Histochemistry and Cell Biology

, Volume 110, Issue 5, pp 467–476

Phospholipid rearrangement of apoptotic membrane does not depend on nuclear activity

  • Filippo Renò
  • Sabrina Burattini
  • Stefano Rossi
  • Francesca Luchetti
  • Marta Columbaro
  • Spartaco Santi
  • Stefano Papa
  • E. Falcieri
ORIGINAL PAPER

DOI: 10.1007/s004180050308

Cite this article as:
Renò, F., Burattini, S., Rossi, S. et al. Histochemistry (1998) 110: 467. doi:10.1007/s004180050308

Abstract

 The behaviour of plasma membrane was studied in UV-treated cells to investigate its involvement in apoptosis. It was studied in HL60 cells, in which DNA oligonucleosomic cleavage occurs, and in Molt-4 cells, which are characterised by a different fragmentation pattern. During the early stages of apoptosis, a membrane lipid rearrangement occurs, which involves phosphatidylserine translocation from the inner to the outer leaflet. This molecular alteration was investigated by annexin V-FITC binding, analysed by flow cytometry and confocal microscopy. It was correlated with transmission electron microscopy, subdiploid peak appearance and DNA fragmentation. Our data indicate that the plasma membrane represents an early apoptotic target, even if its alterations are not detectable by ultrastructural analysis, which indicates its good preservation until late apoptotic stages. In addition, the study of apoptotic cells with absent or inactivated endonuclease demonstrates the independence of this membrane mechanism from nuclear activity.

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Filippo Renò
    • 1
  • Sabrina Burattini
    • 1
  • Stefano Rossi
    • 1
  • Francesca Luchetti
    • 1
  • Marta Columbaro
    • 3
  • Spartaco Santi
    • 4
  • Stefano Papa
    • 1
  • E. Falcieri
    • 2
  1. 1.Centro di Citometria, Istituto di Scienze Morfologiche, Università di Urbino, Campus Scientifico ex-Sogesta, I-61029 Urbino, ItalyIT
  2. 2.Istituto di Anatomia e Fisiologia, Università di Urbino, I-61029 Urbino (PS), Italy, Tel.: +39-722-304267IT
  3. 3.Lab. Biologia Cellulare e Microscopia Elettronica, Istituto Ortopedici Rizzoli, Bologna, ItalyIT
  4. 4.Istituto di Citomorfologia Normale e Patologica, CNR, Bologna, ItalyIT