Histochemistry and Cell Biology

, Volume 139, Issue 4, pp 615–620

Transcription factor CREB is phosphorylated in human molar odontoblasts and cementoblasts in vivo

  • Franz-Josef Klinz
  • Yüksel Korkmaz
  • Britta Cho
  • Wolfgang H.-M. Raab
  • Klaus Addicks
Short communication

DOI: 10.1007/s00418-012-1047-8

Cite this article as:
Klinz, F., Korkmaz, Y., Cho, B. et al. Histochem Cell Biol (2013) 139: 615. doi:10.1007/s00418-012-1047-8

Abstract

A wide variety of stimuli can trigger activation of the transcription factor CREB (cAMP-responsive element binding protein), pointing toward a central role for CREB in the integration of various signaling inputs. No data are available on the expression and phosphorylation of CREB in mammalian teeth. Using immunohistochemical analysis of free-floating sections, we show here that CREB was strongly expressed and phosphorylated at Ser-133 within the nucleus of a subpopulation of adult human molar odontoblasts. Many dental pulp stromal cells and periodontal ligament fibroblasts expressed CREB and showed phosphorylation of CREB at Ser-133. In addition, cementoblasts displayed nuclear expression and phosphorylation of CREB at Ser-133. The epithelial rests of Malassez revealed strong nuclear expression of CREB, but phosphorylation at Ser-133 was variable. Our results provide the first evidence that the constitutively phosphorylated transcription factor CREB is involved in the biomineralization process of adult human molar odontoblasts and cementoblasts.

Keywords

BiomineralizationcAMP-responsive element binding proteinCementoblastEpithelial rests of MalassezOdontoblastPhospho-CREB Ser-133

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Franz-Josef Klinz
    • 1
  • Yüksel Korkmaz
    • 2
  • Britta Cho
    • 2
  • Wolfgang H.-M. Raab
    • 2
  • Klaus Addicks
    • 1
  1. 1.Department of Anatomy IUniversity of CologneCologneGermany
  2. 2.Department of Operative Dentistry, Periodontics and EndodonticsHeinrich-Heine-UniversityDüsseldorfGermany