Strenuous exercise increases late outgrowth endothelial cells in healthy subjects

  • Daniel Thorell
  • Mats Borjesson
  • Pia Larsson
  • Erik Ulfhammer
  • Lena Karlsson
  • Smita DuttaRoy
Original Article

DOI: 10.1007/s00421-009-1144-0

Cite this article as:
Thorell, D., Borjesson, M., Larsson, P. et al. Eur J Appl Physiol (2009) 107: 481. doi:10.1007/s00421-009-1144-0

Abstract

Endothelial progenitor cells (EPCs) and late outgrowth endothelial cells (OECs) seem to play an important role in vessel formation. While EPCs seem to exert their function mainly through a paracrine effect, the OECs can develop into mature endothelial cells and form tubular structures. Exercise is known to increase angiogenic factors that can mobilize EPCs; however, the effect on OECs is not known. We investigated the response to a single session of strenuous exercise on OECs, vascular endothelial growth factor (VEGF) and inflammatory cell levels in the healthy. Eleven healthy subjects performed 1 h of spinning exercise. Blood samples were collected at 1, 6, 24 and 48 h post-exercise for cell culture and biochemical analysis. OEC colonies doubled one hour after the spinning session (baseline 4.5 ± 4.3 vs. 9.0 ± 3.7, P < 0.05). Serum VEGF increased from 194 ± 107 pg/ml at baseline to 224 ± 111 pg/ml after 1 h, p = ns and neutrophilic granulocytes increased from 3.73 ± 1.38 at baseline to 9.08 ± 10.5 at 1 h (P < 0.01). The increased levels of OECs, VEGF and neutrophilic granulocytes declined gradually at the following time points. VEGF levels and neutrophilic granulocytes were highly correlated to OEC levels, r = 0.903 (VEGF) and r = 0.85 (neutrophilic granulocytes), respectively. Strenuous physical activity increases OEC colonies and is correlated to serum VEGF and neutrophilic granulocytes levels. An acute exercise-induced inflammatory response might be responsible for the VEGF release and subsequent increase of OECs. The clinical importance of these findings remains to be elucidated.

Keywords

Endothelial progenitor cellsExerciseVascular endothelial growth factorOutgrowth endothelial cellsInflammation

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Daniel Thorell
    • 1
  • Mats Borjesson
    • 1
  • Pia Larsson
    • 1
  • Erik Ulfhammer
    • 1
  • Lena Karlsson
    • 1
  • Smita DuttaRoy
    • 1
  1. 1.Department of Emergency and Cardiovascular MedicineSahlgrenska University Hospital/Östra, Institute of Medicine, Sahlgrenska Academy at Göteborg UniversityGöteborgSweden