European Journal of Applied Physiology

, Volume 110, Issue 4, pp 695–701

Cell-free plasma DNA and purine nucleotide degradation markers following weightlifting exercise

  • Johanna Atamaniuk
  • Claudia Vidotto
  • Markus Kinzlbauer
  • Norbert Bachl
  • Beate Tiran
  • Harald Tschan
Original Article

DOI: 10.1007/s00421-010-1532-5

Cite this article as:
Atamaniuk, J., Vidotto, C., Kinzlbauer, M. et al. Eur J Appl Physiol (2010) 110: 695. doi:10.1007/s00421-010-1532-5

Abstract

The present study aimed to investigate the acute effects of a single bout of high-intensive strength training on the production of cell-free plasma DNA (cf-DNA), as well as on the degradation of purine nucleotides as assessed by the concentration of xanthine (XA) and hypoxanthine (HX) in urine and serum. Twelve trained weightlifters performed six sets of six lifting exercises with 90–95% of the one repetition maximum. Blood samples and urine were obtained 1 h before training, immediately after finishing the exercise session and following 2 h of recovery. Cf-DNA, HX, and XA (in serum) significantly increased (P < 0.05–P < 0.001) immediately after heavy lifting exercise when compared with baseline levels, and significantly decreased (P < 0.05–P < 0.001) after 2 h of recovery. These results indicate that, cf-DNA and oxypurines might be relevant biomarkers for cellular damage, mechanical, energetic, and/or ischemic stress in context with exercise.

Keywords

Cell-free plasma DNA Oxypurines Muscle damage Resistance exercise Weightlifting 

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Johanna Atamaniuk
    • 1
  • Claudia Vidotto
    • 2
  • Markus Kinzlbauer
    • 3
  • Norbert Bachl
    • 3
  • Beate Tiran
    • 4
  • Harald Tschan
    • 3
  1. 1.Institute of Laboratory DiagnosticsKaiser Franz Josef HospitalViennaAustria
  2. 2.BKW Laboratory Diagnostics Inc.ViennaAustria
  3. 3.Department of Sport and Exercise Physiology, Center of Sport ScienceUniversity of ViennaViennaAustria
  4. 4.Department of Laboratory MedicineUniversity of GrazGrazAustria