brief description of the ligands, their receptors, and the early events in taste transduction, and (b) signal processing within the taste bud, including an overview of how gustatory information is encoded by the taste bud. I refer the reader to a number of excellent recent reviews that also cover these topics and features of central processing in gustation [14].

Signal transduction is discussed first. This topic includes interactions of ligands with ion channels and G protein-coupled taste receptors (GPCRs) and, for the latter, downstream intracellular second-messenger cascades. The current consensus is that there are five basic tastes—sour, salty, sweet, bitter, and umami. For sour and salty tastes, the initial transduction events are only incompletely understood, although they are the focus of intense investigation. For sweet, bitter, and umami tastes, considerable information has accumulated in the past few years. Other qualities such as fatty may also be basic tastes with their own unique signal-transduction pathways [5, 6], but this remains to be widely accepted.

After a discussion of transduction, I will analyze signal processing. The generation of intracellular signals within taste receptor cells leads to an important flow of information within taste buds. This information processing involves cell–cell communication and excitatory synaptic activation of primary sensory afferents, the latter of which ultimately carry gustatory signals to the hindbrain for central nervous system (CNS) processing.

Transduction of sour taste

Acidic taste stimuli

Sourness is mostly aversive unless combined with other tastes such as sweet. It is commonly accepted that sour taste is elicited by acids. Sour taste may function to protect against consuming excessive dietary acid and disturbing the body’s vital acid–base balance. It has also been hypothesized that sour taste helps to avoid spoiled foods or unripened fruit. All these explanations are speculation, however. One of the earliest examples of the culinary use of sour tastants, apart from citrus fruits, is vinegar (or vin aigre, sour wine). Folklore suggests that vinegar was first produced in Babylonia 5000 bce as a result of wine fermentation. Vinegar is principally a solution of acetic acid (∼700 mM to 1 M) having a pH of 2.3 to 2.6 (white vinegar). For comparison, the human detection threshold for acetic acid is ∼0.1 mM [7], a solution having pH∼3.9. Detection threshold for a common mineral acid, HCl, is 6–10 mM [8], ∼pH 2.

Is sourness evoked by the protons in an acid solution? Defining what is the precise stimulus in sour taste bears upon how sour taste is transduced by receptor cells, which is discussed below. Shallenberger [9] coined the term “common acidophore” for the functional group of sour-tasting compounds. He considered the common acidophore was the simple proton (or more precisely, the hydronium ion, H3O+, because a proton in aqueous solutions is bound to one or more water molecules). Nonetheless, he recognized that, in the case of organic acids, the undissociated or partially dissociated acid molecules also contribute to sour taste. Indeed, it has long been recognized that sourness is not correlated simply with the concentration of protons in the stimulus solution (i.e., the pH) [10]. Enigmatically, the sour intensity of dilute solutions of different organic and inorganic acids is only loosely correlated with their pH: acetic acid at pH 3.9 evokes sour taste in humans, but HCl at this pH is relatively free of taste. Recordings from the chorda tympani nerve in rats show that stimulating the tongue with a solution of acetic acid at pH 5.5 ([H+] ∼3 μM) generates a response that is matched by an HCl solution having a pH = 3 (i.e., [H+] = 1 mM), a 300-fold greater concentration of protons than the effective acetic acid stimulus [11].

A number of studies in human psychophysics as well as studies on animal gustatory nerves and taste cells have attempted to determine precisely which component(s) of acids, especially organic acids, is (are) the active stimulus. Johanningsmeier et al. [12] conducted a metastudy of published data on human psychophysical rankings of the sourness of dilute solutions of citric acid, along with an analysis of malic, lactic, and acetic acids. They concluded that the best fit for concentration–response relations was obtained when sour intensity was plotted as a function of the sum total concentration of all the protonated molecular species plus the concentration of [H+]. Plotting sour intensity versus pH alone (i.e., versus [H+]) produced a weaker correlation. In short, sourness was not a function of the concentration of protons in solution.

Figure 1 illustrates the calculations discussed in Johanningsmeier et al. [12] and Table 1 summarizes the sour stimuli commonly used in gustatory research.
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Fig. 1

Calculations discussed in Johanningsmeier et al. [12]

Sour transduction mechanisms

Implicit in the above discussion is that the ligand for sourness is the proton and/or protonated species in the extracellular solution, i.e., in the taste stimulus. At first glance, this seems obvious. Yet, Lyall et al. [13] made the important observation that the proximate stimulus in sour taste appears to be the intracellular proton concentration, i.e., pHi. Our laboratory has verified and extended this observation. Further, we showed that although applying an organic acid (citric) to taste buds decreased pHi broadly in all taste bud cells (and for that matter, the surrounding non-sensory epithelial cells), only a small subset of taste bud cells specifically responded to this ΔpHi with a sour transduction signal, a Ca2+ transient [14]. In the same work, we also showed [14] that the Ca2+ transient elicited by ΔpHi in the subset of sour-sensitive cells was generated by Ca2+ influx through voltage-gated calcium channels consequent to membrane depolarization. More recent findings, discussed later, indicate that these cells represent a specific type of taste cell (type III, presynaptic cell).

These observations fit well with the analysis of the effective stimulus for sour taste, discussed above. The protonated molecular forms of organic acids, especially the neutral, fully-protonated species such as HAcetate or H3Citrate readily permeate the cell membrane, enter the cytosol, and dissociate to release proton(s) inside the taste cell (Fig. 2). If present in high enough concentration (i.e., low pH), extracellular protons will also cross the cell membrane, presumably through ion channels and ion exchangers such as the sodium–hydrogen exchanger (found in taste cells [15]), and acidify the cytoplasm, thereby explaining the sour taste of relatively concentrated solutions of HCl and other mineral acids.
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Fig. 2

Schematic drawing showing a taste cell stimulated with acetic acid. Acetic acid in solution dissociates into a mixture of protonated acetic acid (HAcetate), acetate, and protons (H+), the latter of which are bound to one or more water molecules (H3O+). HAcetate, being uncharged, penetrates the lipid plasma membrane and enters the cytosol. Inside the cell, HAcetate dissociates and releases H+ (which binds to H2O, H3O+) and acetate, acidifying the cytosol (lowering pHi)

Parenthetically, other routes of entry for extracellular protons might also exist, such as amiloride-sensitive epithelial sodium channels (ENaC), which were shown to exhibit proton permeation in hamster taste cells [16]. Supporting this conclusion are behavioral studies on hamsters showing that amiloride reduces the aversive taste of acetic acid solutions [17]. However, blocking ENaC channels in mouse taste buds did not affect acid-stimulated taste responses [14], suggesting that it might not be possible to generalize the findings from the hamster model to mammalian sour-taste mechanisms as a whole. The role of ENaC in sour taste remains debatable.

In a sense, the notion that intracellular acidification is the proximate stimulus for sour taste does indeed implicate the proton as the “acidophore”, borrowing Shallenberger’s terminology. However, it is the intracellular, not the extracellular proton that is the active species, and this has implications for interpreting proposed acid-transduction mechanisms. The intracellular proton likely interacts with and binds to a cytoplasmic domain of membrane proteins.

Regrettably, however, the search to date for sour-taste transducers has focused attention mainly on mechanisms involving extracellular gating of one or another postulated acid-sensing channels or receptors. For instance, the family of acid-sensing ion channels (ASICs) is well established as comprising cation channels that are activated by extracellular protons in many tissues. Ugawa et al. [18] championed ASIC2a (formerly, MDEG1) as a candidate for sour taste based on his studies on rat taste cells and the sensitivity to acid solutions (varying extracellular pH) of ASIC2a expressed in oocytes. Lin and Kinnamon [19] reported that acid-stimulated ion currents in rat taste cells had many properties that could be ascribed to ASIC channels. However, Richter et al. [20] showed that ASIC2a channels are not even expressed in mouse taste buds, despite this species’ well-established ability to sense sour taste stimuli. Thus, ASIC2a channels are unlikely to represent a fundamental sour-taste mechanism in mammals.

Stevens et al. [21] proposed HCN1 and HCN4 channels for sour-taste transducers based on the expression of these channels in rat taste buds and the finding that extracellular acid stimulation modulated the hyperpolarization gated ion currents in rat taste cells. However, Richter et al. [14] found that blocking cyclic nucleotide-gated ion (HCN) channels with Cs+ had no effect on acid-evoked responses in mouse taste cells, challenging the generality of HCN channels as a basic mechanism in mammalian sour taste.

ENaC channels as mediators of sour taste have been mentioned above, but the absence of amiloride-sensitive acid taste responses is a serious challenge to this candidate for a sour-taste transducer. A possible caveat is that the channel inhibition by amiloride and other ENaC blockers might, itself, be acid-sensitive, such that the pharmacological agents are not active in low-pH solutions [22].

Another quite different possibility was recently put forward by Huque et al. [23], who speculated that GPR4, a proton-sensitive GPCR [24] that they found to be expressed in human fungiform papillae, was a sour receptor. These findings are intriguing, but a full report of the results remains to be published.

A recent addition to the gallery of candidate sour transducers is the transient receptor potential (TRP) family channel, PKD2L1, and its associated partner, PKD1L3. Lopez Jimenez et al. [25] first reported the expression of these channels in a subset of mouse taste cells. Later, Ishimaru et al. [26] confirmed and extended those findings by expressing PKD1L3 and PKD2L1 in HEK293 cells and showing that the transfected cells generated an inward current upon acid stimulation. Finally, Huang et al. [27] engineered mutant mice lacking the taste cells that express PKD2L1 and showed that these animals did not respond to sour-taste stimuli. A caveat for that finding is that the absence of an entire subpopulation of taste cells does not necessarily speak to the role of any given protein expressed by the missing cells. Nonetheless, collectively, the findings strongly implicate PKD2L1 and PKD1L3 as members of an acid-transducing matrix of channels (see next). Details about whether and to what extent PKD2L1 and PKD1L3 are modulated by intracellular pH remain to be established.

Lastly, Richter et al. [28] studied acid responses in mouse taste cells and concluded that a possible explanation for acid-taste transduction was the intracellular acidification of a matrix of membrane proteins, including certain K2P channels that are expressed in taste cells. This appears to be logical, given the likely potent effects of pHi on a variety of channels and receptors. For instance, TASK-1, a K2P channel found in taste cells, is blocked by intracellular (and extracellular) acidification [29]. Blocking TASK-1 would be expected to depolarize the membrane, open voltage-gated calcium channels, and admit Ca2+ to stimulate transmitter release.

In sum, taste bud cells involved in sour transduction have been identified, and some of the molecular details are emerging. Much remains to be discovered about the actual transduction machinery for acid stimuli, however. Attention has been focused on a search for a transducer protein(s) activated by extracellular protons, whereas the actual target appears more likely to be an intracellular site.

Transduction of salty taste

NaCl, the prototypic salt stimulus

The principal stimulus for salty is a common ion, Na+. Table salt, NaCl, is the prototypic salty tastant. Na+ and Cl are essential nutrients, vital for maintaining blood volume, blood pressure, regulating body water, and in the case of Cl, maintaining acid/base homeostasis (i.e., Cl shift). The detection threshold for NaCl is 1 to 7 mM in rodents and humans alike [3032]. The human recognition threshold for NaCl is complicated by the variety of tastes that dilute NaCl elicits, such as sweet [33]. Other ions, including ammonium, lithium, and potassium, also stimulate salty taste but have pronounced other tastes in addition. It is claimed that the dipeptides ornithyltaurine and ornithyl-β-alanine evoke salty tastes and are effective salt substitutes [34, 35], but these findings have been disputed [36]. The issue is important because the chemical nature of the stimulus gives important information about the receptor mechanisms, which for salty, remain somewhat illusive.

Salty chemosensory transduction

An early attempt to explain salty taste attributed transduction to a protein or nucleic acid in the plasma membrane of taste cells that bound Na+ [37]. A seminal finding was published by Heck et al. [38] in 1984 when they reported that Na+ permeates cation channels in taste cells, specifically, amiloride-sensitive ENaCs (similar to those found in the kidney) on the anterior tongue. Passive Na+ influx through ion channels, driven by the presence of Na salts in the stimulus (that is, a Na+ concentration gradient favoring Na+ influx), would depolarize and thereby excite taste receptor cells.

The search for ENaC subunits has shown that the α, β, and γ subunits of ENaC are indeed expressed in taste buds [39, 40]. Parenthetically, \( {\text{ENaC}}_{{\text{ $ \beta $ }}} \) and \( {\text{ENaC}}_{{\text{ $ \gamma $ }}} \) are less prominent in taste buds of the posterior tongue, possibly explaining the absence of amiloride-block for Na responses in taste buds from that region of the tongue. The presence of amiloride in NaCl solutions reduces the ability of rats and mice to distinguish KCl from NaCl, which they normally can readily do [41, 42], supporting a role for amiloride-sensitive channels, possibly ENaC, in Na+ taste. However, the effects of amiloride are less clear on the taste of NaCl in humans [43, 44], raising some doubt about the role of amiloride-sensitive ENaC channels as a universal explanation for salt taste. A powerful approach to study the involvement of these channels in salt taste would be to examine mutant mice lacking one or more of the ENaC subunits [45]. Unfortunately, these gene knockout mice die shortly after birth.

Alternatively, a vanilloid receptor, V1R-like, was postulated to transduce Na+ taste [46]. Subsequent behavioral studies using mutant mice lacking V1R (also known as TRPV1) did not reveal a lack of salt taste, however [47], challenging the hypothesis that this channel is involved in salt taste.

The consensus seems to be that there are both amiloride-sensitive and amiloride-insensitive pathways in salt taste in mammals. How these pathways map onto specific ENaC subunits, if at all, remains to be determined.

Anions of sodium salts contribute to salty taste, particularly regarding taste intensity. NaCl evokes a stronger saltiness than Na sulphate, for example. One explanation for this involves the ability of Na+ to permeate deep into the taste bud by penetrating through the tight junction spaces at the apical tips of cells [48]. The apical tips of taste bud cells, as in all epithelial boundaries, are sealed by a junctional complex including the zonula occudens. This is a specialized intercellular web that reduces access of ions and molecules in the external environment to the interstitial spaces of the taste bud. However, the apical cell–cell junctions are partially permeable to Na+ and Cl, representing a paracellular leakage for NaCl and allowing access to the interior of the taste bud. Large anions such as \( SO^{{2 - }}_{4} \), however, do not pass through the tight paracellular spaces and act as a brake, holding Na+ back. Movement of Na+ unaccompanied by anions creates a charge separation and retards further movement of cations. When Cl moves with Na+, there is no charge separation, and substantially more of the salt traverses the paracellular route. Penetration of NaCl into the interstitial spaces in the taste bud through apical paracellular pathways and the consequent increased intensity of salty-taste sensations suggest that chemosensory transduction mechanisms for Na+ may be present along the basolateral membranes of taste cells as well as on the apical tips of taste bud cells.

Transduction of sweet, bitter and umami tastes

Compounds that elicit sweet taste

The prototypic sweet stimulus is sucrose, a disaccharide. Humans can detect sucrose at a concentration of 6–7 mM [49] and recognize it as a sweet taste around 25 mM [50], although these values vary somewhat from study to study. A wide diversity of compounds elicit sweet taste (e.g., see [9]), including sugars, of course, such as monosaccharides (e.g., glucose, fructose, mannose), disaccharisdes (e.g., sucrose, lactose, maltose, trehalose), trisaccharides (e.g., raffinose), tetrasaccharides (stachyose), and, to some extent, even higher-level oligosaccharides (e.g., polycose, a partially hydrolyzed starch product). Some amino acids also elicit sweet taste, including glycine (from the greek, glykýs “sweet”), alanine, threonine, d-tryptophan, d-histidine (l-tryptophan and l-histidine are bitter), and others. Certain peptides and proteins are intensely sweet, most famously the methyl ester of the dipeptide l-aspartyl-l-phenylalanine (“aspartame”) and the proteins brazzein [51] [54 amino acids (a.a.), molecular weight (MW) 6,473], monellin (94 a.a., MW∼11,000) [52], and thaumatin (207 a.a., MW 22,000) [53]. Some alcohols, such as the aliphatic alcohols glycerol, sorbitol, and xylitol are sweet, too. In addition to these naturally-occurring sugars, alcohols, peptides, and proteins, there are a number of sweet synthetic compounds (ethylene glycol) and artificial sweeteners such as sucralose (“Splenda”, a derivative of sucrose with Cl substituted for three OH groups), saccharine, and a host of others. Even simple ions elicit sweet taste, including dilute solutions of NaCl (10–30 mM), LiCl, KCl, and dangerously, the salts of beryllium and lead. One readily observes that the list of sweet compounds is vast and taxes the imagination to derive any common feature. Perhaps the most exhaustive attempt to find a common theme among sweet tastants is the work initiated by Shallenberger and Acree [54] (also see http://www.nysaes.cornell.edu/fst/faculty/acree/ahb/acree70.html). These researchers derived a “glycophore” consisting of an electronegative atom (O or N) designated as “A” that forms a hydrogen bond “H” with another nearby (∼0.3 nm distant) electronegative atom, “B”, to form a leitmotif of AH–B found in all sweet compounds. This simple motif has been modified and expanded over the years in an effort to encompass all sweet compounds.

T1Rs are GPC receptors for sweet taste

The value of searching for a glycophore, among other things, was to help guide the isolation and purification of sweet-taste receptors. However, another approach, one utilizing molecular genetics, was eventually successful in identifying GPCRs that transduce sweet. Namely, in 1999, Hoon et al. [55] used differential screening of cDNA libraries constructed from single taste cells and from nontaste lingual tissue to identify two taste-specific GPCRs, TR1 and TR2 (later renamed T1R1 and T1R2). Hoon et al. speculated that, based on its pattern of expression on the tongue of these proteins, T1R1 (TR1) was a sweet receptor. Two years later, the human genome database that had just become available made it possible to search for genes related to T1R1 and T1R2. Several independent groups from USA and Japan scrutinized the human genome in a region comparable to a locus on chromosome 4 in mice, sac, that confers a taste preference for saccharin. This scan resulted in the identification of T1R3, a novel gene that was shown to be taste-specific and related to sweet-taste preference [5661].

T1R1, T1R2, and T1R3 are all members of the large class C GPCRs that are typified by an extensive extracellular N terminus. Max et al. [58] predicted that T1R3 forms a dimer in the plasma membrane, based on comparisons with other closely related class C GPCRs, and specifically with metabotropic glutamate receptor 1 (mGluR1). A few months later, T1R3 was indeed shown to form a dimeric functional receptor, principally with T1R2 [60, 62]. The dimer, T1R2+T1R3, when expressed in HEK293 cells, conferred responsivity to sugars, and thus, T1R2+T1R3 was firmly established as the first identified sweet-taste receptor (Table 2). Homodimers of T1R3 (T1R3/T1R3) may also function as a sweet receptor [63], as predicted earlier.
Table 2

Experiments designed to test the notion that T1R2+T1R3 dimer is a sweet receptor

Strategy

Findings

Heterologous expression

When mouse or human T1R2+T1R3 receptors are expressed in HEK 293 cells, they confer a sensitivity, absent in wild type cells, to a wide variety of sweet compounds, including sucrose, fructose, dulcin, saccharin, and others; and for human T1R2+T1R3, cyclamate, aspartate, monellin, and thaumatin [60, 62].

Molecular structure

Detailed investigations of T1R2 and T1R3 reveal specific sites for binding different sweet-tasting ligands [64, 66, 67, 69].

Genetic modification

Introducing T1R3 from a strain of mice in which sweet taste was normal (“taster” strain) into mice lacking a strong preference to sugars (“non-taster” strain) rescued and restored behavioral responses to saccharin and sucrose in the latter [60].

Mice genetically modified to express human T1R2 yield animals with sweet preferences found only in humans and not normally in mice [63].

Gene knock-out

Responses to sweet tastes are suppressed in mutant mice lacking T1R2 and T1R3 (T1R knockout mice). The findings and interpretations from different laboratories concerning these experiments and the primacy of T1R2+T1R3 in sweet taste are somewhat conflicting, however. The laboratories of N. Ryba and C. Zuker concluded that T1R2+T1R3 (and possibly the homodimer T1R3+T1R3) is the only receptor for sweet [63]. However, Damak et al. [98], using a different knockout strategy, reported and emphasized that T1R3 knockout mice had only diminished but otherwise measurable behavioral and nerve responses to sucrose, glucose, and other sugars. Recently, Delay et al. [90] reported that the detection threshold for sucrose was indistinguishable between T1R3 knockout and wild-type mice. Nonetheless, Damak et al. [98] and Zhao et al. [63] both report that responses to artificial sweeteners were absent in the T1R3 knockout mice.

In summary, the GPC dimeric taste receptor T1R2+T1R3 clearly is a central component of sweet taste, especially for artificial sweeteners. However, recent findings suggest there may be additional, yet undiscovered taste receptor(s) for sucrose and other sugars.

Where do ligands bind to T1R2 and T1R3?

Given the importance of the heterodimeric receptor T1R2+T1R3 for sweet taste, recent studies have focused on how sweet compounds activate this dimer. An important concept that has emerged is that there are multiple binding pockets with different selectivities for different ligands. This may help explain the ability of the receptor to respond to such a wide range of sweet-tasting compounds as described above. The laboratories of X. Li, R. Margolskee, S. Munger, and P. Temussi are actively investigating the ligand-binding domains of T1R2 and T1R3. Temussi [64] has taken the approach of using computer modeling of the sweet binding site of the T1R dimer. They have analyzed the large extracellular domain, which has been shown to form a hinged ligand-binding pocket. This pocket is the so-called Venus flytrap (VFT) motif, first identified in another class C GPCR, mGluR1. Morini et al. [65] determined that a small region in the VFT of T1R2 and T1R3 may bind sugars, sweet amino acids, and other sweet compounds, and produce a conformational change in the “trap”. It is uncertain whether the N termini of T1R2 and T1R3 interact allosterically in this binding or whether the two N termini function independently. Nie et al. [66] has used a novel technique to explore ligand binding of expressed N-terminal domains of mouse T1R2 and T1R3. They have measured subtle changes in the spectroscopic properties of the purified N termini when stimuli, including sweet-taste compounds, are added to the N termini. There are key binding sites for sugars in the large extracellular N termini of T1R2 and T1R3 alike.

Jiang et al. [67] took advantage of the observation that the protein brazzein elicits intense sweet taste in humans but not in mice to parse different regions of the T1R2+T1R3 dimer and locate a binding site for sweet proteins. They constructed chimeras of the mouse and human T1R1+T1R3 and located a cysteine-rich region in T1R3 near the base of the N terminus that appears to be a binding pocket for brazzein and other sweet-tasting proteins. Using chimeric constructs of human and rat T1R1+T1R3 [68], or human and mouse T1R2+T1R3 followed by site-directed mutagenesis [69], researchers have narrowed down the cyclamate-binding pocket to portions of transmembrane segments 3, 5, and 6 of T1R3 and extracellular loop 2, suggesting that this pocket is mainly burrowed into the membrane. This contrasts with the N-terminal, extracellular binding domains for sugars and sweet proteins. Moreover, the cyclamate-binding pocket also appears to be where certain antagonists such as lactisole interact with the sweet receptor [68, 69].

These multiple binding pockets for different sweet-tasting compounds are illustrated in Fig. 3.
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Fig. 3

Schematic drawing of the sweet GPCR dimer T1R2 + T1R3, showing the multiple ligand binding sites. T1R2 is shown on the left, T1R3 is on the right. Details of the interactions, if any, between the extensive N termini of T1R2 and T1R3 are not known. The N termini possess one or more binding pocket(s) for saccharin, sucrose, and other sugars. Another ligand-binding pocket, located in T1R3 near the first transmembrane region, exists for certain sweet-tasting proteins such as brazzein. A third ligand pocket, embedded in the transmembrane (TM) regions of T1R3, is comprised of portions of TM3, 5, and 6. This site binds the artificial sweetener, cyclamate, but also is the binding site for antagonists such as saccharin (which at high concentrations is a sweet receptor antagonist) and lactisole

Chemosensory transduction and the intracellular cascades initiated by the T1R2+T1R3 sweet receptor are discussed below, in combination with transduction for the other GPCR-mediated tastes, bitter and umami.

Compounds that elicit bitter taste

If the wide range of different sweet-tasting compounds is impressive, the diversity of bitter compounds is astounding. Meyerhof [2] has recently attempted to categorize bitter compounds and has provided the most useful characterization of the universe of these tastants. Among the more intensely bitter compounds are amides, such as denatonium benzoate, and alkaloids such as strychnine, caffeine, and quinine. However, certain amino acids, urea, fatty acids, phenols, amines, esters, and other compounds also elicit bitterness. Even salts of potassium, magnesium, and calcium taste bitter. Many plants contain bitter compounds, including nutritionally important foods such as Cruciferous vegetables (e.g., arugula, broccoli, cauliflower, cabbage, watercress, and bok choy).

Because bitterness is generally an aversive taste and because many naturally-occurring toxic compounds taste bitter, it is commonly believed that bitter taste evolved to prevent animals from consuming harmful foodstuffs. Consistent with this interpretation, bitter taste on the whole has a lower threshold for activation, presumably to prevent consuming even small quantities of toxins. For example, the human detection threshold for caffeine is 133 μM and for quinine is only 1.6 μM [70]. Naive, neonatal humans and non-human primates reject bitter tastes [71]. However, with experience, humans learn to accept and even seek out bitter-tasting foods and drinks. Indeed, an intensely bitter vegetable of the Cucurbitaceae family, Momordica charantia (bitter melon), even has its own society to promote its consumption, the National Bitter Melon Council (http://www.bittermelon.org/). Animals in the wild have been observed intentionally consuming bitter plants for purposes of self-medication [72]. In short, a wide diversity of compounds elicits bitterness, and conversely, bitterness elicits a variety of ingestive behaviors.

T2Rs are GPC receptors for bitter taste

GPCRs that transduce bitterness were first identified in 2000 by L. Buck’s, N. Ryba’s and C. Zuker’s groups [73, 74]. These researchers exploited the newly published human genome database to search for candidate GPCRs in regions associated with bitter taste. For instance, Matsunami et al. [74] searched a region of human chromosome 12 corresponding to the sucrose octaacetate (SOA) locus on mouse chromosome 6, a portion known to control bitter taste. Adler et al. [73] chose to search for novel GPCR genes on human chromosome 5 in an area related to the ability of humans to taste propylthiouracil, a bitter compound. Both groups uncovered a large family of novel, taste-specific GPCRs for sensing bitter. Presently, 36 bitter-taste receptor genes and seven pseudogenes have been identified in the mouse [75] and 25 genes and 11 pseudogenes in the human [76]. These bitter receptors have been named T2Rs (also termed Tas2Rs).

Heterologous expression of the newly cloned mouse and human T2Rs in HEK293 cells confers the ability to respond to denatonium and cycloheximide, two intensely bitter compounds [77], verifying their role as bitter receptors. The role of T2Rs in bitter taste was confirmed and extended by more recent studies in native tissues: mice, genetically engineered to express candidate human bitter receptors such as hTAS2R38 for taste stimuli to which rodents normally do not respond (in the case of hTAS2R38, phenylthiocarbamide, see Table 3), manifest a taste aversion to those compounds [78]. In addition, mice expressing a κ-opioid receptor under the control of a bitter receptor promoter show a taste aversion to spiradoline, an opioid agonist that is normally tasteless to mice [78]. These studies validate the role of T2Rs (Tas2Rs) in bitter taste.
Table 3

Human bitter taste receptors and their agonists

Receptor [citation]

Ligand

hTas2R16 [148]

Salicin (a bitter-tasting beta-glucopyranoside found in the bark of willows and related to aspirin, acetylsalicylic acid)

hTAS2R38 [149]

Phenylthiocarbamide (PTC), propylthiouracil (PROP)

hTAS2R43, hTAS2R44 [150]

High concentrations of saccharin and acesulfame K (artificial sweeteners that evoke a bitter aftertaste in humans)

hTas2R46 [81]

Absinthin, strychnine, and denatonium (the latter being one of the most intensely bitter compounds for humans and used, for example, as a deterrent for nail biting and thumb sucking)

A focused search for ligands that activate the 25 human T2Rs (or hTas2Rs as the human bitter receptors are termed) is presently underway with at least five bitter-taste receptors and their ligands identified to date (Table 3).

Initially, it was reported that each bitter-responsive taste receptor cell expresses nearly the entire family of 2–3 dozen T2Rs [73]. This conclusion was based upon in situ hybridization (ISH) analyses in mouse taste buds. Namely, ISH with a mixture of probes for several mT2Rs did not label a significantly greater population of taste cells than ISH carried out with a probe for only one mT2R. This and related findings led Adler et al. [73] to conclude that there is a labeled line for bitter taste: any and all bitter compounds would stimulate a homogeneous population of T2R-expressing taste cells having a functional label, “bitter”. These data conflicted with Ca2+ imaging studies showing that individual bitter-sensing cells responded to only one or a few bitter compounds and not broadly to all bitter tastants [79]. The issue appears to have been resolved recently by the careful ISH studies of Behrens et al. [80] using probes for hTAS2Rs. Bitter-sensing taste receptor cells do indeed express only subsets of TAS2Rs, not the entire family of receptors, at least in humans.

Where do ligands bind to T2Rs?

The structure of the bitter-taste GPCRs is strikingly different from that of the sweet-taste receptors. Namely, there is no evidence yet that the T2Rs (Tas2Rs) form dimers. Moreover, the extracellular N terminus of the bitter receptors is short and may not play a prominent role in ligand recognition. In this regard, the bitter-taste receptors are more similar to the class A (rhodopsin-like) GPCRs, although strictly speaking, they are not formally considered members of that category. Bitter-taste receptors form a class of their own.

Ongoing efforts are aimed at determining the agonist interaction sites for bitter receptors, similar to efforts discussed above for the sweet receptor. The extracellular loops and transmembrane domains that vary considerably from receptor to receptor within the family of bitter receptors are likely regions for these binding sites [81].

Compounds that elicit the taste, umami

The prototypic taste compound eliciting the savory or meaty taste termed “umami” (roughly translated from Japanese as “good taste”) is the sodium salt of glutamate, or monosodium glutamate (MSG). This compound was extracted from dried kelp, a flavor additive in Oriental cuisines, and shown to be responsible for umami taste in 1908 by Kikunae Ikeda (English translation, [82]). Other compounds also elicit umami taste, namely, certain inosine and guanosine derivatives (inosine 5′-monophosphate, IMP; guanosine, 5′-monophosphate GMP). When presented in combination with MSG, IMP or GMP accentuate the umami taste in an unexplained synergistic mechanism. Glutamate and the nucleotide monophosphates occur naturally in many foods, especially meats, cheeses, tomatoes, even mother’s milk, imparting umami taste. Additional compounds that also elicit umami taste include a select few amino acids other than glutamate (e.g., aspartate), certain di-, tri-, and higher-order peptides [8385], synthetic glutamate-like compounds (l-AP4), and some organic acids [86].

G protein-coupled receptors for umami

A number of receptors for umami taste have been proposed over the past decade, beginning with the surmise of Faurion [87] that the umami-taste receptor might resemble brain synaptic receptors where glutamate is a neurotransmitter. A major difference between synaptic glutamate receptors and glutamate-taste (umami) receptors is the operating range for the ligand concentration. For central synapses, EC50 for glutamate is ≤100 μM [88, 89]. In marked contrast, the threshold for MSG-taste detection in mice, rats, and humans is over tenfold higher, 1 to 2.5 mM [9092].

A candidate GPCR for umami taste was first identified by reverse transcriptase polymerase chain reaction (PCR) using primers for synaptic glutamate receptors, as Faurion had conjectured. Chaudhari et al. [93, 94] identified a truncated N-terminal version of mGluR4 in rat taste buds and showed that Chinese hamster ovary (CHO) cells expressing this receptor responded to l-glutamate in a concentration range appropriate for umami taste. Yang et al. [95] showed by ISH that mGluR4 was expressed in a subset of rat taste bud cells. Tests using genetic mutant mice lacking mGluR4 (“knockout mice”) indeed showed significant behavioral alterations to the taste of MSG and not to NaCl, sucrose, or saccharin [96]. Curiously, however, mGluR4 knockout mice preferred MSG to a greater extent than wild-type mice. However, these results were difficult to interpret because synaptic-mGluR4 and taste-mGluR4 alike were missing in the mutant strain. The absence of synaptic mGluR4 in the brain may well have influenced the perception of MSG and the taste behavioral assay.

Subsequently, in heterologous expression studies, investigators identified another candidate umami receptor, a dimer of T1R receptors, T1R1+T1R3 [62, 97]. HEK293 cells expressing this combination of T1Rs responded to glutamate and a number of other amino acids. Furthermore, adding low concentrations of IMP appeared to boost the responses to glutamate and other amino acids, mimicking the synergy reported for glutamate and nucleotide monophosphates in behavioral and nerve-recording studies. An important test for the role of T1R1+T1R3 in umami taste was conducted by Zhao et al. [63]. They reported that mutant mice lacking either of these receptors showed a total absence of nerve responses or behavioral responses to MSG and other amino acids. However, other laboratories that have tried to replicate those studies reported somewhat different findings. Namely, Damak et al. [98] recorded reduced but still appreciable nerve and behavioral responses to glutamate in T1R3-null mice. Maruyama et al. [99] extended these studies by recording responses from individual taste cells in lingual slice preparations from mutant and wild-type mice. They also showed that although umami responses were somewhat diminished in T1R3-null mice, nonetheless, many cells responded to umami-taste stimulation. Collectively, the findings indicate that the taste of umami is likely to be transduced by T1R1+T1R3 dimers as well as other receptors, possibly taste-mGluR4, or by the newly reported truncated mGluR1 [100], or by as yet undiscovered receptors. It is possible that mGluR-taste receptors might themselves oligomerize, conceivably even with T1Rs, but this has not been tested as of yet.

Table 4 summarizes the discovery of taste GPCRs.
Table 4

Brief history of the discovery of candidate taste GPCRs

Year

Taste receptor

Citations

Taste stimulus

Strategy

1999

TR1, TR2 renamed T1R1, T1R2 (Adler et al. [73])

Members of the large family of class C GPCRs, typified by lengthy N termini having a Venus flytrap motif

Hoon et al. [55]

Unknown in 1999

Large-scale sequencing of a subtracted cDNA library (nontaste–taste cell cDNA) derived from lingual tissue

2000

Taste-mGluR4

N terminal truncated version of the metabotropic glutamate receptor, mGluR4, also a class C GPCR

Chaudhari et al. [92]

Umami (monosodium glutamate)

Focussed search with primers based on synaptic glutamate receptors

2000

T2Rs (also known as Tas2Rs, Bufe et al. [148])

A large family of GPCRs (N ∼ 40 in mice) with short N termini. The T2Rs are most closely related to the class A (rhodopsin-like) GPCRs but are considered as a separate category.

Adler et al. [73]; Chandrashekar et al. [77]; Matsunami et al. [74]

Bitter

Data mining of newly available human genome for GPCRs in a region associated with the ability to taste PROP or SOA

2001

T1R3 (also known as Tas1r3)

A class C GPCR similar to T1R1 and T1R3

Bachmanov et al. [56], Kitagawa et al. [57], Max et al. [58], Montmayeur et al. [59], Nelson et al. [60], Sainz et al. [61]

Sweet

Data mining of newly available human genome for GPCRs in the human equivalent of the sac locus from mice

2001–2003

T1R2/T1R3 (and possibly T1R3/T1R3)

A heterodimer of T1R2+T1R3

Nelson et al. [60], Li et al. [62], Zhao et al. [63]

Sweet

Heterologous expression in HEK293 cells

2003

T1R1/T1R3 dimer

A heterodimer of T1R1+T1R3

Zhao et al. [63]

Umami (monosodium glutamate)

Heterologous expression in HEK293 cells

2005

Taste-mGluR1

N terminal truncated version of mGluR1, also a class C GPCR

San Gabriel [100]

Umami (monosodium glutamate)

Focussed search with primers based on synaptic glutamate receptors

Second-messenger cascades and taste GPCRs

Cyclic adenosine monophosphate as a second messenger in taste

Two major streams of intracellular molecular interactions appear to be triggered by taste stimulation of taste GPCRs (i.e., sweet, bitter, and umami). The earliest identified taste-transduction stream involved the second messenger cyclic adenosine monophosphate (cAMP). Decades ago, Striem et al. [101] reported that treating membranes derived from the anterior surface of rat tongues with sucrose stimulated adenylyl cyclase, implying the presence of \( {\text{G}}_{{{\text{ $ \alpha $ s}}}} \) proteins and cAMP pathways in taste. These studies have been replicated and extended, using intact taste tissue [102]. Further, cAMP depolarizes frog and mouse taste cells, presumably due to cAMP-dependent protein kinase-phosphorylating and closing potassium channels [103, 104]. Fast quench flow measurement of second messengers after taste stimulation in rat taste buds revealed that sucrose induces an initial increase in cyclic GMP earlier and more transiently than changes in cAMP [105]. The significance of an early transient cyclic nucleotide signal is unclear. Krizhanovsky et al. [105] speculated that changes in intracellular cAMP occur later during taste stimulation and might be involved in more long-term effects, such as adaptation.

In 1992, McLaughlin et al. [106] identified a taste-specific G protein, \( {\text{G}}_{{\text{ $ \alpha $ }}} \) gustducin, that resembles transducin. By analogy with transducin, taste receptor activation of \( {\text{G}}_{{\text{ $ \alpha $ }}} \) gustducin was initially believed to be negatively coupled to cAMP. This differed with the positive coupling reported for sucrose taste. Indeed, direct measurements of cAMP after bitter taste verified that this second messenger declines with bitter stimulation [107]. Trubey et al. [102] also showed that umami-taste stimulation also causes a decrease in cAMP in rat taste buds, emphasizing the presence of transducin-like G proteins in taste cells. That \( {\text{G}}_{{\text{ $ \alpha $ }}} \) gustducin is intimately involved in bitter, umami, and sweet stimulation has been verified by showing that these taste qualities are significantly compromised in \( {\text{G}}_{{\text{ $ \alpha $ }}} \) gustducin knockout mice [108111].

Events downstream of changes in intracellular cAMP and that lead to transmitter release from taste cells have not been investigated in much detail. The original findings of Avenet et al. [103] mentioned above suggest that protein kinase A-mediated phosphorylation and inhibition of a potassium conductance could lead to membrane depolarization, thereby stimulating voltage-gated Ca2+ channels and allowing Ca2+ influx as a prelude to transmitter secretion. A somewhat different role for Ca2+ in taste cells is discussed next.

Involvement of the second messenger cAMP in sour and salty tastes, taste qualities that are not believed to be transduced by GPCRs, is not as well understood if it occurs at all.

Calcium as a second messenger in taste

The second major intracellular messenger stream that has been defined and which has been the focus of substantial attention in recent years is inositol 1,4,5-trisphosphate (IP3)-mediated release of intracellular Ca2+. The proteins and other mediators involved in this second messenger cascade have been identified in taste cells, including the discovery of a taste-specific isoform of phospholipase C (PLCβ2) [112], the presence of IP3R3 receptors in taste cells [113], the measurement of taste-evoked IP3 increases [114, 115], and the all-important observation that taste stimulation indeed evokes intracellular Ca2+ release [116, 117]. Notably, single-cell PCR experiments show that all the proteins in the PLCβ2–IP3–Ca2+ pathway are expressed in the same cell, the taste receptor cell [118, 119]. Lastly, mutant mice lacking PLCβ2 show compromised responses to sweet, umami, and bitter tastes [120].

That the PLCβ2–IP3–Ca2+ cascade is important in taste is undeniable, but it is not the exclusive pathway for taste. Dotson et al. [121] showed that PLCβ2 knockout mice have reduced responses to bitter-taste stimulation but not a complete absence of bitter taste. Clearly, pathways for chemosensory transduction that do not rely on PLCβ2 are present in taste cells.

Finally, another key participant in chemosensory transduction is the nonselective monovalent cation channel TRPM5. TRPM5 is expressed in a number of neuronal and epithelial cells, including taste bud cells and chemosensitive cells in the gut [122126]. Taste cells and some gut cells that express TRPM5 also express taste receptors and other elements of the PLCβ2-signaling stream [118, 120, 125]. Significantly, genetic knockout of TRPM5 renders mice less responsive to sweet, bitter, and umami tastes [120, 127].

Initially, it was believed that in response to taste stimulation, PLCβ2 directly or indirectly gated TRPM5 [120] or that depletion of Ca2+ from internal stores activated TRPM5 [128]. However, more recent findings show that TRPM5 channels are transiently and directly opened by small and rapid increases of intracellular Ca2+, such as those generated during taste stimulation as a consequence of the PLCβ2-signaling stream [123, 124, 126]. The net result of TRPM5 activation would be cation influx and membrane depolarization, presumably leading to neurotransmitter release. Interestingly, ion currents through TRPM5 are enhanced by temperature elevation in a physiologically relevant range, 15 to 35° [129]. This heat sensitivity of TRPM5 may explain the increase in sweet-taste intensity with temperature increases over this same range [129].

Figure 4 summarizes the two intracellular cascades of receptors and downstream effectors initiated by taste stimulation.
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Fig. 4

Taste stimulation for G protein-coupled taste receptors (e.g. sweet, bitter, umami) initiates two parallel streams of intracellular events. Several details are known for the PLCβ2-signaling stream (right). Considerably less is understood about the role of cyclic nucleotides in taste transduction (left), especially taste-evoked decreases in cAMP. The final events shown, depolarization leading to transmitter release, remain somewhat speculative

Information flow in the taste bud

A second intriguing aspect of peripheral mechanisms in taste is the finding that some degree of information processing occurs in taste buds. Researchers now recognize that there are functionally distinct populations of cells in mammalian taste buds, including separate populations of receptor cells and cells with synapses. Morphologically distinct taste cell types have been described from the earliest histological identification of taste cells up through electron microscopical studies (light microscopy: light cells, dark cells; electron microscopy: types I, II, III, and IV cells; reviewed [130]). Yet until of late, there has been no clear assignment of function to cell morphotype. Recent studies employing immunostaining suggested that proteins involved in the initial events of chemosensory transduction, such as taste GPCRs, \( {\text{G}}_{{\text{ $ \alpha $ }}} \) gustducin, PLCβ2, and TRPM5, are expressed in one type of cell (type II), but proteins involved in synapses and synaptic release of transmitters, such as SNAP-25 and neural cell adhesion molecule (NCAM), are present in a different type of taste cell (type III) [118, 119, 131, 132]. Functional imaging of isolated taste bud cells followed by single-cell PCR definitively revealed the existence of two separate classes of taste cells [118]. One class of taste cell responds to taste stimulation, indicating these are sensory receptor cells (formerly known as type II cells). Sensory receptor cells express taste GPCRs, PLCβ2, IP3R3, and TRPM5-proteins underlying known transduction cascades, reviewed above. The second class of cells is excited by depolarization with KCl but not by taste stimulation. These latter cells express a voltage-gated Ca2+ channel (namely, α1A), NCAM, and SNAP-25, i.e., proteins associated with synapses. These cells have been termed presynaptic cells (formerly known as type III cells) [118]. Presynaptic (type III) cells are the only cells in the taste bud that possess ultrastructural features of synapses (vesicles and synaptic thickenings). This raises the conundrum that the cells that directly respond to gustatory stimulation, i.e., taste receptor (type II) cells, are not the cells that make synapses, i.e., presynaptic (type III) cells. A possible resolution to this is described next.

Finger et al. [133] showed that adenosine triphosphate (ATP) is one of the neurotransmitters that activate gustatory primary afferent fibers. They found that mutant mice lacking P2X2 and P2X3 receptors had greatly reduced gustatory nerve and taste behavioral responses to glutamate (umami taste), sweeteners, and bitter compounds. These P2X purinoceptors had previously been localized to sensory afferent terminals innervating taste buds. Further, Finger et al. [133] measured tastant-evoked ATP secretion from pieces of lingual epithelium containing taste buds. Subsequently, Huang et al. [134, 135] used focal biosensor cells to demonstrate that individual sensory receptor (type II) cells in the taste bud secrete ATP when stimulated and that presynaptic (type III) cells release serotonin (5-HT). Further, Huang et al. [135] revealed that ATP also acts as a cell–cell mediator between receptor and presynaptic cells. Thus, ATP secretion from receptor cells during taste stimulation excites sensory afferent fibers and adjacent presynaptic cells concurrently (Fig. 5). In brief, chemosensory excitation of taste buds triggers release of ATP and serotonin alike.
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Fig. 5

Cell–cell communication within the mammalian taste bud. Taste receptor (type II) cells (left) secrete ATP in response to gustatory stimulation. ATP acts on sensory afferent fibers as well as on adjacent presynaptic (type III) cells (right), causing the latter to release serotonin (5HT). Consequently, gustatory stimulation of taste buds elicits ATP and serotonin release. Presynaptic cells form synapses, possibly serotonergic, with targets that have not yet been identified with confidence. Postsynaptic sites for 5HT may include other taste cells (paracrine activation), sensory afferent fibers, or unidentified postsynaptic targets, as shown above

Although evidence for a neurotransmitter role for ATP is firm, serotonin’s function in taste buds remains puzzling. In their report on purinergic neurotransmission in taste buds, Finger et al. [133] commented that mutant mice lacking one of the serotonin receptor subtypes, 5-HT3, showed no obvious taste-behavior abnormalities. Yet, there is substantial literature implicating serotonin as a taste bud neurotransmitter or neuromodulator [136]. The involvement of other receptor types on taste cells and nerves remains to be explored fully. For example, Kaya et al. [137] reported that 5-HT1A receptors are expressed on taste cells and proposed that serotonin is a paracrine transmitter at 5-HT1A receptors in taste buds. Serotonin may modulate the threshold of sensory receptor cells in the taste bud or alter membrane properties in taste bud cells [138, 139]. Indeed, a recent report from L. Donaldson’s laboratory shows selective serotonin re-uptake inhibitors alter human taste thresholds for sweet and bitter via a peripheral mechanism [140]. Further investigation of the role of serotonin in taste buds is clearly needed.

The finding that taste receptor cells secrete ATP onto sensory afferent fibers and onto nearby serotonergic taste cells may resolve the nagging problem that gustatory sensory receptor cells lack ultrastructural features associated with synapses, such as clusters of synaptic vesicles and presynaptic membrane thickenings. It seems that novel synaptic mechanisms, not involving exocytosis at well-defined synapses, underlie ATP secretion from sensory receptor cells. This arrangement might also explain why electrophysiological recordings from primary sensory axons often indicate that a single fiber can respond to two or more different taste qualities (e.g., sweet and bitter), and yet, taste receptor cells express only one type of GPCR, e.g., sweet or bitter, but not both. Namely, a single sensory afferent fiber could respond to ATP secreted from a small number of nearby taste receptor cells, which might not all express the same type of taste receptor. Further, ATP secreted from multiple taste receptor cells, including cells expressing different taste receptors, may be able to excite one presynaptic cell. That is, different types of receptor cells might converge onto one presynaptic cell. Thus, multiple-responsive taste cells that are reported from patch-clamp studies [141, 142] and Ca2+ imaging [79] would correspond to activity in presynaptic (type III) cells, not receptor (type II) cells.

Such an arrangement would explain the presence of taste cells that are “tuned” to one taste quality, e.g., sweet (receptor cells expressing T1R2+T1R3) alongside taste cells that respond to multiple qualities, e.g., sweet and bitter (presynaptic cells excited by ATP secreted from sweet-sensitive and bitter-sensitive receptor cells), as is observed. Similarly, sensory afferent fibers would respond predominantly to ATP secreted from receptor cells that they most closely abutted but might also be somewhat affected by ATP released from other nearby receptor cells. This would explain the existence of “sweet-best”, or “bitter-best” responsive taste afferents and not of exclusively sweet-, or exclusively bitter-responsive sensory afferents.

Type I taste bud cells (putative supporting cells) are likely to prevent ATP secreted from receptor cells from diffusing widely throughout the taste bud during taste stimulation. This would provide some degree of specificity for purinergic signaling between receptor cells and afferent axons. Type I cells ensheath surrounding cells with lamellar processes [143]. This wrapping alone might limit ATP diffusion within the taste bud. However, in addition, type I cells express a potent ecto-ATPase [144] that breaks down ATP. This ecto-ATPase would further limit ATP spread during taste stimulation. Conceivably, type I cells may even enwrap small clusters of receptor and presynaptic cells and their sensory afferent innervation, providing a protected environment for cell–cell communication among selected subgroups of taste cells (Fig. 6). Such an organization is speculation, however, and would require detailed 3-D reconstructions of taste buds at the ultrastructural level.
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Fig. 6

Schematic drawing of a mammalian taste bud illustrating hypothetical clustering of receptor (type II) and presynaptic (type III) cells for information coding. Left, nine representative taste cells (out of a total of 50 to 100, typical of taste buds) are depicted. A single sensory afferent axon is shown innervating a cluster of taste cells. For clarity, afferent innervation of other cells has been omitted. ATP secreted from receptor (type II) cells (light gray) excites afferent axons. ATP from receptor cells also stimulates presynaptic (type III) cells (dark gray). Middle, the cluster of cells contacting the afferent fiber is isolated for emphasis. Three receptor cells, each of which might express a similar taste GPCR, are shown for illustration. Activation of any of the receptor cells in the cluster would result in ATP secretion and excitation of the afferent fiber. ATP would also excite the presynaptic cell shown in the middle of the cluster. Right, a type I supporting cell ensheathing the cluster of taste cells is added. Type I cells express ecto-ATPase which would be expected to limit the diffusional spread of ATP throughout the taste bud, perhaps concentrating its actions on small clusters of cells as shown here. Even though each receptor cell expresses one type of taste GPCR, the information carried by the single sensory afferent (i.e., its “code”) might be ambiguous if not all the receptor cells in the cluster expressed identical taste GPCRs. Further, activity in the presynaptic (type III) cell would reflect stimulation of any of the nearby receptor cells. However, gustatory coding from the entire taste bud or multiple taste buds could be resolved if activity in numbers of sensory afferents were combined or averaged. Such a schema might explain how electrophysiological and functional imaging studies report the existence of taste cells that respond to multiple taste stimuli (here, presynaptic (type III) cells) alongside cells that respond only to one tastant (i.e., receptor (type II) cells). The schema would also explain the multiple taste sensitivity recorded from single afferent fibers

The presence of sensory afferent fibers that respond to multiple taste qualities has led to the concept of “cross-fiber” or combinatorial coding for gustatory information leaving the taste bud (reviewed [145]). The temporal pattern of action potentials excited by taste stimuli has been put forward as providing additional information about the nature of the chemical stimulus. However, most of the experimental effort aimed at this notion has been carried out by recording from CNS neurons involved in taste [146, 147]. A full understanding of taste coding in the CNS will require a better understanding of how the initial signals are specified in the peripheral sensory organs, the taste buds. As this review has indicated, considerable progress is currently being made along those lines. We have recently identified a novel transmitter release mechanism for ATP not involving vesicular exocytosis [151]. Receptor (type II) cells secrete ATP via pannexin 1 hemichannels during taste stimulation. Another group confirmed connexin and pannexin expression in lingual tissue and speculated that either or both may be involved in ATP release from taste cells [152].

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.Department of Physiology and BiophysicsMiller School of Medicine, University of MiamiMiamiUSA