Histochemistry and Cell Biology

, Volume 137, Issue 3, pp 319–327

Expression and distribution of symplekin regulates the assembly and function of the epithelial tight junction

Original Paper

DOI: 10.1007/s00418-011-0906-z

Cite this article as:
Chang, H., Zhang, C. & Cao, Y. Histochem Cell Biol (2012) 137: 319. doi:10.1007/s00418-011-0906-z

Abstract

Symplekin is multifunctional protein localized to both the tight junction and the nucleus with known roles in mRNA polyadenylation, proliferation, differentiation and tumorigenesis. Functions of symplekin at tight junctions have not been systematically investigated. In this study, increased expression of symplekin was observed during the formation of tight junctions in cultured HT-29 and HepG2 epithelial cells. Repression of symplekin by RNAi increased the permeability of epithelial monolayers, disrupted cellular polarity, and decreased the expression of the tight junction protein ZO-1. Moreover, symplekin was co-localized with ZO-1 at tight junctions and co-immunoprecipitated with ZO-1, indicating that ZO-1 and symplekin form complexes. In conclusion, symplekin expression regulates the assembly of tight junctions, thus helps to maintain the integrity of the epithelial monolayer and cellular polarity.

Keywords

SymplekinTight junctionPermeabilityPolarityZO-1

Supplementary material

418_2011_906_MOESM1_ESM.doc (32 kb)
Supplementary material 1 (DOC 32 kb)
418_2011_906_MOESM2_ESM.doc (28 kb)
Supplementary material 2 (DOC 28 kb)
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Supplementary material 3 (TIFF 570 kb)
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Supplementary material 4 (TIFF 997 kb)
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Supplementary material 5 (TIFF 1246 kb)

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  1. 1.Key Laboratory of Animal Models and Human Disease Mechanism of CAS and Yunnan Province, Kunming Institute of ZoologyChinese Academy of SciencesKunmingPeoples’ Republic of China
  2. 2.Graduate University of the Chinese Academy of SciencesBeijingChina