Histochemistry and Cell Biology

, Volume 133, Issue 4, pp 455–465

Differential expression of stem cell markers in human follicular bulge and interfollicular epidermal compartments

  • Shu Jiang
  • Longmei Zhao
  • Bhamini Purandare
  • Basil M. Hantash
Original Paper

DOI: 10.1007/s00418-010-0684-z

Cite this article as:
Jiang, S., Zhao, L., Purandare, B. et al. Histochem Cell Biol (2010) 133: 455. doi:10.1007/s00418-010-0684-z

Abstract

Although skin contains a number of stem cell repositories, their characterization has been hindered by a lack of specific markers and an unclear in vivo localization. In this study, we whole mounted single human scalp hair follicles and examined their profiles using in situ immunohistochemistry and multicolor immunofluorescence in search of markers to distinguish between stem cells residing in the interfollicular epidermis (IFE) and bulge. Our study revealed that expression of several biomarkers localized uniquely to the basal IFE (CD34 and CD117), bulge region (CD200), or both (CK15, CD49f, and CD29). In addition, we found that both basal IFE and bulge stem cells did not express CD71 or CD24 suggesting their potential utility as negative selection markers. Dermal papilla but not basal IFE or bulge stem cells expressed CD90, making it a potential positive selection marker for dermal hair follicle stem cells. The markers tested in this study may enable pursuit of cell sorting and purification strategies aimed at determining each stem cell population’s unique molecular signature.

Keywords

EpidermisHair follicleStem cellCell surface marker

Abbreviations

DP

Dermal papilla

HF

Hair follicle

IFE

Interfollicular epidermis

IRS

Inner root sheath

ORS

Outer root sheath

SG

Sebaceous glands

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Shu Jiang
    • 1
  • Longmei Zhao
    • 1
  • Bhamini Purandare
    • 2
  • Basil M. Hantash
    • 1
  1. 1.Division of Plastic Surgery, Department of SurgeryStanford University School of MedicineStanfordUSA
  2. 2.San Jose State UniversitySan JoseUSA