Histochemistry and Cell Biology

, Volume 133, Issue 4, pp 477–480

A pitfall in using BODIPY dyes to label lipid droplets for fluorescence microscopy

  • Yuki Ohsaki
  • Yuki Shinohara
  • Michitaka Suzuki
  • Toyoshi Fujimoto
Short communication

DOI: 10.1007/s00418-010-0678-x

Cite this article as:
Ohsaki, Y., Shinohara, Y., Suzuki, M. et al. Histochem Cell Biol (2010) 133: 477. doi:10.1007/s00418-010-0678-x

Abstract

The lipid droplet (LD) has become a focus of intense research. Fluorescence labeling is indispensable for the cell biological analysis of the LD, and a lipophilic fluorescence dye, BODIPY 493/503, which emits bright green fluorescence has been used extensively for LD labeling. The dye is convenient for double fluorescence labeling, but we noticed that it emits red fluorescence under certain conditions, which could lead to erroneous interpretations. We propose a protocol to preclude such a possibility.

Keywords

Lipid droplet BODIPY 

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Yuki Ohsaki
    • 1
  • Yuki Shinohara
    • 1
  • Michitaka Suzuki
    • 1
  • Toyoshi Fujimoto
    • 1
  1. 1.Department of Anatomy and Molecular Cell BiologyNagoya University Graduate School of MedicineNagoyaJapan