Histochemistry and Cell Biology

, Volume 111, Issue 1, pp 49–54

Co-localization of synaptophysin with different neuroendocrine hormones in the human gastrointestinal tract

  • G. M. Portela-Gomes
  • Mats Stridsberg
  • Henry Johansson
  • Lars Grimelius
ORIGINAL PAPER

DOI: 10.1007/s004180050332

Cite this article as:
Portela-Gomes, G., Stridsberg, M., Johansson, H. et al. Histochemistry (1999) 111: 49. doi:10.1007/s004180050332

Abstract

 Colocalisation of synaptophysin has been studied in different neuroendocrine cell types in histologically normal mucosa from human gastrointestinal tract (corpus, antrum, duodenum, ileum and colon) using double-immunofluorescence stainings. Numerous synaptophysin immunoreactive cells were seen in the antrum, while a smaller number were found in the intestinal tract. Synaptophysin immunoreactivity was strong in the antrum but weak in the intestine. In the intestinal colocalisation studies the synaptophysin immunoreactivity was enhanced by using the tyramide amplification method. Synaptophysin and chromogranin A were colocalised but the latter occurred mainly basally, whereas synaptophysin was found to occur diffusely throughout the cytoplasm. Synaptophysin immunoreactivity occurred in the serotonin cells throughout the gastrointestinal tract, and in the antral gastrin and somatostatin cells. In the intestinal tract only a small fraction of somatostatin, gastrin, cholecystokinin, enteroglucagon, enteroglucagon/ peptide tyrosine tyrosine displayed synaptophysin immunoreactivity. In the gastrointestinal tract (except the antrum), chromogranin A is a better general neuroendocrine marker than synaptophysin. The functional role of synaptophysin is unclear but it may be involved in the intracellular transport and release of hormones. Based on the distribution background of synaptophysin, it seems to be of greater importance in the antrum than in the intestinal tract as a whole.

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • G. M. Portela-Gomes
    • 1
  • Mats Stridsberg
    • 3
  • Henry Johansson
    • 4
  • Lars Grimelius
    • 1
  1. 1.Department of Pathology, University Hospital, S-75185 Uppsala, Sweden e-mail:portela@patologi.uu.se Fax +46–18–502172SE
  2. 2.Department of Medicine II, University Hospital of Santa Maria, Lisbon, PortugalPT
  3. 3.Department of Clinical Chemistry, University Hospital, Uppsala, SwedenSE
  4. 4.Department of Surgery, University Hospital, Uppsala, SwedenSE

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