Original Article

European Journal of Applied Physiology

, Volume 110, Issue 5, pp 905-913

Pentraxin3 and high-sensitive C-reactive protein are independent inflammatory markers released during high-intensity exercise

  • Toshiaki NakajimaAffiliated withDepartment of Ischemic Circulatory Physiology, KAATSU Training, The University of Tokyo Email author 
  • , Miwa KuranoAffiliated withDepartment of Ischemic Circulatory Physiology, KAATSU Training, The University of Tokyo
  • , Takaaki HasegawaAffiliated withDepartment of Ischemic Circulatory Physiology, KAATSU Training, The University of Tokyo
  • , Haruhito TakanoAffiliated withDepartment of Ischemic Circulatory Physiology, KAATSU Training, The University of Tokyo
  • , Haruko IidaAffiliated withDepartment of Ischemic Circulatory Physiology, KAATSU Training, The University of Tokyo
  • , Tomohiro YasudaAffiliated withDepartment of Ischemic Circulatory Physiology, KAATSU Training, The University of Tokyo
  • , Taira FukudaAffiliated withDepartment of Cardiovascular Medicine, The University of Tokyo
  • , Haruhiko MadarameAffiliated withDepartment of Ischemic Circulatory Physiology, KAATSU Training, The University of Tokyo
  • , Kansei UnoAffiliated withDepartment of Cardiovascular Medicine, The University of Tokyo
    • , Kentaro MeguroAffiliated withDepartment of Ischemic Circulatory Physiology, KAATSU Training, The University of Tokyo
    • , Taro ShigaAffiliated withDepartment of Cardiovascular Medicine, The University of Tokyo
    • , Mina SagaraAffiliated withThe Perseus Proteomics Inc
    • , Taiji NagataAffiliated withDepartment of Respiratory Medicine, The University of Tokyo
    • , Koji MaemuraAffiliated withDepartment of Cardiovascular Medicine, The University of Tokyo
    • , Yasunobu HirataAffiliated withDepartment of Cardiovascular Medicine, The University of Tokyo
    • , Tatsuya YamasobaAffiliated withDepartment of Otolaryngology and Head and Neck Surgery, Faculty of Medicine, The University of Tokyo
    • , Ryozo NagaiAffiliated withDepartment of Cardiovascular Medicine, The University of Tokyo

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Abstract

High-intensity exercise shares similarities with acute phase responses of inflammatory diseases. We investigated the influences of acute exercise on inflammatory markers, plasma pentraxin3 (PTX3) and serum high-sensitive C-reactive protein (CRP) (hsCRP). Nine healthy male subjects (41 ± 3 years old) participated. Each subject performed three types of exercise; ergometer exercise at 70% workload of anaerobic threshold (AT) for 30 min (70% AT exercise), peak ergometer exercise (peak EX, 20 watt increase/min until fatigue) and resistance exercises of 70% 1 RM (70% RE) until exhaustion. We measured plasma PTX3, serum hsCRP, lactate, noradrenaline (NOR), white blood cells (WBC), interleukin-6 (IL-6) and myeloperoxidase (MPO), a marker of neutrophil degranulation. The effects of exercise on intracellular PTX3 and MPO in neutrophils were also investigated, by using flow cytometry analysis. Circulating PTX3 and hsCRP significantly increased immediately after 70% RE and peak EX, while they did not increase after 70% AT exercise. The exercise-induced fold increase in PTX3 and hsCRP relative to the resting level was positively correlated with the changes in WBC, NOR, lactate and MPO. The exercise-induced fold increase in IL-6 was positively correlated with that in NOR, but not with that in PTX3 and hsCRP. Neutrophils isolated immediately after 70% RE, but not 70% AT exercise, exhibited lower mean fluorescence for PTX3 and MPO than those from pre-exercise blood. These results provide the evidence that high-intensity exercises significantly increase circulatory PTX3 as well as hsCRP. The release from peripheral neutrophils is suggested to be involved in the exercise-induced plasma PTX3 increase.

Keywords

Pentraxin3 High-sensitive CRP Inflammation Neutrophil Interleukin-6 Resistance exercise High-intensity exercise Myeloperoxidase Flow cytometry