Original Article

European Journal of Applied Physiology

, Volume 107, Issue 2, pp 227-233

First online:

Effect of 6-day intense Kendo training on lymphocyte counts and its expression of CD95

  • Yuko TanimuraAffiliated withGraduate School of Comprehensive Human Sciences, University of Tsukuba
  • , Michihiro KonAffiliated withGraduate School of Comprehensive Human Sciences, University of Tsukuba
  • , Kazuhiro ShimizuAffiliated withGraduate School of Comprehensive Human Sciences, University of Tsukuba
  • , Fuminori KimuraAffiliated withGraduate School of Comprehensive Human Sciences, University of Tsukuba
  • , Ichiro KonoAffiliated withGraduate School of Comprehensive Human Sciences, University of Tsukuba
  • , Ryuichi AjisakaAffiliated withGraduate School of Comprehensive Human Sciences, University of Tsukuba Email author 

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Abstract

This study examines the effects of 6-day intensive training on lymphocyte counts and their expression of CD95. Eight healthy Kendo athletes underwent 6-day Kendo training of about 310 min each day. Blood samples were collected at 2 weeks before (PRE), the first day (Day 1), third day (Day 3), fifth day (Day 5), and 1 week after the training period (POST) to determine lymphocyte counts and CD95 expression on CD95 lymphocytes (CD4+, CD8+) using flow cytometry. The total lymphocyte counts were significantly lower at Day 3 than at PRE. The CD8+ cell counts were significantly lower at Day 3 than at PRE. The percentage of CD95+ lymphocytes was significantly higher at Day 1 and Day 3 than at PRE. The percentage of CD8+CD95+ cells did not change significantly. The total lymphocyte counts decreased and a concomitant increase of CD95+ lymphocyte was observed, whereas the decrease in CD8+ cell counts was not associated with the increase in CD8+CD95+ cells. Therefore, short-term high-intensity exercise induced a decrease in the T lymphocyte counts without increasing in CD95+ expression.

Keywords

Intensified training Apoptosis CD95 Lymphocytopenia