European Journal of Applied Physiology

, Volume 104, Issue 4, pp 711–717

Effects of ultra-marathon on circulating DNA and mRNA expression of pro- and anti-apoptotic genes in mononuclear cells

  • Johanna Atamaniuk
  • Karl M. Stuhlmeier
  • Claudia Vidotto
  • Harald Tschan
  • Astrid Dossenbach-Glaninger
  • Mathias M. Mueller
Original Article

DOI: 10.1007/s00421-008-0827-2

Cite this article as:
Atamaniuk, J., Stuhlmeier, K.M., Vidotto, C. et al. Eur J Appl Physiol (2008) 104: 711. doi:10.1007/s00421-008-0827-2

Abstract

We investigated the effects of an ultra-marathon on cell-free plasma DNA as well as on mRNA expression of pro-apoptotic (Bax, Bad), anti-apoptotic (Bcl-2) and cell-protective (Hsp70, Hsp27 and Hsp32) genes in mononuclear blood cells (MNCs). Blood samples were drawn from 14 athletes before and immediately after 6-h run. In addition, blood samples were also collected and analyzed 2 and 24 h after the end of the run. Levels of plasma DNA were significantly increased immediately after the marathon (< 0.001) and were still higher 2 h later (P < 0.005), but significantly lower than those immediately after the race (< 0.05). Cell-free plasma DNA returned to pre-race levels 24 h after the run. mRNA expressions of Hsp70, Hsp32 and Bax significantly increased in MNCs after the race, whereas Hsp27 and Bad mRNA expression levels showed no significant changes. Bcl-2 expressions decreased immediately after the race (< 0.001), but increased in the 24 h later (< 0.05). We conclude that apoptotic ladders of cell-free DNA following exhaustive exercise originate from apoptotic cells and that not only skeletal muscle cells but also leukocytes contribute to this phenomenon.

Keywords

Cell-free plasma DNA Apoptotic genes Anti-apoptotic genes Ultra-marathon 

Supplementary material

421_2008_827_MOESM1_ESM.pdf (208 kb)
Reference DUMKE CL et al. (PDF 208 kb)

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Johanna Atamaniuk
    • 1
  • Karl M. Stuhlmeier
    • 2
  • Claudia Vidotto
    • 1
  • Harald Tschan
    • 3
  • Astrid Dossenbach-Glaninger
    • 4
  • Mathias M. Mueller
    • 1
  1. 1.Institute of Laboratory DiagnosticsKaiser Franz Josef HospitalViennaAustria
  2. 2.Ludwig Boltzmann Institute for RheumatologyViennaAustria
  3. 3.Department of Sports and Exercise PhysiologyInstitute of Sports Science, University of ViennaViennaAustria
  4. 4.Department of Laboratory MedicineRudolfstiftung HospitalViennaAustria

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