Histochemistry and Cell Biology

, Volume 107, Issue 3, pp 183–191

Immunolocalization of tissue non-specific alkaline phosphatase in mice

  • K. Hoshi
  • Norio Amizuka
  • Kimimitsu Oda
  • Yukio Ikehara
  • Hidehiro Ozawa
ORIGINAL PAPER

DOI: 10.1007/s004180050103

Cite this article as:
Hoshi, K., Amizuka, N., Oda, K. et al. Histochemistry (1997) 107: 183. doi:10.1007/s004180050103

Abstract

 Immunolocalization of tissue non-specific alkaline phosphatase (TNAP) was examined in murine tissues, employing a specific antiserum to TNAP on frozen sections, 50-μm tissue slices, and paraffin sections. TNAP was detected at high levels in hard tissues including bone, cartilage, and tooth. In bone tissue, the TNAP immunoreactivity was localized on the entire cell surface of preosteoblasts, as well as the basolateral cell membrane of osteoblasts. It was also localized on some resting chondrocytes and most of the proliferative and hypertrophic cells in cartilage. In the incisor, cells of the stratum intermedium, the subodontoblastic layer, the proximal portion of secretory ameloblasts, and the basolateral portion of odontoblasts showed particularly strong immunoreactivity. Immunoreactivity was observed in other soft tissues, such as the brush borders of proximal renal tubules in kidney, on cell membrane of the biliary canalicula in liver and in trophoblasts in the placenta. These immunolocalizations were quite similar to enzyme histochemical localizations. However, neither the submandibular gland nor the intestine, which both exhibited alkaline phosphatase activity by enzyme histochemistry, revealed immunoreactivity for TNAP. Therefore, immunocytohistochemical studies for TNAP enabled us to localize the TNAP isozyme, thus distinguishing it from other isozymes.

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • K. Hoshi
    • 1
  • Norio Amizuka
    • 1
  • Kimimitsu Oda
    • 3
  • Yukio Ikehara
    • 4
  • Hidehiro Ozawa
    • 1
  1. 1.1st Department of Oral Anatomy, Niigata University School of Dentistry, 5274, 2-Bancho, Gakkocho-dori, Niigata, 951 Japan Tel. +81-25-223-6161 ext. 4123; fax +81-25-228-8771 e-mail: pochi@dent.niigata-u.ac.jpJP
  2. 2.Department of Orthopaedic Surgery, Faculty of Medicine, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo, 113 JapanJP
  3. 3.Department of Biochemistry, Niigata University School of Dentistry, 5274, 2-Bancho, Gakkocho-dori, Niigata, 951 JapanJP
  4. 4.Department of Biochemistry, Fukuoka University School of Medicine, 7-45-1, Nanakuma, Jonan-ku, Fukuoka, 814-01 JapanJP