Chronic CaMKII inhibition blunts the cardiac contractile response to exercise training
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- Kaurstad, G., Alves, M.N., Kemi, O.J. et al. Eur J Appl Physiol (2012) 112: 579. doi:10.1007/s00421-011-1994-0
Activation of the multifunctional Ca2+/calmodulin-dependent protein kinase II (CaMKII) plays a critical role modulating cardiac function in both health and disease. Here, we determined the effect of chronic CaMKII inhibition during an exercise training program in healthy mice. CaMKII was inhibited by KN-93 injections. Mice were randomized to the following groups: sham sedentary, sham exercise, KN-93 sedentary, and KN-93 exercise. Cardiorespiratory function was evaluated by ergospirometry during treadmill running, echocardiography, and cardiomyocyte fractional shortening and calcium handling. The results revealed that KN-93 alone had no effect on exercise capacity or fractional shortening. In sham animals, exercise training increased maximal oxygen uptake by 8% (p < 0.05) compared to a 22% (p < 0.05) increase after exercise in KN-93 treated mice (group difference p < 0.01). In contrast, in vivo fractional shortening evaluated by echocardiography improved after exercise in sham animals only: from 25 to 32% (p < 0.02). In inactive mice, KN-93 reduced rates of diastolic cardiomyocyte re-lengthening (by 25%, p < 0.05) as well as Ca2+ transient decay (by 16%, p < 0.05), whereas no such effect was observed after exercise training. KN-93 blunted exercise training response on cardiomyocyte fractional shortening (63% sham vs. 18% KN-93; p < 0.01 and p < 0.05, respectively). These effects could not be solely explained by the Ca2+ transient amplitude, as KN-93 reduced it by 20% (p < 0.05) and response to exercise training was equal (64% sham and 47% KN-93; both p < 0.01). We concluded that chronic CaMKII inhibition increased time to 50% re-lengthening which were recovered by exercise training, but paradoxically led to a greater increase in maximal oxygen uptake compared to sham mice. Thus, the effect of chronic CaMKII inhibition is multifaceted and of a complex nature.
KeywordsAerobic interval trainingKN-93Exercise training and cardiac functionCaMKII inhibition in healthy mice
The ubiquitous and multifunctional Ca2+/calmodulin-dependent protein kinase II (CaMKII) regulates a number of intracellular processes related to cellular contractility and nuclear gene expression, thereby controlling the pump function and growth of the heart. Some of the specific targets include the L-type Ca2+ channel, the sarcoplasmic reticulum (SR) Ca2+ release channel (ryanodine receptor 2, RyR2), phospholamban (PLB), Na+ and K+ channels making CaMKII a prominent regulator of excitation–contraction coupling (Couchonnal and Anderson 2008; Hashambhoy et al. 2010; Maier et al. 2007; Maier and Bers 2002). Furthermore, CaMKII can regulate nuclear gene expression on class II histone deacetylase (HDAC), which increases myocyte-enhanced factor 2 (Mef2)-regulated transcription (Erickson and Anderson 2008; Maier and Bers 2002, 2007; Maier et al. 2007; Sag et al. 2009). However, the functional consequences of CaMKII activation are still unclear, since heart failure is associated with increased CaMKII (Anderson 2005; Anderson et al. 1998; Couchonnal and Anderson 2008; Khoo et al. 2006; Vila-Petroff et al. 2007; Zhang et al. 2005); in fact, CaMKII may constitute a molecular switch between cardiac hypertrophy and failure (Zhang et al. 2005), and CaMKII activation may also precede arrhythmic events and contractile dysfunction, mainly because of its effect on the RyR2 (Anderson 2005; Ling et al. 2009; van Oort et al. 2010). On the other hand, improved contractile function after aerobic interval exercise training also associates with activated CaMKII, but in this scenario, the main effect is on the SR Ca2+ uptake due to phosphorylation of PLB. Interestingly, in mice with type-2 diabetes with chronically increased cardiac CaMKII and reduced cardiomyocyte contractile function and Ca2+ handling, aerobic interval exercise training resulted in reduced levels of CaMKII and improved cardiomyocyte contractile function and Ca2+ handling (Stolen et al. 2009). Recent studies have shown that CaMKII negatively regulates calcineurin activity (Khoo et al. 2006; MacDonnell et al. 2009), which in turn regulates mitochondrial respiration, and further complicates the role of CaMKII (Jiang et al. 2010; Wang et al. 2011).
The exact role of CaMKII activation in the heart remains unclear, as it associates with dysfunction, failure, and propensity for arrhythmias, but also with increased function under different circumstances such as exercise training. Based on the potential clinical value of chronic CaMKII inhibition (Khoo et al. 2006; Sag et al. 2009; Zhang et al. 2005) and the beneficial effect of exercise training on delaying cardiac dysfunction and correcting function after onset of heart disease (Stolen et al. 2009; Wisloff et al. 2002, 2007; Adams et al. 2005; Erbs et al. 2010; Mezzani et al. 2008; Hambrecht et al. 2000; Anderson et al. 1998; Kemi et al. 2007; Zhang et al. 2005) two scenarios that may mutually oppose each other, determining the effect of chronic CaMKII inhibition on the response to exercise training would be of considerable interest. Therefore, we aimed to study the effect of chronic CaMKII inhibition during the course of an exercise training program in healthy mice. We hypothesized that chronic CaMKII inhibition would abolish the normal exercise training response in the hearts.
Materials and methods
Mice and CaMKII inhibition
Eighteen female C57 BL/6J mice (Møllegaard Breeding Center, Lille Skensved, Denmark) 18–20 g, 8 weeks of age at inclusion were randomized into four groups: (1) sham control sedentary, (2) sham control exercise training, (3) KN-93 sedentary, and (4) KN-93 exercise training. KN-93 (2-[N-(2-hydroxyethyl)-N-(4 methoxybenzenesulfonyl)]amino-N-(4-chlorocinnamyl)-N-methylbenzylamine; Alexis-Biochemical, Enzo Life Science, PA, USA) diluted in dimethyl sulfoxide (DMSO, Sigma-Aldrich, St. Louis, MO, USA, 2 mg KN-93/1 mL DMSO, 10 μmol/kg (Zhang et al. 2005)) was intraperitoneally injected daily after the exercise training session (for those that exercise trained), throughout the experimental period. In a previous study, Zhang et al. (2005) observed dose-dependent CaMKII inhibition by KN-93, in which daily injections of 10 μmol/kg KN-93 produced similar effect on left ventricular contraction measurements as the genetically CaMKII-inhibited AC3:I mice (Zhang et al. 2005; Khoo et al. 2006). Sham control mice received similar amounts of DMSO daily. The Norwegian council for Animal Research approved the study, which was in accordance with the Guide for the Care and Use of Laboratory Animals (National Institutes of Health Publications No. 85–23, revised 1996).
Exercise training was performed as aerobic interval training for 6 weeks, 5 days/week, on an inclined (25°) treadmill, each session starting with a 10-min warm-up at 50–60% of maximal oxygen uptake (VO2max), whereupon exercise then alternated between 4- and 2-min intervals at 85–90 and 50–60% of VO2max, respectively for 60 min. VO2max was assessed during a treadmill running test to exhaustion in a metabolic chamber. After a warm-up period of about 10 min, treadmill band velocity was increased by 0.03 m/s for every second minute until mice were not able to maintain the treadmill velocity (Hoydal et al. 2007; Kemi et al. 2002). To maintain the exercise intensity throughout the experimental period, VO2max was tested at the beginning of each week in addition to pre- and post-tests. This exercise protocol has previously been proven efficient for inducing exercise and cardiovascular adaptations in both the clinical and experimental studies (Tjonna et al. 2008; Hoydal et al. 2007; Kemi et al. 2002, 2005; Wisloff et al. 2001; Stolen et al. 2009).
High-resolution echocardiography (Vevo 770, VisualSonics, Toronto, Canada) using a single-element mechanical transducer with a center frequency of 30 MHz was performed on self-breathing mice exposed to a mixture of 2% isoflurane and 98% oxygen. A two-dimensional long-axis view was used to visualize both ventricles, the ascending aorta, and the right ventricular outflow tract by placing the ultrasound transducer on the left parasternal position, whereas a short-axis view was used to visualize systolic and diastolic movement of both ventricles by placing the transducer horizontally above the heart. Left ventricular end-diastolic diameter (LVEDD), left ventricular end-systolic diameter (LVESD), interventricular septum (IVST) and posterior wall thickness (PWT) were recorded with M-mode echocardiography, which allowed calculation of the fractional shortening (% = [(LVEDD − LVESD)/LVEDD] × ) as an index of systolic left ventricular function. The following equation was used to calculate LV mass (mg) = [(LVEDD + IVST + PWT)3 − LVEDD3] × 1.055, where 1.055 (mg/mm3) is the density of myocardium. IVST and PWT were measured at end diastole.
Cardiomyocyte isolation, contractile function, Ca2+ measurements and cell size
Left ventricular cardiomyocytes were isolated as previously described using a Hepes-based solution (Mitra and Morad 1981; Guatimosim et al. 2001). Exercised hearts were excised 24 h after the last exercise session. A mixture of 3–4% isoflurane and 96–97% oxygen were used to anesthetize the mice, whereupon the heart was heparinized and removed. Until it was connected to the aortic cannula on a standard Langendorff retrograde perfusion system, the hearts were kept on ice cold Hepes solution. The hearts were cleaned with a Hepes solution containing EGTA (Sigma-Aldrich Corp., Missouri, USA) before the perfusion was switched to Hepes solution with collagenase (Worthington, Lakewood, USA), and perfusion was continued for approximately 10 min (3.5 ml/min). After the hearts were taken down, left ventricles were carefully cut off and gently shaken for 2 min before the non-digested tissue was filtered out (nylon mesh, 250 μm). Further, cells were stepwise exposed to increasing Ca2+ levels (initially 0.05 mM). The isolated cells were centrifuged (30 s, 600 rpm) before solution was switched to 1.2 mM Hepes-based solution. Fura-2/AM-loaded (2 μmol/l, Molecular Probes, Eugene, OR, USA) cardiomyocytes were field stimulated by bipolar electrical pulse at 1 Hz, on an inverted epifluorescence microscope (Nikon TE-2000E, Tokyo, Japan), whereupon cell shortening was recorded by video-based myocyte sarcomere spacing (SarcLen™, IonOptix, Milton, MA, USA) and intracellular Ca2+ concentration was measured by fluorescence after excitation by alternating 340 and 380 nm wavelengths (F340/380 ratio) (Optoscan, Cairn Research, Kent, UK). Cells with a clear visual intracellular structure that were able to follow stimulation frequency were included for the experiment. The total number of cardiomyocytes included for all analysis in the different groups was: sham sedentary 8, sham exercise 20, KN-93 sedentary 5, and in KN-93 exercise 9. During the stimulation protocol, cells were kept in 1.2 mM Ca2+ HEPES-based solution at 37°C. Cell size was measured with Nikon Eclipse E400 Microscope with a DSFil camera (Nikon NIS-Elements Basic Research Version 3.00 software, Nikon Instruments Inc., Melville, NY, USA). Cardiomyocyte size was measured on 80–120 cardiomyocytes per group.
A one-way ANOVA with LSD post hoc test was used to detect significant differences between groups. For within-group differences from pre- to post-test, a paired sample T test was used. Statistical significance level was set to p < 0.05.
VO2max and exercise capacity
Two-dimensional echocardiography measurements
441 ± 21.3
450 ± 36.2
478 ± 18.0
447 ± 36.4
23.7 ± 4.0
25.2 ± 1.4
20.2 ± 5.7
25.4 ± 6.0
10.4 ± 1.8
11.3 ± 1.4
9.7 ± 2.7
11.3 ± 2.3
3.7 ± 0.2
3.7 ± 0.2
3.5 ± 0.3
3.8 ± 0.2
2.8 ± 0.3
2.5 ± 0.3
2.6 ± 0.2
2.7 ± 0.1
LV mass (mg)
96 ± 11.7
94 ± 8.7
85 ± 23.5
99 ± 23.0
25 ± 4.5
32 ± 5.2*
28 ± 3.2
30 ± 1.6
0.69 ± 0.07
0.7 ± 0.09
0.75 ± 0.09
0.7 ± 0.11
0.74 ± 0.06
0.66 ± 0.14
0.7 ± 0.12
0.68 ± 0.06
Cardiac contractile function
Chronic CaMKII inhibition by KN-93 injections induced a reduction of the cardiomyocyte ability to re-lengthen (25% increased time to 50% re-lengthening; Fig. 4c, p < 0.05) after twitch contractions. This was at least partly explained by the 16% increase in the Ca2+ transient decay time (Fig. 4d, p < 0.05). Exercise training normalized cardiomyocyte re-lengthening and Ca2+ transient decay times to levels comparable to sedentary sham mice, and the response to exercise training was not different between sham and KN-93 mice. In particular, exercise training decreased the re-lengthening time by 12% (p < 0.05) and 16% (p < 0.05) in sham and KN-93 mice, respectively, which was linked to comparable exercise training-induced changes in the Ca2+ transient decay times (Fig. 4c, d).
Comparison of effects of CaMKII inhibition on exercise training response
Aerobic exercise capacity
LV fractional shortening
Cardiomyocyte Ca2+ handling
Ca2+ transient amplitude
Time to 50% re-lengthening
Time to 50% Ca2+ decay
Given that (1) experimental trials (Anderson 2005; Zhang et al. 2005; Grimm and Brown 2010) have raised the possibility that systemic CaMKII inhibition may be a viable and effective strategy for the treatment of heart disease, and (2) exercise training in both experimental (Rose et al. 2007; Wisloff et al. 2002; Stolen et al. 2009) and clinical (Adams et al. 2005; Erbs et al. 2010; Hambrecht et al. 2000; Mezzani et al. 2008; Wisloff et al. 2007) trials show improved heart function in similar scenarios of heart disease, we aimed to test the potential value of combining those strategies; first in normal healthy mice. The reasoning for the latter is that exercise training and CaMKII also interact in the heart, as the inotropic effects of exercise training are at least partly modulated by an exercise training-induced increase in CaMKII activity (Kemi et al. 2007). This complicates the combination scenario as CaMKII seems to act as a two edged sword. In fact, CaMKII contributes to decompensate pathologic hypertrophy to heart failure, mainly by its action on the RyR2 to increase SR Ca2+ leak (Sag et al. 2009; Ling et al. 2009). Thus, the possibility arises that chronic CaMKII inhibition and exercise training may oppose each other, though it remains to be studied.
As such, this is the first study to introduce the combination of chronic CaMKII inhibition by daily KN-93 injections and aerobic interval exercise training. The main finding was that chronic CaMKII inhibition blunted several aspects of the response patterns of cardiomyocyte contractility, and intracellular Ca2+ handling to exercise training, but it did not fully prevent such adaptations. Moreover, CaMKII inhibition also enhanced exercise training-induced improvements on VO2max and aerobic exercise capacity, whereas it did not affect either of them in sedentary mice.
Mechanisms of exercise training-induced adaptation
The present study provides several mechanistic clues as to the response to exercise training. First, it shows that while CaMKII activation contributes to the cardiac improvements after exercise training (Kemi et al. 2007), other mechanisms must also contribute, as CaMKII inhibition only partly blunted the exercise response. Whether those mechanisms naturally co-exist or occur to compensate for the loss of CaMKII activation remains unknown.
Second, the finding that VO2max and exercise capacity responded more to exercise training in the presence of the chronic CaMKII inhibition compared to the absence thereof indicates that CaMKII also may negatively regulate exercise adaptation in peripheral organs. While this requires further investigation, it is plausible all the while it is ubiquitous and exists in most, if not all, cellular systems (Hudmon and Schulman 2002; Chin 2004).
Third, the opposite effects of exercise training on VO2max and exercise capacity on the one side, and cardiac inotropy and hypertrophy on the other side in the face of CaMKII inhibition highlights that other organs are important in determining VO2max (Bassett and Howley 2000; Coffey and Hawley 2007). In particular, chronic exercise training elicits resistance to muscle fatigue through metabolic responses including mitochondrial biogenesis, increased oxidative capacity, and alterations in gene and protein expression that ultimately leads to phenotype changes that support endurance-type activity (Rose et al. 2007; Benziane et al. 2008; Chin 2004; Coffey and Hawley 2007; Bassett and Howley 2000). The exact mechanism of this rather surprising observation is not known; however, the interaction between CaMKII and calcineurin is a possible candidate. CaMKII modulation of calcineurin signaling is released by CaMKII inhibition. Calcineruin modulates exercise-induced skeletal muscle phenotypes and enhances exercise capacity through increase in mitochondrial oxidative function and energy substrate storage in skeletal muscles (Wang et al. 2011; Jiang et al. 2010). This should be elucidated in future studies.
Cardiac contractile capacity, Ca2+ handling, and CaMKII
Both in vivo and cellular fractional shortening were unaffected by KN-93-induced inhibition of CaMKII in sedentary mice, whereas the expected training-induced increase in both whole-heart and cellular fractional shortening was blunted by the chronic KN-93 treatment. However, chronic CaMKII inhibition did reduce the rate of diastolic cellular relaxation in sedentary mice, which was explained by slower intracellular Ca2+ removal (longer time to 50% Ca2+ transient decay) and higher systolic Ca2+ levels. Exercise training normalized Ca2+ removal times, and in contrast to fractional shortening, the effect was not blunted by CaMKII inhibition. These results are in accordance with Kemi et al. (2007), who found that acute pre-incubation, with AIP (comparable CaMKII inhibitor) in isolated cardiomyocytes also blunted the exercise training-induced improvements in cellular contractility. Together, these results suggest that CaMKII at least partly modulates the exercise training-induced improvements in cardiac contractility, excitation–contraction coupling, and intracellular Ca2+ handling (Kemi et al. 2007; Stolen et al. 2009). The accumulated evidence also suggests that this modulation occurs via targeting of several loci of excitation–contraction coupling and Ca2+ handling (Stolen et al. 2009). Indeed, fractional shortening is dependent on both SR Ca2+ release and myofilaments Ca2+ sensitivity, where the latter may explain the dissociation between fractional shortening and the intracellular Ca2+ transient amplitude in the present study, as shown directly by others after exercise training (de Waard et al. 2007; Diffee et al. 2001). However, in our experiments we used unloaded myocytes where tension development and basal sarcomere length were not considered.
Current results together with the previous studies (Anderson 2005; Anderson et al. 1998; Couchonnal and Anderson 2008; Erickson and Anderson 2008; Kemi et al. 2007; Khoo et al. 2006; Maier and Bers 2002; Maier et al. 2007; Sag et al. 2009; Vila-Petroff et al. 2007; Zhang et al. 2005) suggest that CaMKII is a versatile kinase that may shift cardiac function into different and also opposite phenotypes. In healthy mice, it appears that a controlled increase in the CaMKII activity increases cardiac contraction (Kemi et al. 2007); that CaMKII inhibition reduces cardiac contraction, and that these effects mainly occur because CaMKII inhibition reduces SR Ca2+ uptake via SERCA2a. Therefore, it is also possible that the depression of cardiomyocyte contractile parameters by chronic CaMKII inhibition observed here might have progressed to a global dysfunction and a failure if CaMKII inhibition was continued. As such, maintained CaMKII seems to be important for normal cardiomyocyte function in healthy mice. In contrast, the cardiac dysfunction that also has been linked to increased CaMKII activity (Ai et al. 2005; Couchonnal and Anderson 2008; Maier et al. 2007; Zhang and Brown 2004) has mainly been attributed to hypersensitization of the RyR2 to luminal Ca2+ with subsequent diastolic SR Ca2+ leak and a shift of Ca2+ out of the cell; a scenario that may lead to contractile dysfunction and increased propensity for arrhythmic events (Sag et al. 2009; Ai et al. 2005; Grimm and Brown 2010; Khoo et al. 2006; Ling et al. 2009; Stolen et al. 2009; Wu et al. 2002), in which CaMKII inhibition or reduction would become particularly beneficial (Kemi et al. 2007; Laurita and Rosenbaum 2008; Li et al. 2006; Vila-Petroff et al. 2007; Yang et al. 2006; Zhang et al. 2005). In a recent modulation study, Hashambhoy et al. (2010) report that inhibition of CaMKII phosphorylation of the L-type Ca2+ channel rather than the RyR2 is more effective in modulating diastolic RyR2 flux. Thus, a pharmacological approach of CaMKII inhibition in the heart should also target the L-type Ca2+ channel in order to prevent or treat cardiac dysfunction and disease. Whether this will be feasible remains to be investigated. KN-93 inhibits CaMKII by competing for the calmodulin binding site, and has been widely used to implicate roles of CaMKII in Ca2+ handling (Sumi et al. 1991). KN-93 is not heart-specific and is known to have other actions than CaMKII inhibition (Anderson et al. 1998; Gao et al. 2006). Previous studies have observed that KN-93 might inhibit L-type Ca2+ current independent of CaMKII (Anderson et al. 1998; Gao et al. 2006). This negative effect of KN-93 on intracellular Ca2+ levels are balanced by inhibition of voltage-dependent potassium currents which enhance Ca2+ entry via L-type Ca2+ channel (Anderson et al. 1998; Rezazadeh et al. 2006; Ledoux et al. 1999). The lack of organ specificity of KN-93 is a limitation of this study, and interpretations should be considered with caution. We did, however, control for non-cardiac side effects of chronic KN-93 injections by the pathological examinations of the vital organs after euthanasia in mice.
Echocardiography measurements observed a trend for decreased LV mass in sedentary KN-93 treated mice, which was normalized by exercise-induced hypertrophy, and cardiomyocyte length and width increased significantly less in KN-93-treated mice compared to sham-treated mice. These results are somewhat similar to the observations of Zhang et al. (2005), with myocardial infarction as the physiological stressor, who reported that cardiomyocyte transverse cross-sectional area and heart weight were comparably smaller after genetic inhibition of CaMKII than in control cardiomyocytes. Furthermore, Ramirez et al. (1997) reported that pre-treatment with KN-93 blocked hypertrophic responses to the hypertrophy developed by pressure overload or endothelin-1 (Zhang et al. 2004; Zhu et al. 2000). Our observation suggests that CaMKII may also modulate cardiomyocyte growth in response to exercise training (physiological hypertrophy) and not only in response to the pathologic conditions.
This study indicates the importance of maintaining normal CaMKII activity in cardiomyocytes of healthy individuals, also because it positively modulates inotropic/lusitropic responses to exercise training. However, targeting CaMKII by selective inhibitors has recently been suggested to correct cardiac dysfunction and prevent decompensation and progression of heart disease; a clinical scenario that also is targeted by exercise training. This study indicates that a combination strategy of CaMKII inhibition and exercise training may be feasible for the purpose of attenuating heart disease, although this does present a complex scenario that may also reduce some of the beneficial effects of exercise training, especially if CaMKII inhibitors cannot be closely tuned into localized subcellular targets that mainly cause the cardiac dysfunction.
The small molecule inhibitor KN-93 has potential non-specific effects other than CaMKII inhibition which were not controlled for; this is a limitation of the present study. The pathological examinations were done to rule out effects on the results from any of the other vital organs due to KN-93 or DMSO injections.
We thank Trine Skoglund for excellent laboratory assistance. The study was supported by grants from the K.G. Jebsen Foundation; the Blix Foundation for the Promotion of Medical Science (GK), and the Norwegian Research Council Funding for Outstanding Young Investigators (UW), and the British Heart Foundation (OJK).
Conflict of interest
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