Original Paper

Histochemistry and Cell Biology

, 132:533

First online:

Characterization of mesenchymal stem cells from rat bone marrow: ultrastructural properties, differentiation potential and immunophenotypic markers

  • Erdal KaraozAffiliated withStem Cell and Gene Therapy Research and Application Center, Kocaeli University Email author 
  • , Ayça AksoyAffiliated withStem Cell and Gene Therapy Research and Application Center, Kocaeli University
  • , Selda AyhanAffiliated withStem Cell and Gene Therapy Research and Application Center, Kocaeli University
  • , Ayla Eker SarıboyacıAffiliated withStem Cell and Gene Therapy Research and Application Center, Kocaeli University
  • , Figen KaymazAffiliated withDepartment of Histology and Embryology, Faculty of Medicine, Hacettepe University
  • , Murat KasapAffiliated withStem Cell and Gene Therapy Research and Application Center, Kocaeli University

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Abstract

Bone marrow-derived mesenchymal stem cells (BM-MSCs) can differentiate into many lineages. Although the growing interest in BM-MSCs has led to a number of characterization studies, some important biochemical and immunohistochemical properties are still lacking. In this study, morphological and immunophenotypic properties of BM-MSCs were examined in detail. Differentiation potential and growth kinetics of adult rat BM-MSCs were also determined. Immunohistochemistry and RT-PCR results indicated that BM-MSCs expressed myogenic (desmin, myogenin, myosin IIa, and α-SMA), neurogenic (γ-enolase, MAP2a,b, c-fos, nestin, GFAP and beta III tubulin), and osteogenic (osteonectin, osteocalcin, osteopontin, Runx-2, BMP-2, BMP-4 and type I collagen) markers without stimulation towards differentiation. These expression patterns indicated why these cells can easily differentiate into multiple lineages both in vitro and in vivo. Ultrastructural characteristics of rBM-MSCs showed more developed and metabolically active cells.

Keywords

Rat Bone marrow Stem cell Differentiation Proliferation Ultrastructure Immunophenotype